Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HAV35_RS08040 Genome accession   NZ_CP050120
Coordinates   1595278..1596351 (+) Length   357 a.a.
NCBI ID   WP_010887806.1    Uniprot ID   Q9RV67
Organism   Deinococcus radiodurans strain BND-54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1590278..1601351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAV35_RS08010 (HAV35_08015) - 1590686..1591843 (+) 1158 WP_166466098.1 VWA-like domain-containing protein -
  HAV35_RS08015 (HAV35_08020) nudC 1591840..1592682 (+) 843 WP_010887811.1 NAD(+) diphosphatase -
  HAV35_RS08020 (HAV35_08025) - 1592651..1593253 (-) 603 WP_010887810.1 DedA family protein -
  HAV35_RS08025 (HAV35_08030) - 1593286..1593870 (-) 585 WP_010887809.1 hypothetical protein -
  HAV35_RS08030 (HAV35_08035) - 1594012..1594383 (+) 372 WP_010887808.1 phage holin family protein -
  HAV35_RS08035 (HAV35_08040) panC 1594436..1595281 (+) 846 WP_010887807.1 pantoate--beta-alanine ligase -
  HAV35_RS08040 (HAV35_08045) pilT 1595278..1596351 (+) 1074 WP_010887806.1 type IV pilus twitching motility protein PilT Machinery gene
  HAV35_RS08045 (HAV35_08050) greA 1596400..1596876 (+) 477 WP_010887805.1 transcription elongation factor GreA -
  HAV35_RS08050 (HAV35_08055) uraH 1596987..1597346 (-) 360 WP_010887804.1 hydroxyisourate hydrolase -
  HAV35_RS16380 pucL 1597343..1597846 (-) 504 Protein_1599 factor-independent urate hydroxylase -
  HAV35_RS16385 - 1597771..1598238 (-) 468 WP_341765337.1 hypothetical protein -
  HAV35_RS08060 (HAV35_08065) - 1598459..1598884 (+) 426 WP_243759708.1 MarR family transcriptional regulator -
  HAV35_RS08065 (HAV35_08070) uraD 1599021..1599587 (-) 567 WP_010887801.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
  HAV35_RS08070 (HAV35_08075) - 1599619..1599846 (+) 228 WP_156504619.1 hypothetical protein -
  HAV35_RS08075 (HAV35_08080) - 1599852..1601084 (+) 1233 WP_027479609.1 glycerate kinase -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39412.17 Da        Isoelectric Point: 6.2347

>NTDB_id=376825 HAV35_RS08040 WP_010887806.1 1595278..1596351(+) (pilT) [Deinococcus radiodurans strain BND-54]
MTLDDLLRELAGRRASDIHLQAGSPPLGRVDGRLLPFGTQPLTPEHTQALARAVLTPEQFEEFEYQQELDVAYSVPGLGR
FRCNVFRQRGAVGLVLRVVGETIPGFEALGLPREVMEQFAALPRGLVLITGPTGSGKTTTVASLIDYLNRHFAYHILTIE
DPIEVLHRNNRSLVVQREVGRDTRSFAAALRHALRQDPDVIVIGEIRDRETVEAALLAAQTGHLVISTLHTQDAVRTVGR
IAEFFAPHERDQVRLQLAECLGGILSQRLLPRADGVGRALATEVLVSTPLVQEYVKDEARTPLLKDVLLEDNLRGMHTFD
EHLAALYRAELITLDAALAAATSPHELRLLLTQTGRL

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=376825 HAV35_RS08040 WP_010887806.1 1595278..1596351(+) (pilT) [Deinococcus radiodurans strain BND-54]
ATGACGCTGGACGACCTGCTGCGCGAACTCGCGGGCCGCCGCGCCTCCGACATCCACCTGCAAGCGGGCAGTCCGCCGCT
GGGCCGGGTGGATGGCCGCTTGCTGCCCTTTGGCACGCAACCACTGACGCCGGAGCATACCCAGGCGCTCGCACGGGCGG
TGCTGACCCCTGAGCAGTTTGAGGAATTCGAGTACCAGCAGGAACTGGACGTGGCCTACAGCGTGCCGGGTCTGGGCCGC
TTCCGCTGCAACGTGTTCCGGCAGCGCGGTGCGGTGGGCCTGGTGCTGCGGGTGGTGGGCGAAACCATTCCGGGCTTCGA
GGCACTCGGACTACCGCGCGAAGTCATGGAGCAGTTCGCGGCGCTGCCCCGTGGCCTGGTGCTGATTACCGGGCCGACCG
GCAGTGGCAAGACCACCACCGTCGCCAGCCTCATCGACTACCTCAACCGGCACTTCGCCTATCACATTCTGACCATCGAA
GACCCCATCGAGGTGCTGCACCGCAACAACCGCAGTCTGGTGGTACAGCGCGAGGTGGGGCGCGACACTCGCAGTTTCGC
GGCGGCGCTGCGGCACGCGCTGCGTCAAGACCCCGACGTGATCGTGATCGGCGAGATTCGGGACCGCGAAACGGTGGAGG
CCGCGCTGCTGGCGGCGCAGACCGGGCATCTGGTCATCAGCACGCTGCACACCCAGGACGCGGTGCGGACGGTAGGGCGC
ATCGCCGAATTTTTCGCCCCCCACGAGCGCGATCAGGTGCGGCTGCAACTCGCCGAGTGCCTGGGCGGCATCCTCAGCCA
GCGGCTGCTGCCCCGCGCCGACGGGGTGGGCCGGGCACTGGCGACCGAGGTGCTCGTCAGCACGCCCCTGGTGCAGGAAT
ATGTCAAGGACGAGGCGCGCACGCCGCTGCTCAAGGACGTGCTGCTCGAAGACAATCTGCGGGGGATGCACACCTTCGAC
GAGCACCTGGCCGCGCTCTACCGTGCCGAGCTGATCACCCTCGACGCGGCGCTCGCGGCGGCCACCAGCCCGCACGAACT
GCGGCTGCTGCTGACCCAGACCGGGCGACTCTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9RV67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.667

96.639

0.451
  pilT Pseudomonas aeruginosa PAK

42.075

97.199

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.595

98.319

0.409
  pilT Pseudomonas stutzeri DSM 10701

41.787

97.199

0.406
  pilT Acinetobacter baylyi ADP1

41.499

97.199

0.403
  pilU Vibrio cholerae strain A1552

41.026

98.319

0.403
  pilT Neisseria meningitidis 8013

41.246

94.398

0.389
  pilT Neisseria gonorrhoeae MS11

41.246

94.398

0.389
  pilT Legionella pneumophila strain Lp02

40.762

95.518

0.389
  pilT Legionella pneumophila strain ERS1305867

40.762

95.518

0.389
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.025

91.317

0.384
  pilT Vibrio cholerae strain A1552

42.025

91.317

0.384
  pilU Acinetobacter baylyi ADP1

39.143

98.039

0.384
  pilT Acinetobacter baumannii D1279779

39.481

97.199

0.384
  pilT Acinetobacter baumannii strain A118

39.481

97.199

0.384
  pilT Acinetobacter nosocomialis M2

39.481

97.199

0.384

Multiple sequence alignment