Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FRD01_RS21315 Genome accession   NZ_CP042467
Coordinates   5192248..5193357 (+) Length   369 a.a.
NCBI ID   WP_146962961.1    Uniprot ID   A0A5B8XXL5
Organism   Microvenator marinus strain V1718     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 5190475..5207144 5192248..5193357 within 0


Gene organization within MGE regions


Location: 5190475..5207144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRD01_RS21310 (FRD01_21310) pilB 5190475..5192181 (+) 1707 WP_146962960.1 type IV-A pilus assembly ATPase PilB -
  FRD01_RS21315 (FRD01_21315) pilT 5192248..5193357 (+) 1110 WP_146962961.1 type IV pilus twitching motility protein PilT Machinery gene
  FRD01_RS21320 (FRD01_21320) - 5193376..5194593 (+) 1218 WP_146962962.1 type II secretion system F family protein -
  FRD01_RS21325 (FRD01_21325) - 5194590..5196191 (+) 1602 WP_146962963.1 ATP-binding protein -
  FRD01_RS21330 (FRD01_21330) - 5196188..5197600 (+) 1413 WP_249755806.1 sigma-54 dependent transcriptional regulator -
  FRD01_RS21335 (FRD01_21335) - 5197671..5198144 (+) 474 WP_146962964.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FRD01_RS24760 (FRD01_21340) - 5198293..5198952 (+) 660 WP_283808671.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FRD01_RS21345 (FRD01_21345) - 5199178..5199816 (+) 639 WP_146962965.1 hypothetical protein -
  FRD01_RS21350 (FRD01_21350) - 5199816..5200739 (+) 924 WP_146962966.1 ABC transporter ATP-binding protein -
  FRD01_RS21355 (FRD01_21355) - 5200736..5201539 (+) 804 WP_146962967.1 ABC transporter permease subunit -
  FRD01_RS21360 (FRD01_21360) xerD 5201605..5202501 (-) 897 WP_249755807.1 site-specific tyrosine recombinase XerD -
  FRD01_RS21365 (FRD01_21365) - 5202584..5204734 (+) 2151 WP_249755808.1 ATP-dependent DNA helicase RecG -
  FRD01_RS21370 (FRD01_21370) priA 5204727..5207144 (+) 2418 WP_249755809.1 primosomal protein N' -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40698.78 Da        Isoelectric Point: 7.5673

>NTDB_id=378038 FRD01_RS21315 WP_146962961.1 5192248..5193357(+) (pilT) [Microvenator marinus strain V1718]
MANLHQLLKIMVDQGSSDLHITVGAPPQLRIDGGLVPLRTAPLTPVDTQQLCYSVLTDAQKQHFEGEKELDLSFGVKGLS
RFRGNLFMQRGAVAGVFRLIPFEIKSFQELGLPQVVSAFADKPRGLVLVTGPTGSGKSTTLASIIDKINQEKRMHILTIE
DPIEYLHPHKNCLVNQREIGADTNGFKTALRYVLRQDPDVVLIGEMRDLETIEAALTISETGHLALATLHTNGCVQTINR
IVDVFPPHQQAQIRAQLSFVLEGIISQQLIPHASGRGRAMVMEVLVPNPAIRNLIREDKVHQIYSQMQVGQARSAMQTMN
QSLFNAYKKGDITLEDAMARSSDPEELRTMIEQGGQPNRGGSGGRVRYS

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=378038 FRD01_RS21315 WP_146962961.1 5192248..5193357(+) (pilT) [Microvenator marinus strain V1718]
GTGGCGAACCTTCATCAACTTCTGAAAATCATGGTCGATCAGGGGTCATCTGACCTCCACATTACCGTGGGTGCGCCCCC
GCAATTACGTATTGACGGTGGATTGGTTCCTTTGCGCACAGCGCCTTTGACGCCCGTTGATACCCAACAGCTTTGTTATA
GCGTGTTGACCGACGCGCAGAAGCAGCATTTTGAGGGCGAAAAGGAACTCGACCTTTCTTTTGGTGTGAAGGGCCTCAGC
CGATTCCGCGGCAACCTATTCATGCAGCGTGGTGCGGTCGCTGGCGTGTTCCGTCTGATTCCGTTTGAGATCAAGAGTTT
CCAAGAACTGGGGCTTCCCCAGGTTGTGAGCGCGTTTGCGGATAAGCCTCGCGGACTCGTACTGGTCACCGGCCCAACGG
GTTCGGGTAAGTCGACGACGCTTGCCAGTATCATCGATAAGATCAATCAAGAAAAGAGGATGCACATCCTCACCATTGAG
GATCCCATCGAGTATTTGCATCCGCACAAGAACTGCCTCGTGAATCAACGTGAGATCGGTGCGGATACCAACGGCTTCAA
GACGGCACTCAGGTACGTTCTGCGCCAGGATCCGGACGTTGTTCTGATCGGCGAGATGCGCGACCTCGAGACGATCGAAG
CTGCGCTTACGATTTCTGAAACGGGCCACCTTGCGTTGGCAACGCTTCACACCAACGGTTGTGTGCAGACCATCAACCGT
ATCGTGGACGTCTTCCCTCCGCACCAGCAGGCACAGATTCGAGCACAGCTCTCATTCGTGCTCGAAGGGATTATCTCCCA
GCAGCTCATCCCGCACGCCTCCGGGCGCGGCCGAGCGATGGTGATGGAGGTCCTCGTTCCGAATCCGGCCATTCGAAACC
TGATTCGCGAGGATAAAGTCCACCAAATTTATTCACAGATGCAGGTTGGTCAGGCTCGCTCAGCGATGCAGACCATGAAC
CAGTCGCTATTCAACGCGTACAAGAAGGGAGATATCACCCTTGAAGACGCAATGGCTCGAAGCAGCGACCCTGAAGAACT
TAGAACCATGATTGAACAGGGTGGACAACCCAACCGCGGCGGTTCCGGCGGTCGTGTCCGATACTCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8XXL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

93.496

0.485
  pilT Pseudomonas aeruginosa PAK

49.275

93.496

0.461
  pilT Legionella pneumophila strain Lp02

49.547

89.702

0.444
  pilT Legionella pneumophila strain ERS1305867

49.547

89.702

0.444
  pilT Acinetobacter baylyi ADP1

47.536

93.496

0.444
  pilT Pseudomonas stutzeri DSM 10701

47.536

93.496

0.444
  pilT Acinetobacter baumannii D1279779

46.957

93.496

0.439
  pilT Acinetobacter nosocomialis M2

46.957

93.496

0.439
  pilT Acinetobacter baumannii strain A118

46.957

93.496

0.439
  pilT Neisseria meningitidis 8013

46.334

92.412

0.428
  pilT Neisseria gonorrhoeae MS11

46.041

92.412

0.425
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.562

91.599

0.417
  pilT Vibrio cholerae strain A1552

45.562

91.599

0.417
  pilU Pseudomonas stutzeri DSM 10701

41.69

96.206

0.401
  pilU Vibrio cholerae strain A1552

42.12

94.58

0.398
  pilU Acinetobacter baylyi ADP1

39.612

97.832

0.388

Multiple sequence alignment