Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FS595_RS19250 Genome accession   NZ_CP042355
Coordinates   4059405..4060397 (-) Length   330 a.a.
NCBI ID   WP_054305193.1    Uniprot ID   A0A3S4GAE1
Organism   Serratia rubidaea strain H440     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4054405..4065397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FS595_RS19230 (FS595_19280) - 4054552..4054974 (-) 423 WP_232238940.1 DUF1090 domain-containing protein -
  FS595_RS19235 (FS595_19285) - 4055098..4058634 (-) 3537 WP_235081692.1 ATP-binding protein -
  FS595_RS23570 (FS595_19290) - 4058627..4059340 (-) 714 WP_276530873.1 response regulator transcription factor -
  FS595_RS19250 (FS595_19295) pilT 4059405..4060397 (-) 993 WP_054305193.1 type IV pilus twitching motility protein PilT Machinery gene
  FS595_RS19255 (FS595_19300) - 4060418..4061125 (+) 708 WP_235081693.1 YggS family pyridoxal phosphate-dependent enzyme -
  FS595_RS19260 (FS595_19305) proC 4061146..4061967 (+) 822 WP_054305191.1 pyrroline-5-carboxylate reductase -
  FS595_RS19265 (FS595_19310) - 4061987..4062541 (+) 555 WP_015673797.1 YggT family protein -
  FS595_RS19270 (FS595_19315) yggU 4062538..4062828 (+) 291 WP_015673798.1 DUF167 family protein YggU -
  FS595_RS19275 (FS595_19320) - 4062846..4063442 (+) 597 WP_015673799.1 XTP/dITP diphosphatase -
  FS595_RS19280 (FS595_19325) hemW 4063432..4064583 (+) 1152 WP_235081694.1 radical SAM family heme chaperone HemW -
  FS595_RS19285 (FS595_19330) - 4064624..4065058 (-) 435 WP_235081695.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35669.05 Da        Isoelectric Point: 7.0088

>NTDB_id=377366 FS595_RS19250 WP_054305193.1 4059405..4060397(-) (pilT) [Serratia rubidaea strain H440]
MDIDEFVALSVKHNASDLHLCTGQPLMMRIDGELQPLAGGAVLPAEAVQRLCAALLDEPQQRQLRRAGQLDCALTTAGGA
RLRANFFYQRAGVSLALRRIAECCPTLEQLQAPAIIPALTEREEGLILVTGATGSGKSTTLAAMIDHINRHQRRHILTLE
DPIEFIHHSALSLIQQRELGRDTRSFDAALSAALREDPDVILVGELRDSLSIRLALTAAETGHLVLATLHTRNAPQAVER
VIDVFPAEEKAYVRAQLASGLQAVIAQKLLRRAEGGRTAVFEILTATPAVSSMIREGKTHQLASVLQSGAQAGMQTFAQG
MQQRIAQGIL

Nucleotide


Download         Length: 993 bp        

>NTDB_id=377366 FS595_RS19250 WP_054305193.1 4059405..4060397(-) (pilT) [Serratia rubidaea strain H440]
ATGGATATCGATGAATTTGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTACCGGCCAACCGTTGAT
GATGCGCATCGATGGTGAACTTCAGCCGCTGGCGGGCGGCGCCGTCCTGCCCGCCGAGGCGGTGCAGCGGCTGTGCGCGG
CGCTGCTTGATGAGCCTCAGCAGCGGCAATTACGGCGCGCCGGGCAGCTGGATTGCGCATTGACAACCGCGGGCGGCGCG
CGGCTGCGCGCCAACTTTTTTTATCAGCGCGCCGGCGTGTCGCTGGCGCTGCGTCGTATTGCGGAATGCTGTCCGACGCT
GGAGCAGCTGCAGGCGCCGGCGATTATCCCGGCGCTCACCGAGCGTGAGGAGGGGCTGATTCTGGTCACGGGCGCGACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGATCGACCATATCAACCGTCATCAGCGGCGGCATATTCTGACGCTGGAA
GACCCGATTGAGTTTATCCACCACAGCGCGCTGTCGCTGATTCAGCAGCGCGAGCTGGGGCGCGACACCCGCAGCTTCGA
CGCGGCGCTGAGCGCCGCGCTGCGTGAAGATCCCGATGTGATCCTGGTCGGCGAGCTGCGCGACAGCCTCAGCATCCGGC
TGGCGCTGACCGCCGCGGAAACCGGCCATCTGGTGCTGGCGACTCTGCACACCCGCAACGCGCCGCAGGCGGTTGAGCGG
GTGATCGACGTCTTCCCGGCGGAGGAGAAAGCCTACGTGCGCGCCCAACTGGCCAGCGGTTTGCAGGCGGTCATCGCGCA
GAAATTATTGCGCAGGGCCGAAGGCGGCAGAACGGCGGTGTTTGAGATCCTGACGGCGACGCCGGCGGTAAGCAGCATGA
TCCGTGAAGGAAAAACGCATCAGCTGGCCAGCGTGTTGCAAAGCGGCGCGCAGGCGGGCATGCAAACCTTTGCTCAGGGA
ATGCAGCAACGTATTGCGCAGGGAATTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4GAE1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.727

100

0.527
  pilT Vibrio cholerae strain A1552

52.727

100

0.527
  pilT Acinetobacter baumannii D1279779

49.55

100

0.5
  pilT Acinetobacter baumannii strain A118

49.55

100

0.5
  pilT Acinetobacter baylyi ADP1

50

100

0.5
  pilT Acinetobacter nosocomialis M2

49.249

100

0.497
  pilT Neisseria gonorrhoeae MS11

48.802

100

0.494
  pilT Neisseria meningitidis 8013

48.503

100

0.491
  pilT Pseudomonas stutzeri DSM 10701

47.748

100

0.482
  pilT Legionella pneumophila strain ERS1305867

47.576

100

0.476
  pilT Legionella pneumophila strain Lp02

47.576

100

0.476
  pilT Pseudomonas aeruginosa PAK

46.847

100

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.799

96.364

0.461
  pilU Vibrio cholerae strain A1552

41.358

98.182

0.406
  pilU Pseudomonas stutzeri DSM 10701

39.818

99.697

0.397
  pilU Acinetobacter baylyi ADP1

39.319

97.879

0.385

Multiple sequence alignment