Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   acear_RS09115 Genome accession   NC_014378
Coordinates   1859903..1860949 (-) Length   348 a.a.
NCBI ID   WP_013278723.1    Uniprot ID   D9QRY7
Organism   Acetohalobium arabaticum DSM 5501     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1854903..1865949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  acear_RS09095 (Acear_1769) - 1855327..1857108 (-) 1782 WP_013278719.1 O-antigen ligase family protein -
  acear_RS12955 (Acear_1770) - 1857815..1858198 (-) 384 WP_013278720.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  acear_RS12960 (Acear_1771) - 1858243..1858638 (-) 396 WP_013278721.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  acear_RS09110 (Acear_1772) - 1858661..1859788 (-) 1128 WP_245526681.1 type II secretion system F family protein -
  acear_RS09115 (Acear_1773) pilT 1859903..1860949 (-) 1047 WP_013278723.1 type IV pilus twitching motility protein PilT Machinery gene
  acear_RS09120 (Acear_1774) gspE 1860964..1862661 (-) 1698 WP_013278724.1 type II secretion system ATPase GspE -
  acear_RS09125 (Acear_1775) aroE 1862766..1863644 (-) 879 WP_013278725.1 shikimate dehydrogenase -
  acear_RS09130 (Acear_1776) - 1864617..1865513 (-) 897 WP_013278726.1 DMT family transporter -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38746.35 Da        Isoelectric Point: 6.0125

>NTDB_id=38029 acear_RS09115 WP_013278723.1 1859903..1860949(-) (pilT) [Acetohalobium arabaticum DSM 5501]
MELDDLLREGIRVGASDIHLTVGIEPTARINGNLRKLCDSKINSDKINGLVAGMLGSNEEEIYKETGEVDFAYHLDNYRF
RVNVFKQRGNPAAVLRIIPNEILSLEELGLPEKLKELALKPRGLFLVTGPTGSGKSTTLASMIDLVNSKMDKHIITLEDP
IEYVHQHKRSIINQRAVGKDTDNFANGLRSALRQDPDIILVGEMRDLETISTAITAAETGHLVLATLHTNNASETIERII
NVFPAHQQTQVRTQLALTLEGVLSQQLLPTIDNLDRVVATELMIANSAVRNLIREDKTHQLESVMQTGSDEGMHTMDYSL
RDLYLNGKISRKMALERAVNVETLKKQI

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=38029 acear_RS09115 WP_013278723.1 1859903..1860949(-) (pilT) [Acetohalobium arabaticum DSM 5501]
ATGGAATTAGATGATCTTCTGCGGGAAGGGATTAGAGTAGGAGCTTCTGATATTCATCTGACTGTAGGAATTGAACCGAC
GGCGCGAATTAATGGGAATTTACGGAAGTTATGTGATAGCAAGATAAATTCTGACAAAATTAATGGTTTAGTGGCAGGAA
TGCTGGGGTCTAATGAAGAAGAAATATATAAGGAAACAGGTGAAGTAGATTTTGCTTATCATCTGGATAACTATCGCTTT
CGTGTTAATGTTTTTAAACAACGGGGGAATCCGGCGGCAGTTCTGCGGATTATTCCTAATGAGATTCTGAGTCTAGAGGA
GTTAGGACTGCCGGAGAAATTAAAAGAACTGGCTTTAAAGCCGCGTGGTCTCTTTTTAGTAACGGGACCTACTGGCAGCG
GGAAATCGACCACTTTAGCTTCAATGATTGATCTGGTTAACAGTAAGATGGACAAGCATATCATTACTTTAGAAGATCCA
ATTGAATATGTTCATCAGCATAAACGGAGTATTATCAATCAGCGGGCTGTAGGAAAGGATACTGATAATTTTGCTAATGG
TTTGAGATCGGCTCTAAGACAGGATCCCGATATAATTTTAGTCGGTGAGATGAGAGATTTAGAAACTATCTCGACGGCAA
TTACTGCTGCTGAAACTGGCCATCTGGTGTTGGCTACTCTACATACTAATAATGCTTCGGAAACAATTGAGCGAATCATT
AATGTCTTTCCGGCCCATCAACAGACTCAGGTTAGAACTCAGCTGGCATTAACACTGGAAGGGGTCTTATCCCAGCAGCT
GCTGCCGACAATTGATAATCTGGATAGAGTAGTGGCTACGGAGCTCATGATTGCTAATTCAGCAGTCAGAAACTTAATTA
GAGAAGATAAGACTCATCAGTTGGAATCAGTAATGCAGACTGGAAGTGATGAAGGAATGCATACGATGGATTATTCGCTG
CGTGATCTATACTTAAACGGCAAGATAAGTAGAAAAATGGCTCTAGAACGGGCGGTTAATGTTGAGACCCTTAAGAAGCA
GATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D9QRY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.009

99.713

0.509
  pilT Neisseria meningitidis 8013

51.304

99.138

0.509
  pilT Neisseria gonorrhoeae MS11

51.014

99.138

0.506
  pilT Legionella pneumophila strain Lp02

50.147

97.989

0.491
  pilT Legionella pneumophila strain ERS1305867

50.147

97.989

0.491
  pilT Acinetobacter baylyi ADP1

48.986

99.138

0.486
  pilT Acinetobacter baumannii strain A118

48.406

99.138

0.48
  pilT Acinetobacter baumannii D1279779

48.406

99.138

0.48
  pilT Acinetobacter nosocomialis M2

48.406

99.138

0.48
  pilT Pseudomonas aeruginosa PAK

48.378

97.414

0.471
  pilT Pseudomonas stutzeri DSM 10701

47.246

99.138

0.468
  pilT Vibrio cholerae strain A1552

48.485

94.828

0.46
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.485

94.828

0.46
  pilU Acinetobacter baylyi ADP1

42.857

100

0.431
  pilU Vibrio cholerae strain A1552

42.319

99.138

0.42
  pilU Pseudomonas stutzeri DSM 10701

42.773

97.414

0.417

Multiple sequence alignment