Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BSEL_RS07450 Genome accession   NC_014219
Coordinates   1640780..1641811 (+) Length   343 a.a.
NCBI ID   WP_013172376.1    Uniprot ID   D6XT16
Organism   [Bacillus] selenitireducens MLS10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1635780..1646811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSEL_RS17865 (Bsel_1437) - 1636597..1637658 (+) 1062 WP_013172373.1 hypothetical protein -
  BSEL_RS07440 (Bsel_1438) - 1637552..1639036 (+) 1485 WP_177304818.1 VanW family protein -
  BSEL_RS07445 (Bsel_1439) - 1639070..1640737 (+) 1668 WP_013172375.1 GspE/PulE family protein -
  BSEL_RS07450 (Bsel_1440) pilT 1640780..1641811 (+) 1032 WP_013172376.1 type IV pilus twitching motility protein PilT Machinery gene
  BSEL_RS07455 (Bsel_1441) - 1641871..1643079 (+) 1209 WP_013172377.1 type II secretion system F family protein -
  BSEL_RS17490 (Bsel_1442) - 1643452..1643934 (+) 483 WP_013172378.1 type II secretion system protein -
  BSEL_RS18015 (Bsel_1443) - 1644045..1644515 (+) 471 WP_013172379.1 Tfp pilus assembly protein FimT/FimU -
  BSEL_RS07470 (Bsel_1444) - 1644672..1645433 (+) 762 WP_013172380.1 A24 family peptidase -
  BSEL_RS07475 (Bsel_1445) pilM 1645588..1646571 (+) 984 WP_013172381.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37919.70 Da        Isoelectric Point: 6.7057

>NTDB_id=37485 BSEL_RS07450 WP_013172376.1 1640780..1641811(+) (pilT) [[Bacillus] selenitireducens MLS10]
MKERIEELLRKAFYDGASDLHITVGVPPVMRTNGELEKVGTDMMKPADTEGMARAIIPEALWKNFEKKGELDFSYGLRGV
SRFRVNAYFQRSCVSLAIRVIPTVIPTIDDLNMPKILESIASKPQGLVLVTGPTGSGKSTTLAAMISYMNTRMNRHIITL
EDPIEYLHKHGTSVIDQREIGFDTQNFANGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVLGTLHTTNAPSTID
RIIDVFPPGQQDQIRIQVASVLTSVISQRLLRTRDGGGRVAATEILMNTPAVKNLIRNEKIHQIPSTMQTGKAEGMHTME
MAVKDLLQERKISEETAAYILSE

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=37485 BSEL_RS07450 WP_013172376.1 1640780..1641811(+) (pilT) [[Bacillus] selenitireducens MLS10]
GTGAAAGAACGGATTGAAGAGCTACTCAGAAAGGCATTCTATGATGGTGCGTCAGATTTGCATATTACCGTGGGTGTGCC
CCCGGTCATGCGGACGAACGGTGAACTTGAGAAGGTCGGGACCGACATGATGAAACCGGCGGACACGGAAGGGATGGCCC
GAGCGATCATTCCGGAAGCGCTCTGGAAGAACTTTGAGAAGAAGGGGGAGCTCGACTTCTCCTATGGCCTTCGGGGCGTA
TCCCGCTTTCGGGTGAACGCGTACTTTCAGCGCTCCTGCGTGAGTCTTGCGATCCGCGTGATTCCGACAGTGATTCCGAC
GATTGACGATTTGAATATGCCGAAGATCCTGGAATCCATCGCCTCAAAGCCCCAGGGGCTCGTCCTTGTGACCGGACCGA
CGGGCTCCGGGAAATCGACGACCCTGGCAGCAATGATCTCATACATGAACACAAGGATGAACCGGCACATCATCACCCTT
GAAGATCCGATTGAGTATTTGCATAAGCACGGCACGTCGGTGATTGACCAGCGCGAGATCGGCTTTGACACGCAGAACTT
CGCCAACGGCCTCCGGGCGGCGCTCCGTCAGGATCCGGATGTGATTCTCGTCGGGGAGATGCGGGATCTTGAGACGATCT
CCACGGCGATTACCGCGGCGGAAACCGGACACCTCGTCCTCGGTACGCTTCATACGACGAACGCGCCGTCAACGATTGAC
CGGATCATTGACGTCTTCCCCCCGGGCCAGCAGGATCAGATCCGCATACAGGTGGCCTCTGTCCTCACGTCGGTCATCTC
GCAGCGTCTTCTTCGCACGAGAGACGGCGGCGGCCGGGTGGCGGCGACGGAGATTCTCATGAATACGCCGGCGGTGAAGA
ACCTGATCCGAAACGAAAAGATTCACCAGATCCCGTCCACGATGCAGACCGGTAAGGCAGAAGGGATGCACACAATGGAG
ATGGCCGTGAAGGATCTCCTGCAGGAGCGCAAGATCAGTGAAGAAACGGCAGCTTATATCCTGTCGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D6XT16

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

53.87

94.169

0.507
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.87

94.169

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.28

95.918

0.501
  pilT Neisseria meningitidis 8013

49.401

97.376

0.481
  pilT Neisseria gonorrhoeae MS11

49.102

97.376

0.478
  pilT Pseudomonas stutzeri DSM 10701

48.949

97.085

0.475
  pilT Acinetobacter nosocomialis M2

48.949

97.085

0.475
  pilT Acinetobacter baumannii D1279779

48.949

97.085

0.475
  pilT Acinetobacter baumannii strain A118

48.949

97.085

0.475
  pilT Acinetobacter baylyi ADP1

48.649

97.085

0.472
  pilT Pseudomonas aeruginosa PAK

48.348

97.085

0.469
  pilT Legionella pneumophila strain ERS1305867

47.748

97.085

0.464
  pilT Legionella pneumophila strain Lp02

47.748

97.085

0.464
  pilU Pseudomonas stutzeri DSM 10701

42.943

97.085

0.417
  pilU Vibrio cholerae strain A1552

42.415

94.169

0.399
  pilU Acinetobacter baylyi ADP1

41.824

92.711

0.388

Multiple sequence alignment