Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NWAT_RS14830 Genome accession   NC_014315
Coordinates   3246234..3247277 (+) Length   347 a.a.
NCBI ID   WP_013221842.1    Uniprot ID   D8KCB0
Organism   Nitrosococcus watsonii C-113     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3241234..3252277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWAT_RS14800 (Nwat_3054) pyrE 3241595..3242266 (-) 672 WP_013221836.1 orotate phosphoribosyltransferase -
  NWAT_RS14805 (Nwat_3055) - 3242423..3243619 (+) 1197 WP_013221837.1 AmpG family muropeptide MFS transporter -
  NWAT_RS14810 (Nwat_3056) - 3243631..3243939 (-) 309 WP_013221838.1 DUF167 domain-containing protein -
  NWAT_RS14815 (Nwat_3057) - 3243946..3244509 (-) 564 WP_013221839.1 YggT family protein -
  NWAT_RS14820 (Nwat_3058) proC 3244502..3245335 (-) 834 WP_013221840.1 pyrroline-5-carboxylate reductase -
  NWAT_RS14825 (Nwat_3059) - 3245432..3246127 (-) 696 WP_013221841.1 YggS family pyridoxal phosphate-dependent enzyme -
  NWAT_RS14830 (Nwat_3060) pilT 3246234..3247277 (+) 1044 WP_013221842.1 type IV pilus twitching motility protein PilT Machinery gene
  NWAT_RS14835 - 3247535..3247720 (-) 186 WP_041350506.1 hypothetical protein -
  NWAT_RS14840 - 3247722..3247976 (-) 255 WP_041350505.1 hypothetical protein -
  NWAT_RS14845 - 3247986..3248348 (-) 363 WP_041350503.1 hypothetical protein -
  NWAT_RS14850 - 3248829..3249122 (+) 294 WP_041350806.1 IS630 transposase-related protein -
  NWAT_RS14855 - 3249303..3249644 (+) 342 Protein_3004 transposase -
  NWAT_RS14860 (Nwat_3063) - 3250201..3250860 (+) 660 WP_013221843.1 hypothetical protein -
  NWAT_RS14865 (Nwat_3064) - 3251187..3251570 (+) 384 WP_013221844.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38489.11 Da        Isoelectric Point: 6.3496

>NTDB_id=37779 NWAT_RS14830 WP_013221842.1 3246234..3247277(+) (pilT) [Nitrosococcus watsonii C-113]
MDIAELLAFSVKNSASDLHISAGLPPMIRVDGDVRRINLPPMEHKDVHAMIYDIMNDKQRKDYEEFLETDFSFEIPGLAR
FRVNAFNQNRGAAAVFRTIPSQVLTLEELDTPAVFKDIADNPRGVVLVTGPTGSGKSTTLAAMVNYKNENDFAHILTIED
PIEFVHESKKSLINQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLLKKFGGGRVAAHEIMIGNPAIRNLIREDKIPQMYSAIQTGQGAGMQTLDQCL
SGLLRRNIVTKQEAAKKAVNKELFQSM

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=37779 NWAT_RS14830 WP_013221842.1 3246234..3247277(+) (pilT) [Nitrosococcus watsonii C-113]
ATGGATATCGCTGAACTGCTTGCCTTTAGTGTCAAGAATTCTGCCTCCGATTTACATATCTCGGCGGGACTCCCACCAAT
GATCCGGGTAGATGGCGACGTGCGCCGAATTAACTTACCGCCTATGGAACACAAGGATGTCCATGCCATGATCTACGATA
TCATGAATGACAAGCAGCGTAAGGATTATGAAGAATTTCTTGAGACCGACTTTTCCTTCGAAATCCCTGGATTAGCCCGT
TTCCGCGTGAATGCGTTCAATCAGAATCGTGGGGCGGCGGCGGTATTCCGCACCATTCCCTCCCAGGTGCTCACCCTTGA
AGAACTTGATACTCCCGCCGTATTTAAGGATATTGCCGATAATCCCCGGGGGGTGGTGCTAGTAACTGGACCTACCGGTT
CTGGCAAATCGACCACCTTAGCGGCCATGGTAAATTATAAAAACGAGAATGATTTTGCTCACATTTTAACCATCGAAGAT
CCCATCGAATTTGTCCACGAGAGTAAAAAAAGCCTAATTAATCAACGGGAGGTCCATCGAGATACCCACGGCTTTAGCGA
AGCGCTGCGTTCGGCATTGCGGGAAGACCCTGATATTATTCTGGTAGGTGAATTGCGAGATCTTGAAACTATTCGCCTCG
CATTGACGGCGGCAGAGACGGGTCATTTAGTCTTTGGCACCTTGCATACCAGTTCCGCAGCCAAGACTATTGATCGTATT
ATTGATGTATTCCCCGCTGCGGAGAAAGATATGGTGCGCTCTATGCTCTCCGAATCCCTGCGGGCCGTAATTTCTCAGAC
TCTCCTTAAAAAATTTGGCGGTGGCCGGGTTGCTGCCCACGAGATCATGATTGGCAATCCGGCTATTCGTAATCTCATCC
GTGAAGATAAGATCCCTCAAATGTATTCTGCCATCCAAACGGGCCAAGGGGCAGGTATGCAGACTCTGGATCAGTGCCTG
TCAGGCTTACTCCGTCGCAATATCGTCACTAAGCAGGAGGCAGCCAAGAAAGCCGTAAACAAAGAGCTTTTCCAGTCGAT
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D8KCB0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

80.233

99.135

0.795
  pilT Pseudomonas stutzeri DSM 10701

79.942

99.135

0.793
  pilT Acinetobacter nosocomialis M2

79.942

99.135

0.793
  pilT Acinetobacter baumannii D1279779

79.942

99.135

0.793
  pilT Acinetobacter baumannii strain A118

79.942

99.135

0.793
  pilT Acinetobacter baylyi ADP1

78.779

99.135

0.781
  pilT Legionella pneumophila strain Lp02

75.581

99.135

0.749
  pilT Legionella pneumophila strain ERS1305867

75.581

99.135

0.749
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.513

97.118

0.695
  pilT Vibrio cholerae strain A1552

71.513

97.118

0.695
  pilT Neisseria meningitidis 8013

69.388

98.847

0.686
  pilT Neisseria gonorrhoeae MS11

69.096

98.847

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

40.118

97.695

0.392
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392
  pilU Acinetobacter baylyi ADP1

38.529

97.983

0.378

Multiple sequence alignment