Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DAAHT2_RS05450 Genome accession   NC_014216
Coordinates   1279219..1280301 (-) Length   360 a.a.
NCBI ID   WP_013163295.1    Uniprot ID   -
Organism   Desulfurivibrio alkaliphilus AHT     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1274219..1285301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAAHT2_RS05440 (DaAHT2_1066) - 1275209..1276546 (+) 1338 WP_013163293.1 sigma-70 family RNA polymerase sigma factor -
  DAAHT2_RS05445 (DaAHT2_1067) - 1276560..1279124 (+) 2565 WP_013163294.1 DNA internalization-related competence protein ComEC/Rec2 -
  DAAHT2_RS05450 (DaAHT2_1068) pilT 1279219..1280301 (-) 1083 WP_013163295.1 type IV pilus twitching motility protein PilT Machinery gene
  DAAHT2_RS05455 (DaAHT2_1069) - 1280544..1281449 (+) 906 WP_013163296.1 PHP domain-containing protein -
  DAAHT2_RS05460 (DaAHT2_1070) - 1281446..1282831 (+) 1386 WP_013163297.1 sigma-54 dependent transcriptional regulator -
  DAAHT2_RS05465 (DaAHT2_1071) - 1282954..1283334 (+) 381 WP_041718829.1 DUF948 domain-containing protein -
  DAAHT2_RS05470 (DaAHT2_1072) - 1283368..1283760 (+) 393 WP_013163299.1 YtxH domain-containing protein -
  DAAHT2_RS05475 (DaAHT2_1073) - 1283764..1284597 (-) 834 WP_013163300.1 NAD(+)/NADH kinase -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39737.91 Da        Isoelectric Point: 6.5026

>NTDB_id=37448 DAAHT2_RS05450 WP_013163295.1 1279219..1280301(-) (pilT) [Desulfurivibrio alkaliphilus AHT]
MAQIDAFFKLMNDQGASDLHMVAGQQPVLRIRGDMEKVKYNVMENDALKSMLYEIVSEEKIKVFEETGDIDFGYEITGLA
RYRGNLFMQKNGIGAVFREIPSEILSCEQLGLPAVVSRLATLPKGLVLVTGPTGSGKSTTLAAIIDQANKTRKDHILTIE
DPIEFVHKSHNCIVNHREVGLHTQSFSAALRGALREDPDIILVGEMRDLETIALAMEAAMTGHLVFGTLHTLNANKTVDR
IIEIFPASQQAQVRSTLADALKAVVSQNLFKRIDKKGRCAALEILICTPAVRNLIREGKTYQIPSAMQTGKKFGMQTLDD
AIMDVLQKGWISGEDAYVNAIEKSKFVQYLKTPPADFTDA

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=37448 DAAHT2_RS05450 WP_013163295.1 1279219..1280301(-) (pilT) [Desulfurivibrio alkaliphilus AHT]
ATGGCTCAGATCGATGCATTTTTTAAATTGATGAACGACCAGGGGGCTTCCGACCTTCACATGGTAGCGGGTCAGCAACC
GGTACTGCGGATTCGCGGGGATATGGAGAAGGTTAAATATAATGTGATGGAAAACGATGCGCTAAAGTCGATGCTCTACG
AGATCGTTTCGGAAGAAAAGATTAAGGTATTCGAGGAAACCGGCGATATCGATTTTGGTTACGAAATAACGGGGCTGGCC
CGTTACCGGGGTAACCTTTTTATGCAAAAAAACGGGATCGGCGCTGTTTTTCGGGAGATCCCCAGTGAGATTCTAAGCTG
TGAGCAGTTGGGGTTGCCGGCCGTGGTTTCTCGCCTGGCCACTCTGCCCAAGGGGCTGGTGCTGGTTACCGGTCCCACCG
GCAGCGGTAAATCGACGACCCTGGCGGCCATTATTGATCAGGCCAATAAGACCCGCAAGGACCATATCCTTACCATCGAA
GACCCCATAGAATTTGTGCACAAGAGCCACAATTGCATCGTCAACCACCGTGAGGTGGGCCTGCATACCCAGAGCTTTTC
CGCCGCCCTGCGCGGTGCCCTGCGCGAGGATCCGGACATTATTCTGGTGGGCGAAATGCGGGATCTGGAAACCATCGCGC
TGGCCATGGAGGCGGCCATGACCGGGCACCTGGTTTTTGGCACCCTGCACACCCTGAACGCCAACAAGACCGTGGATCGG
ATCATCGAAATTTTTCCTGCCAGTCAGCAGGCCCAGGTCCGTTCTACCCTGGCCGACGCGCTGAAAGCGGTGGTTTCCCA
GAACCTCTTTAAACGCATCGATAAAAAAGGGCGCTGTGCCGCCCTGGAGATCCTGATCTGCACCCCGGCGGTGCGCAACC
TGATCCGGGAAGGCAAGACCTACCAGATTCCCTCGGCCATGCAGACCGGCAAAAAATTCGGCATGCAAACCCTGGATGAC
GCCATTATGGATGTATTGCAAAAAGGCTGGATTTCCGGGGAAGATGCCTATGTCAACGCCATCGAGAAAAGCAAGTTCGT
GCAGTACCTGAAAACCCCGCCGGCGGATTTCACCGACGCCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

56.627

92.222

0.522
  pilT Legionella pneumophila strain Lp02

56.495

91.944

0.519
  pilT Legionella pneumophila strain ERS1305867

56.495

91.944

0.519
  pilT Pseudomonas stutzeri DSM 10701

54.655

92.5

0.506
  pilT Acinetobacter baumannii strain A118

51.497

92.778

0.478
  pilT Acinetobacter nosocomialis M2

51.497

92.778

0.478
  pilT Acinetobacter baumannii D1279779

51.497

92.778

0.478
  pilT Acinetobacter baylyi ADP1

53.943

88.056

0.475
  pilT Neisseria meningitidis 8013

52.96

89.167

0.472
  pilT Neisseria gonorrhoeae MS11

52.648

89.167

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.536

95.833

0.456
  pilT Vibrio cholerae strain A1552

50

89.444

0.447
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

89.444

0.447
  pilU Pseudomonas stutzeri DSM 10701

41.716

93.889

0.392
  pilU Acinetobacter baylyi ADP1

40.299

93.056

0.375
  pilU Vibrio cholerae strain A1552

37.288

98.333

0.367

Multiple sequence alignment