Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FO014_RS14370 Genome accession   NZ_CP041764
Coordinates   3079028..3080020 (+) Length   330 a.a.
NCBI ID   WP_160030032.1    Uniprot ID   -
Organism   Serratia rhizosphaerae strain KUDC3025     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3074028..3085020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FO014_RS14340 (FO014_14460) hemW 3074842..3075993 (-) 1152 WP_160030028.1 radical SAM family heme chaperone HemW -
  FO014_RS14345 (FO014_14465) - 3075983..3076579 (-) 597 WP_160030029.1 XTP/dITP diphosphatase -
  FO014_RS14350 (FO014_14470) yggU 3076597..3076887 (-) 291 WP_105232509.1 DUF167 family protein YggU -
  FO014_RS14355 (FO014_14475) - 3076884..3077438 (-) 555 WP_015673797.1 YggT family protein -
  FO014_RS14360 (FO014_14480) proC 3077458..3078279 (-) 822 WP_160030030.1 pyrroline-5-carboxylate reductase -
  FO014_RS14365 (FO014_14485) - 3078300..3079007 (-) 708 WP_160030031.1 YggS family pyridoxal phosphate-dependent enzyme -
  FO014_RS14370 (FO014_14490) pilT 3079028..3080020 (+) 993 WP_160030032.1 type IV pilus twitching motility protein PilT Machinery gene
  FO014_RS24170 (FO014_14495) - 3080165..3080797 (+) 633 WP_160030033.1 response regulator transcription factor -
  FO014_RS14380 (FO014_14500) - 3080790..3084332 (+) 3543 WP_160030034.1 transporter substrate-binding domain-containing protein -
  FO014_RS14385 (FO014_14505) - 3084440..3084883 (+) 444 WP_160030035.1 DUF1090 domain-containing protein -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35838.10 Da        Isoelectric Point: 7.2383

>NTDB_id=373931 FO014_RS14370 WP_160030032.1 3079028..3080020(+) (pilT) [Serratia rhizosphaerae strain KUDC3025]
MDIDEFVALSVKHNASDLHLCTGQPLMMRIDGELQPLAGDAALSAADMQPLCTALLDERQQRQLQRAGQLDCALTTTSGT
RLRANFFYQHAGISLALRRIAERCPTLEQLQAPAIIPTLTEREEGLILVTGATGSGKSTTLAAMIDHINRHQRRHILTLE
DPIEFIHRSALSLIQQRQLGRDTRSFDAALSAALREDPDVILVGELRDGASIRLALTAAETGHLVLATLHTRNAPQAVER
MIDVFPAEEKAYVRAQLASGLQAVIAQKLLRRADGGRTAVFEILTATPAVSSMIREGKTHQLASVLQSGAQAGMQTFAQG
KQQRIAQGIL

Nucleotide


Download         Length: 993 bp        

>NTDB_id=373931 FO014_RS14370 WP_160030032.1 3079028..3080020(+) (pilT) [Serratia rhizosphaerae strain KUDC3025]
ATGGATATCGATGAATTTGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTACCGGCCAACCGTTGAT
GATGCGCATCGATGGTGAACTTCAGCCGCTGGCGGGCGACGCCGCCCTGTCTGCTGCAGACATGCAACCGCTGTGTACCG
CGCTGCTCGACGAGCGCCAGCAGCGGCAGCTGCAGCGGGCCGGCCAGCTGGATTGCGCCTTGACGACCACGAGCGGCACG
CGGCTGCGCGCCAACTTTTTTTATCAGCACGCCGGCATATCGCTGGCGCTGCGTCGCATTGCGGAACGTTGCCCGACGTT
GGAGCAGCTGCAGGCGCCGGCGATTATACCGACGCTCACCGAGCGGGAAGAGGGGCTGATTCTGGTGACGGGAGCGACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACCATATCAACCGTCACCAGCGGCGGCATATCCTGACGCTGGAA
GATCCGATCGAGTTTATCCACCGCAGCGCGCTGTCGCTGATTCAGCAGCGACAGTTGGGCCGCGATACCCGCAGCTTCGA
TGCGGCGCTGAGCGCCGCGCTGCGTGAAGATCCTGACGTTATTCTGGTGGGTGAGCTGCGCGACGGCGCCAGCATCCGCC
TGGCGCTGACCGCGGCGGAAACCGGCCATCTGGTGCTGGCGACGTTGCATACCCGCAACGCCCCTCAGGCGGTTGAGCGG
ATGATCGACGTCTTTCCGGCGGAAGAGAAAGCCTATGTGCGCGCCCAATTGGCCAGCGGTTTGCAGGCGGTAATCGCGCA
GAAATTACTGCGCAGAGCCGACGGCGGCAGAACGGCTGTATTTGAAATCCTGACGGCGACGCCGGCGGTAAGCAGCATGA
TTCGCGAAGGAAAAACGCATCAGCTGGCCAGCGTGCTGCAAAGCGGGGCGCAGGCGGGTATGCAAACCTTTGCTCAGGGC
AAACAGCAGCGTATTGCGCAGGGAATTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

52.424

100

0.524
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.424

100

0.524
  pilT Acinetobacter baylyi ADP1

48.788

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.64

100

0.488
  pilT Acinetobacter baumannii D1279779

48.485

100

0.485
  pilT Acinetobacter baumannii strain A118

48.485

100

0.485
  pilT Neisseria meningitidis 8013

48.338

100

0.485
  pilT Acinetobacter nosocomialis M2

48.182

100

0.482
  pilT Legionella pneumophila strain ERS1305867

46.97

100

0.47
  pilT Legionella pneumophila strain Lp02

46.97

100

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.95

96.061

0.461
  pilT Pseudomonas stutzeri DSM 10701

46.061

100

0.461
  pilT Pseudomonas aeruginosa PAK

45.455

100

0.455
  pilU Vibrio cholerae strain A1552

41.718

98.788

0.412
  pilU Pseudomonas stutzeri DSM 10701

38.906

99.697

0.388
  pilU Acinetobacter baylyi ADP1

39.13

97.576

0.382

Multiple sequence alignment