Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HAV35_RS11720 Genome accession   NZ_CP050120
Coordinates   2333898..2334977 (+) Length   359 a.a.
NCBI ID   WP_010887087.1    Uniprot ID   Q9RX73
Organism   Deinococcus radiodurans strain BND-54     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2328898..2339977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAV35_RS11690 (HAV35_11695) - 2329198..2329407 (-) 210 WP_010887093.1 helix-turn-helix transcriptional regulator -
  HAV35_RS11695 (HAV35_11700) pyrE 2329455..2330039 (+) 585 WP_010887092.1 orotate phosphoribosyltransferase -
  HAV35_RS11700 (HAV35_11705) - 2330079..2330390 (+) 312 WP_010887091.1 antibiotic biosynthesis monooxygenase -
  HAV35_RS11705 (HAV35_11710) - 2330391..2331035 (-) 645 WP_034349494.1 DUF2268 domain-containing protein -
  HAV35_RS11710 (HAV35_11715) - 2331086..2332273 (-) 1188 WP_010887089.1 glycosyltransferase family 4 protein -
  HAV35_RS11715 (HAV35_11720) - 2332359..2333684 (+) 1326 WP_034349496.1 thymidine phosphorylase -
  HAV35_RS11720 (HAV35_11725) pilT 2333898..2334977 (+) 1080 WP_010887087.1 type IV pilus twitching motility protein PilT Machinery gene
  HAV35_RS11725 (HAV35_11730) - 2334993..2335703 (-) 711 WP_027479560.1 PrsW family glutamic-type intramembrane protease -
  HAV35_RS11730 (HAV35_11735) ruvC 2335714..2336253 (-) 540 WP_010887085.1 crossover junction endodeoxyribonuclease RuvC -
  HAV35_RS11735 (HAV35_11740) - 2336352..2336771 (-) 420 WP_010887084.1 iron-sulfur cluster assembly accessory protein -
  HAV35_RS11740 (HAV35_11745) - 2336923..2337201 (+) 279 WP_027479561.1 hypothetical protein -
  HAV35_RS11745 (HAV35_11750) - 2337264..2337482 (-) 219 WP_010887082.1 hypothetical protein -
  HAV35_RS11750 (HAV35_11755) - 2337551..2338888 (-) 1338 WP_010887081.1 cytochrome bc complex cytochrome b subunit -
  HAV35_RS11755 (HAV35_11760) - 2338885..2339547 (-) 663 WP_027479563.1 ubiquinol-cytochrome c reductase iron-sulfur subunit -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39675.59 Da        Isoelectric Point: 6.2435

>NTDB_id=376843 HAV35_RS11720 WP_010887087.1 2333898..2334977(+) (pilT) [Deinococcus radiodurans strain BND-54]
MSVLNQLLAALVKEGASDIHLRAGTAPAARINGVIRRFGESRLSPQHLQQFTQEMLPSTALWESFNARREADFAYGLAGV
GRFRVNAYFQRGSIGLIMRVIEDKPIPTFEELGLPTAVFNELAEQERGLVLVTGPTGSGKTTTLASLIDYINSHQPVNIV
TLEDPIEVLHRDKMAMVNQRELGLDTLSFSNGLRAAMRQDPDIILIGEMRDKETVEAALSAAQTGHLVLSTLHTQDAIRS
VNRIIDFFAPHERTQIRQGLSESMVGIVSQRLLPRSGGGRVMGMEVMLGTPTVRECIKDPERTEEIKQALMEGGMRGMRT
FDQHLVQLVLDGQMTQEDAMSAATSPHELKLMLMKSQYA

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=376843 HAV35_RS11720 WP_010887087.1 2333898..2334977(+) (pilT) [Deinococcus radiodurans strain BND-54]
ATGAGCGTTCTGAACCAGCTGCTCGCCGCCCTGGTCAAGGAAGGTGCGAGCGATATTCACCTGCGGGCCGGCACCGCGCC
GGCTGCCCGCATCAACGGGGTGATTCGGCGCTTCGGCGAGAGCCGTCTGTCTCCCCAGCACCTGCAACAGTTCACGCAGG
AAATGCTGCCCAGCACGGCGCTGTGGGAAAGCTTCAACGCCCGGCGCGAGGCCGACTTCGCCTACGGGCTGGCGGGCGTG
GGGCGCTTTCGCGTCAACGCCTATTTTCAGCGCGGGTCCATCGGCCTGATTATGCGCGTCATTGAGGACAAGCCGATTCC
GACCTTCGAGGAACTGGGCCTGCCCACCGCCGTCTTCAACGAACTCGCCGAGCAGGAGCGCGGGCTGGTGCTGGTCACTG
GCCCCACCGGCAGCGGCAAGACGACCACCCTGGCGAGCCTGATCGATTACATCAACAGCCATCAGCCGGTCAACATCGTG
ACGCTCGAAGACCCCATCGAGGTGCTGCACCGCGACAAGATGGCGATGGTCAACCAACGCGAGCTCGGGCTCGACACCCT
CAGCTTCTCCAACGGGCTGCGGGCGGCCATGCGGCAAGACCCCGACATCATCCTCATCGGCGAGATGCGCGACAAGGAAA
CGGTGGAAGCGGCCCTCTCGGCGGCGCAGACCGGGCACCTCGTGCTCAGCACGCTGCACACCCAGGACGCGATTCGCTCG
GTCAACCGCATCATCGATTTCTTCGCGCCGCACGAACGCACCCAGATTCGCCAGGGCCTCTCCGAGAGCATGGTGGGTAT
TGTCAGCCAGCGTCTCTTGCCGCGGAGTGGCGGGGGCCGGGTCATGGGTATGGAAGTCATGCTCGGCACGCCCACCGTCC
GCGAGTGCATCAAAGACCCCGAACGCACCGAGGAAATCAAACAGGCCCTGATGGAAGGTGGCATGCGCGGGATGCGGACC
TTCGACCAGCACCTCGTGCAACTGGTGCTGGACGGCCAGATGACGCAGGAAGACGCAATGAGCGCCGCCACCAGCCCCCA
CGAACTCAAGCTGATGCTGATGAAGTCGCAGTACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9RX73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

43.931

96.379

0.423
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.804

96.657

0.423
  pilT Acinetobacter nosocomialis M2

43.149

95.543

0.412
  pilT Acinetobacter baumannii D1279779

43.149

95.543

0.412
  pilT Acinetobacter baumannii strain A118

43.149

95.543

0.412
  pilT Legionella pneumophila strain ERS1305867

43.787

94.15

0.412
  pilT Legionella pneumophila strain Lp02

43.787

94.15

0.412
  pilU Vibrio cholerae strain A1552

42.165

97.772

0.412
  pilT Pseudomonas stutzeri DSM 10701

42.486

96.379

0.409
  pilT Acinetobacter baylyi ADP1

42.566

95.543

0.407
  pilT Neisseria meningitidis 8013

43.323

93.872

0.407
  pilT Neisseria gonorrhoeae MS11

43.323

93.872

0.407
  pilT Vibrio cholerae strain A1552

42.857

93.593

0.401
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.857

93.593

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.115

97.214

0.39
  pilU Acinetobacter baylyi ADP1

37.709

99.721

0.376

Multiple sequence alignment