Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   COB47_RS08480 Genome accession   NC_014392
Coordinates   1823990..1825045 (-) Length   351 a.a.
NCBI ID   WP_013290967.1    Uniprot ID   -
Organism   Caldicellulosiruptor obsidiansis OB47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1818990..1830045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COB47_RS08445 (COB47_1683) - 1819540..1820112 (-) 573 WP_013290960.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  COB47_RS08450 (COB47_1684) - 1820109..1820663 (-) 555 WP_013290961.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  COB47_RS08455 (COB47_1685) - 1820653..1820943 (-) 291 WP_013290962.1 late competence development ComFB family protein -
  COB47_RS08460 (COB47_1686) - 1820951..1821727 (-) 777 WP_013290963.1 A24 family peptidase -
  COB47_RS08465 (COB47_1687) - 1821780..1822217 (-) 438 WP_013290964.1 type II secretion system protein -
  COB47_RS08470 (COB47_1688) - 1822253..1822669 (-) 417 WP_013290965.1 type II secretion system protein -
  COB47_RS08475 (COB47_1689) - 1822708..1823919 (-) 1212 WP_013290966.1 type II secretion system F family protein -
  COB47_RS08480 (COB47_1690) pilT 1823990..1825045 (-) 1056 WP_013290967.1 type IV pilus twitching motility protein PilT Machinery gene
  COB47_RS08485 (COB47_1691) - 1825099..1826811 (-) 1713 WP_013290968.1 GspE/PulE family protein -
  COB47_RS08490 (COB47_1692) - 1826839..1830036 (-) 3198 WP_013290969.1 response regulator -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39679.76 Da        Isoelectric Point: 7.2523

>NTDB_id=38067 COB47_RS08480 WP_013290967.1 1823990..1825045(-) (pilT) [Caldicellulosiruptor obsidiansis OB47]
MDINSILKEAFLKEASDIHITPGVPPIYRIHGRLVRTDDSILTPEMVEEFVRQITNENQFKILEQKGEIDFSYSIKGVSR
FRVNVYRQRGSYSIAFRIIPVNIPPFETLGLPPVLKEFTKLNKGLVLVTGPTGSGKSTTLASLIDIINKERDVHIITLED
PIEYLHRHNKSIINQREIGSDTLSFADALRAALREDPDVILVGEMRDLETIAIALTAAETGHLVFSTLHTIGAAKTIDRI
IDVFPPHQQQQIRIQLSTVLQGVVSQQLLTRRDGKGRVVATEVMIVNPAIRNLIREAKTYQIQSIIQTHQRQGMITMEQS
LIDLYKRGLISREDAFNYATDFDFMQRLLSA

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=38067 COB47_RS08480 WP_013290967.1 1823990..1825045(-) (pilT) [Caldicellulosiruptor obsidiansis OB47]
ATGGATATTAATTCAATTCTCAAAGAAGCATTTCTCAAGGAGGCTTCAGATATACATATTACGCCAGGTGTTCCTCCAAT
TTATCGAATACACGGCAGATTAGTACGGACAGATGATTCAATTCTGACACCTGAGATGGTGGAGGAGTTTGTGCGACAGA
TCACAAACGAAAACCAGTTTAAAATTCTTGAGCAGAAAGGAGAGATAGACTTTTCGTACAGTATAAAGGGAGTAAGTAGA
TTCAGGGTAAATGTTTACAGACAAAGAGGTTCGTATTCTATAGCTTTTAGAATAATTCCTGTAAATATACCACCGTTTGA
AACACTTGGCCTTCCACCAGTTTTGAAAGAATTTACAAAATTGAATAAAGGGCTTGTTTTAGTTACAGGTCCAACTGGTT
CGGGTAAGTCAACAACACTGGCATCGCTGATTGACATAATCAACAAAGAAAGAGATGTACATATAATCACATTAGAGGAC
CCCATAGAGTATTTGCACAGGCACAACAAGAGTATTATCAATCAAAGAGAGATAGGTAGTGACACACTCAGCTTTGCAGA
CGCGCTGAGGGCGGCTTTGAGAGAAGATCCTGATGTAATCCTGGTTGGTGAGATGAGGGATTTGGAGACAATTGCGATAG
CTTTAACGGCTGCTGAAACAGGACACCTGGTGTTTTCCACACTTCACACAATTGGAGCGGCAAAGACAATAGACCGTATC
ATTGACGTTTTTCCACCGCATCAACAGCAGCAGATAAGAATTCAGCTATCAACAGTTTTGCAGGGAGTTGTGTCTCAGCA
GCTTTTGACCCGTCGTGATGGTAAAGGCAGGGTTGTTGCAACAGAGGTAATGATAGTAAATCCGGCCATAAGAAACCTCA
TTAGGGAGGCTAAAACGTATCAGATTCAATCAATTATTCAGACGCATCAGCGACAGGGCATGATAACAATGGAACAGTCA
CTCATAGACTTGTACAAAAGAGGACTTATCAGTCGTGAAGATGCGTTCAACTATGCTACTGACTTTGATTTTATGCAAAG
ACTGCTCAGTGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.724

99.145

0.513
  pilT Neisseria meningitidis 8013

51.445

98.575

0.507
  pilT Acinetobacter nosocomialis M2

52.537

95.442

0.501
  pilT Acinetobacter baumannii D1279779

52.537

95.442

0.501
  pilT Acinetobacter baumannii strain A118

52.537

95.442

0.501
  pilT Legionella pneumophila strain Lp02

51.917

96.581

0.501
  pilT Legionella pneumophila strain ERS1305867

51.917

96.581

0.501
  pilT Neisseria gonorrhoeae MS11

50.725

98.291

0.499
  pilT Pseudomonas aeruginosa PAK

51.642

95.442

0.493
  pilT Acinetobacter baylyi ADP1

52.266

94.302

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.796

95.157

0.493
  pilT Vibrio cholerae strain A1552

51.796

95.157

0.493
  pilT Pseudomonas stutzeri DSM 10701

51.343

95.442

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.36

100

0.416
  pilU Acinetobacter baylyi ADP1

41.176

96.866

0.399
  pilU Vibrio cholerae strain A1552

38.682

99.43

0.385

Multiple sequence alignment