Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FR888_RS22500 Genome accession   NZ_CP042512
Coordinates   4616588..4617610 (-) Length   340 a.a.
NCBI ID   WP_055316112.1    Uniprot ID   A0AAU8Y949
Organism   Serratia marcescens strain E28     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4611588..4622610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR888_RS22470 (FR888_22465) - 4612389..4612901 (+) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -
  FR888_RS22475 (FR888_22470) endA 4613003..4613698 (+) 696 WP_047729855.1 deoxyribonuclease I -
  FR888_RS22480 (FR888_22475) rsmE 4613768..4614499 (+) 732 WP_033635938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FR888_RS22485 (FR888_22480) gshB 4614510..4615460 (+) 951 WP_047729854.1 glutathione synthase -
  FR888_RS22490 (FR888_22485) - 4615606..4616169 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  FR888_RS22495 (FR888_22490) ruvX 4616169..4616591 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  FR888_RS22500 (FR888_22495) pilT 4616588..4617610 (-) 1023 WP_055316112.1 type IV pilus twitching motility protein PilT Machinery gene
  FR888_RS22505 (FR888_22500) - 4617631..4618338 (+) 708 WP_048234668.1 YggS family pyridoxal phosphate-dependent enzyme -
  FR888_RS22510 (FR888_22505) proC 4618358..4619179 (+) 822 WP_048234669.1 pyrroline-5-carboxylate reductase -
  FR888_RS22515 (FR888_22510) - 4619211..4619765 (+) 555 WP_047729850.1 YggT family protein -
  FR888_RS22520 (FR888_22515) yggU 4619762..4620052 (+) 291 WP_047729849.1 DUF167 family protein YggU -
  FR888_RS22525 (FR888_22520) - 4620070..4620663 (+) 594 WP_038879287.1 XTP/dITP diphosphatase -
  FR888_RS22530 (FR888_22525) hemW 4620656..4621798 (+) 1143 WP_060435163.1 radical SAM family heme chaperone HemW -
  FR888_RS28915 (FR888_22530) - 4621836..4621919 (-) 84 Protein_4402 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36367.65 Da        Isoelectric Point: 7.0670

>NTDB_id=378205 FR888_RS22500 WP_055316112.1 4616588..4617610(-) (pilT) [Serratia marcescens strain E28]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAASTLTAQGVQALCDGLLSAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDTALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=378205 FR888_RS22500 WP_055316112.1 4616588..4617610(-) (pilT) [Serratia marcescens strain E28]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGTCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAGTGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGCCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTTCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGTAGCCGACGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGTCACAGCTTCGA
TACGGCGTTGCGCGCTGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTTGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACTGGCCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGATGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.939

97.059

0.524
  pilT Vibrio cholerae strain A1552

53.939

97.059

0.524
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

49.552

98.529

0.488
  pilT Neisseria gonorrhoeae MS11

49.547

97.353

0.482
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Legionella pneumophila strain ERS1305867

48.64

97.353

0.474
  pilT Legionella pneumophila strain Lp02

48.64

97.353

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365

Multiple sequence alignment