Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   UC8_RS12515 Genome accession   NZ_CP042914
Coordinates   3493640..3494746 (-) Length   368 a.a.
NCBI ID   WP_068130521.1    Uniprot ID   -
Organism   Roseimaritima ulvae strain UC8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3488640..3499746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UC8_RS12500 (UC8_25380) - 3488913..3490079 (-) 1167 WP_068130513.1 type II secretion system protein -
  UC8_RS12505 (UC8_25390) - 3490306..3491745 (-) 1440 WP_068130515.1 type II secretion system F family protein -
  UC8_RS12510 (UC8_25400) - 3491923..3493638 (-) 1716 WP_068130518.1 GspE/PulE family protein -
  UC8_RS12515 (UC8_25410) pilT 3493640..3494746 (-) 1107 WP_068130521.1 type IV pilus twitching motility protein PilT Machinery gene
  UC8_RS12520 (UC8_25420) - 3495151..3496908 (-) 1758 WP_068130527.1 GspE/PulE family protein -
  UC8_RS12525 (UC8_25440) - 3497575..3498951 (+) 1377 WP_068130530.1 DUF1501 domain-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40467.50 Da        Isoelectric Point: 6.4224

>NTDB_id=379395 UC8_RS12515 WP_068130521.1 3493640..3494746(-) (pilT) [Roseimaritima ulvae strain UC8]
MATVLIDKLLQAAVKQGVSDIHIVVGQPPVFRLHGRMRKLETKVLENEDTVGLMKSITPERCQRELQETGSADFGFAFGE
MARFRVSVFKQRGSISMVLRQIPNDKLTPEQLGLPDSVVKLVMRPRGLFLVTGPTGSGKSTTLASLVNLINETHDHHIIT
IEDPIEFYHEHIRSTVNQREVGVDVPSFAEAIRRALRQDPDVILVGELRDLETIEAAISAAETGHIVFGTLHTNSAQGTV
NRVIDAFPGNLQDQIRTQLASSLIGVVAQTLLPRIGGGRVAAYEVLVVTPGIGNLIRENKTFRINSAIQTGAKLGMQLMD
DALFKLWSEEKVTVEDVLSKAQRPDDLAKRIVQARRGVIDEPEVLGDD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=379395 UC8_RS12515 WP_068130521.1 3493640..3494746(-) (pilT) [Roseimaritima ulvae strain UC8]
ATGGCTACCGTGCTGATTGACAAACTGTTGCAAGCTGCGGTCAAGCAGGGTGTGAGCGATATCCATATTGTGGTCGGCCA
GCCGCCGGTGTTCCGCTTGCACGGCCGGATGCGGAAGCTGGAAACCAAGGTGTTGGAAAACGAAGACACCGTGGGGCTGA
TGAAAAGCATCACGCCGGAGCGTTGCCAACGTGAACTCCAAGAGACCGGCAGCGCCGACTTCGGGTTCGCTTTCGGCGAG
ATGGCTCGCTTCCGCGTGTCGGTGTTCAAGCAGCGCGGCTCGATCTCGATGGTGCTGCGGCAGATCCCCAACGACAAACT
GACCCCCGAGCAGCTGGGGTTGCCCGATTCTGTCGTCAAATTGGTGATGCGGCCACGTGGACTTTTCCTGGTCACCGGCC
CCACTGGTAGCGGCAAGAGCACGACGCTGGCCAGCCTGGTGAACCTGATCAACGAGACGCACGATCACCACATCATCACG
ATCGAGGACCCGATCGAGTTTTATCACGAACACATTCGCAGCACGGTCAACCAACGCGAGGTGGGCGTCGACGTGCCCAG
CTTTGCCGAAGCGATTCGGCGAGCTTTGCGGCAGGACCCGGACGTGATCCTGGTCGGCGAGCTGCGGGATTTGGAAACGA
TTGAAGCGGCCATCAGCGCGGCCGAAACCGGTCACATTGTGTTTGGCACCCTGCACACCAACAGTGCCCAGGGCACGGTC
AACCGCGTGATCGATGCTTTCCCAGGCAACCTGCAGGACCAGATCCGCACCCAGTTGGCCAGTTCGCTGATCGGCGTCGT
GGCCCAGACCCTGCTGCCTCGCATCGGCGGCGGCCGCGTGGCGGCTTACGAGGTGTTGGTGGTCACGCCGGGCATCGGCA
ACCTGATTCGCGAAAACAAAACCTTCCGCATCAATAGTGCGATCCAAACCGGTGCCAAGCTGGGCATGCAGCTGATGGAC
GACGCCCTATTCAAGTTGTGGTCGGAAGAAAAAGTCACCGTCGAAGACGTGTTGAGCAAAGCTCAGCGGCCGGACGACCT
GGCCAAGCGGATCGTGCAAGCGCGACGCGGGGTCATCGACGAACCCGAAGTACTTGGAGACGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.847

96.196

0.489
  pilT Pseudomonas aeruginosa PAK

48.538

92.935

0.451
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.552

91.033

0.451
  pilT Vibrio cholerae strain A1552

49.552

91.033

0.451
  pilT Acinetobacter nosocomialis M2

48.246

92.935

0.448
  pilT Acinetobacter baumannii strain A118

47.953

92.935

0.446
  pilT Acinetobacter baumannii D1279779

47.953

92.935

0.446
  pilT Pseudomonas stutzeri DSM 10701

47.661

92.935

0.443
  pilT Legionella pneumophila strain ERS1305867

49.39

89.13

0.44
  pilT Legionella pneumophila strain Lp02

49.39

89.13

0.44
  pilT Acinetobacter baylyi ADP1

46.491

92.935

0.432
  pilT Neisseria meningitidis 8013

45.349

93.478

0.424
  pilT Neisseria gonorrhoeae MS11

45.058

93.478

0.421
  pilU Pseudomonas stutzeri DSM 10701

40.816

93.207

0.38
  pilU Acinetobacter baylyi ADP1

39.368

94.565

0.372
  pilU Vibrio cholerae strain A1552

37.96

95.924

0.364

Multiple sequence alignment