Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TOCE_RS09705 Genome accession   NC_014377
Coordinates   1935950..1936978 (-) Length   342 a.a.
NCBI ID   WP_013276678.1    Uniprot ID   -
Organism   Thermosediminibacter oceani DSM 16646     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1930950..1941978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOCE_RS09680 (Toce_1924) - 1931939..1932481 (-) 543 WP_013276673.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TOCE_RS09685 (Toce_1925) - 1932478..1932906 (-) 429 WP_013276674.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TOCE_RS09690 (Toce_1926) - 1933003..1933758 (-) 756 WP_013276675.1 A24 family peptidase -
  TOCE_RS09695 (Toce_1927) - 1933945..1934460 (-) 516 WP_013276676.1 type II secretion system protein GspG -
  TOCE_RS09700 (Toce_1928) - 1934556..1935770 (-) 1215 WP_013276677.1 type II secretion system F family protein -
  TOCE_RS09705 (Toce_1929) pilT 1935950..1936978 (-) 1029 WP_013276678.1 type IV pilus twitching motility protein PilT Machinery gene
  TOCE_RS09710 (Toce_1930) - 1937003..1938700 (-) 1698 WP_013276679.1 GspE/PulE family protein -
  TOCE_RS09715 (Toce_1931) - 1938820..1939284 (-) 465 WP_013276680.1 GNAT family N-acetyltransferase -
  TOCE_RS09720 (Toce_1932) - 1939435..1939980 (+) 546 WP_013276681.1 XRE family transcriptional regulator -
  TOCE_RS09725 (Toce_1933) - 1940035..1941111 (-) 1077 WP_013276682.1 Xaa-Pro peptidase family protein -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 37585.19 Da        Isoelectric Point: 6.9128

>NTDB_id=38008 TOCE_RS09705 WP_013276678.1 1935950..1936978(-) (pilT) [Thermosediminibacter oceani DSM 16646]
MHLREILGEAIKKGASDIHLTVGLPPVFRHHGRLEPQGQWPPLTASDTEALAREILGERWEEFIERKELDLAYSAAGLGR
FRVNIFHQRGSIGAALRLVGDVIHSLDELGLPPVVGELADQPHGLVLVTGPTGSGKTTTLAAMIDRINERRSCHIITLED
PIEYLHTHKRSIVNQREIGSDSPSFASALRAALRQDPDVILVGEMRDLETVATAITAAETGHLVLATLHTGSAVQSIDRI
IDVFPPHQQNQIRIQLSDTLAGVITQQLLPRADGKGRVVAVEVLIATPAVRNLIREGKTHQIVSLMQTGGRFGMQTMEMS
LRQLREKGLIHPDLYQLKKDFG

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=38008 TOCE_RS09705 WP_013276678.1 1935950..1936978(-) (pilT) [Thermosediminibacter oceani DSM 16646]
ATGCATTTGCGGGAAATACTGGGAGAAGCCATAAAAAAAGGGGCATCGGATATACATCTGACGGTGGGTTTACCGCCGGT
CTTCCGGCATCACGGACGGCTCGAACCTCAGGGGCAGTGGCCTCCTTTGACTGCTTCTGATACTGAAGCACTGGCAAGGG
AGATTTTGGGTGAACGGTGGGAAGAATTTATTGAGCGAAAGGAACTGGACCTGGCCTACTCGGCAGCGGGATTGGGGCGT
TTTCGGGTAAATATTTTTCACCAGCGCGGAAGTATAGGGGCTGCCTTACGCCTGGTTGGCGATGTTATTCACAGCCTTGA
CGAGTTGGGGCTACCCCCGGTGGTAGGCGAGCTGGCCGATCAGCCCCACGGCCTGGTCCTGGTTACCGGGCCTACCGGCA
GCGGTAAGACCACGACTCTGGCGGCCATGATCGACCGGATTAACGAGCGGCGCAGCTGTCACATAATTACGCTGGAGGAC
CCCATAGAGTACCTGCATACGCATAAAAGGAGTATCGTGAACCAGCGGGAAATCGGAAGTGACTCTCCTTCCTTTGCGAG
CGCTTTAAGGGCTGCCCTGCGCCAGGACCCCGACGTTATTCTGGTGGGCGAGATGCGGGACCTGGAGACGGTAGCCACGG
CAATTACTGCGGCGGAAACCGGGCACCTGGTGCTGGCTACCCTTCATACCGGCAGTGCTGTCCAGAGCATTGACCGGATT
ATAGACGTTTTTCCTCCGCACCAGCAGAACCAGATCAGAATCCAGCTTTCGGATACTCTGGCCGGAGTCATTACCCAGCA
ACTGCTGCCCCGGGCTGACGGCAAAGGACGGGTGGTGGCTGTGGAAGTTTTGATTGCTACCCCTGCTGTGCGCAATCTTA
TAAGGGAAGGGAAAACCCACCAGATCGTTTCTTTGATGCAGACGGGCGGCCGATTCGGAATGCAGACCATGGAAATGTCG
CTCAGGCAATTGCGTGAAAAGGGATTAATTCACCCGGATTTATATCAATTGAAGAAGGATTTTGGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

58.084

97.661

0.567
  pilT Pseudomonas aeruginosa PAK

50

97.661

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.673

99.123

0.482
  pilT Vibrio cholerae strain A1552

48.673

99.123

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.961

98.538

0.482
  pilT Legionella pneumophila strain Lp02

49.102

97.661

0.48
  pilT Legionella pneumophila strain ERS1305867

49.102

97.661

0.48
  pilT Acinetobacter baylyi ADP1

49.096

97.076

0.477
  pilT Acinetobacter baumannii D1279779

48.048

97.368

0.468
  pilT Acinetobacter baumannii strain A118

48.048

97.368

0.468
  pilT Acinetobacter nosocomialis M2

48.048

97.368

0.468
  pilT Neisseria meningitidis 8013

47.761

97.953

0.468
  pilT Neisseria gonorrhoeae MS11

47.605

97.661

0.465
  pilU Pseudomonas stutzeri DSM 10701

43.988

99.708

0.439
  pilU Vibrio cholerae strain A1552

44.615

95.029

0.424
  pilU Acinetobacter baylyi ADP1

41.692

96.784

0.404

Multiple sequence alignment