Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HSERO_RS19000 Genome accession   NC_014323
Coordinates   4356910..4357965 (+) Length   351 a.a.
NCBI ID   WP_013235744.1    Uniprot ID   D8IRT9
Organism   Herbaspirillum seropedicae SmR1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4353665..4368814 4356910..4357965 within 0


Gene organization within MGE regions


Location: 4353665..4368814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSERO_RS18975 (Hsero_3799) - 4353665..4353955 (-) 291 WP_013235739.1 hypothetical protein -
  HSERO_RS18980 (Hsero_3800) - 4354239..4354466 (-) 228 WP_013235740.1 hypothetical protein -
  HSERO_RS18985 (Hsero_3801) - 4354502..4355266 (-) 765 WP_013235741.1 peptidoglycan DD-metalloendopeptidase family protein -
  HSERO_RS18990 (Hsero_3802) proC 4355266..4356078 (-) 813 WP_013235742.1 pyrroline-5-carboxylate reductase -
  HSERO_RS18995 (Hsero_3803) - 4356126..4356827 (-) 702 WP_013235743.1 YggS family pyridoxal phosphate-dependent enzyme -
  HSERO_RS19000 (Hsero_3804) pilT 4356910..4357965 (+) 1056 WP_013235744.1 type IV pilus twitching motility protein PilT Machinery gene
  HSERO_RS19005 (Hsero_3805) - 4358014..4358499 (+) 486 WP_013235745.1 glutathione peroxidase -
  HSERO_RS19010 (Hsero_3806) ilvC 4358635..4359651 (-) 1017 WP_013235746.1 ketol-acid reductoisomerase -
  HSERO_RS19015 (Hsero_3807) - 4359701..4360858 (-) 1158 WP_041311191.1 sigma-54-dependent Fis family transcriptional regulator -
  HSERO_RS19020 (Hsero_3808) - 4361049..4361951 (+) 903 WP_013235748.1 LysR family transcriptional regulator -
  HSERO_RS19025 (Hsero_3809) - 4361973..4363082 (-) 1110 WP_013235749.1 DUF6708 domain-containing protein -
  HSERO_RS19030 - 4363075..4364154 (-) 1080 WP_041311192.1 DUF6708 domain-containing protein -
  HSERO_RS19035 (Hsero_3811) - 4364147..4365235 (-) 1089 WP_013235751.1 DUF6708 domain-containing protein -
  HSERO_RS19040 (Hsero_3812) - 4365228..4366310 (-) 1083 WP_013235752.1 DUF6708 domain-containing protein -
  HSERO_RS19045 (Hsero_3813) - 4366313..4368814 (-) 2502 WP_013235753.1 T6SS effector BTH_I2691 family protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 37364.33 Da        Isoelectric Point: 8.8235

>NTDB_id=37821 HSERO_RS19000 WP_013235744.1 4356910..4357965(+) (pilT) [Herbaspirillum seropedicae SmR1]
MDIAVLLAFAVRNQASDLHLCAGMPPLLRVHGALRRVNLQAPTGPQLQQWLQALMPAALHPRYAQGRECDFAIELAALGR
FRVHAFLRQGGPAVAIRHLGREPPTLAQLGAPPLCAELAERAGGLVLVTGPTGAGKSSTLAAMIRHLNETRALHILTIED
PIEFIHTPQRALISQRELGTHTADLPAALRAALRADPDVLLVGELRDADTIRLALTAAETGHLVLGTLHAASAARTVERM
LDVFPAGEKEPARAMLAESLQGVIAQTLLPTADGRARVAAHELLVATPAVRNLIREGRSAQLLSLMQAGAAQGMQTLEAA
LQRLHQQGRISAQTLRQHLGQEPQAGPGMAV

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=37821 HSERO_RS19000 WP_013235744.1 4356910..4357965(+) (pilT) [Herbaspirillum seropedicae SmR1]
ATGGACATTGCCGTGCTACTGGCATTTGCCGTCAGAAATCAGGCCTCCGACCTGCATCTATGCGCCGGCATGCCGCCCCT
GCTGCGGGTCCACGGGGCGCTGCGCCGGGTCAACCTGCAAGCCCCCACCGGCCCCCAGCTGCAACAATGGCTGCAGGCGC
TCATGCCTGCGGCCCTGCATCCGCGCTACGCCCAGGGGCGTGAATGCGACTTCGCCATCGAGCTGGCCGCGCTGGGGCGC
TTTCGCGTCCATGCCTTCCTGCGCCAGGGCGGACCTGCCGTCGCCATCCGCCACCTGGGCCGCGAACCGCCGACCCTGGC
GCAACTGGGCGCACCGCCCCTCTGCGCCGAGCTGGCCGAGCGTGCAGGCGGGCTGGTGCTGGTGACGGGCCCGACCGGCG
CGGGCAAGAGCAGCACGCTGGCGGCCATGATCCGCCACCTCAATGAAACCCGCGCCCTGCATATCCTGACCATCGAAGAC
CCGATCGAGTTCATCCACACGCCGCAACGCGCCCTGATCAGCCAGCGCGAACTGGGCACCCACACGGCGGATCTGCCCGC
CGCGCTGCGGGCCGCCTTGCGCGCCGATCCCGACGTATTGCTGGTGGGCGAACTGCGCGACGCCGACACCATCCGCTTAG
CGCTCACCGCCGCCGAAACCGGCCATCTGGTGCTGGGAACCCTGCATGCCGCCTCGGCGGCGCGCACCGTGGAGCGCATG
CTCGACGTCTTCCCGGCGGGCGAGAAGGAACCAGCGCGGGCCATGCTGGCCGAGTCGCTGCAAGGAGTGATTGCACAAAC
CCTGCTGCCCACCGCCGATGGCCGCGCCCGGGTGGCGGCGCACGAACTGCTGGTGGCCACGCCGGCCGTGCGCAATCTCA
TCCGCGAAGGTCGCAGCGCGCAATTGCTGTCGCTGATGCAGGCCGGCGCGGCGCAAGGCATGCAGACGCTGGAAGCCGCC
CTGCAGCGCCTGCATCAGCAAGGCCGTATCAGCGCGCAGACACTGCGCCAGCACCTGGGGCAGGAGCCTCAAGCAGGCCC
TGGCATGGCGGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D8IRT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

54.006

96.011

0.519
  pilT Neisseria gonorrhoeae MS11

53.709

96.011

0.516
  pilT Acinetobacter baumannii strain A118

52.083

95.726

0.499
  pilT Acinetobacter nosocomialis M2

52.083

95.726

0.499
  pilT Acinetobacter baumannii D1279779

52.083

95.726

0.499
  pilT Acinetobacter baylyi ADP1

51.786

95.726

0.496
  pilT Legionella pneumophila strain Lp02

52.096

95.157

0.496
  pilT Legionella pneumophila strain ERS1305867

52.096

95.157

0.496
  pilT Vibrio cholerae strain A1552

51.327

96.581

0.496
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.327

96.581

0.496
  pilT Pseudomonas stutzeri DSM 10701

49.853

97.151

0.484
  pilT Pseudomonas aeruginosa PAK

50.148

96.011

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.177

93.162

0.43
  pilU Vibrio cholerae strain A1552

39.94

94.872

0.379
  pilU Pseudomonas stutzeri DSM 10701

39.222

95.157

0.373
  pilU Acinetobacter baylyi ADP1

38.855

94.587

0.368

Multiple sequence alignment