Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FOH38_RS16145 Genome accession   NZ_CP041696
Coordinates   3327809..3328855 (+) Length   348 a.a.
NCBI ID   WP_143997813.1    Uniprot ID   -
Organism   Lysinibacillus fusiformis strain 1226     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3322809..3333855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOH38_RS16125 (FOH38_16125) - 3323458..3323922 (-) 465 WP_143997809.1 PilW family protein -
  FOH38_RS16130 (FOH38_16130) - 3323922..3324401 (-) 480 WP_369435920.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FOH38_RS16135 (FOH38_16135) - 3324774..3326108 (+) 1335 WP_143997811.1 VanW family protein -
  FOH38_RS16140 (FOH38_16140) - 3326127..3327797 (+) 1671 WP_143997812.1 GspE/PulE family protein -
  FOH38_RS16145 (FOH38_16145) pilT 3327809..3328855 (+) 1047 WP_143997813.1 type IV pilus twitching motility protein PilT Machinery gene
  FOH38_RS16150 (FOH38_16150) - 3328852..3330057 (+) 1206 WP_143997814.1 type II secretion system F family protein -
  FOH38_RS16155 (FOH38_16155) - 3330314..3330805 (+) 492 WP_143997815.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FOH38_RS16160 (FOH38_16160) pilM 3330952..3331926 (+) 975 WP_143997816.1 type IV pilus biogenesis protein PilM -
  FOH38_RS16165 (FOH38_16165) - 3331926..3332519 (+) 594 WP_143997817.1 PilN domain-containing protein -
  FOH38_RS16170 (FOH38_16170) - 3332516..3333202 (+) 687 WP_143997818.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38931.66 Da        Isoelectric Point: 6.8736

>NTDB_id=373276 FOH38_RS16145 WP_143997813.1 3327809..3328855(+) (pilT) [Lysinibacillus fusiformis strain 1226]
MTAYSIVELLTRSFEEKASDLHLTKGIPPVYRMHGKLEHYGTTPLETEDLQEMVKVILPAHKQQEFEDKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAKIPSIETLNMPEVLYRLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETRSKHIIT
LEDPIEYLHSHKKSVINQREVGVDTMSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQSQIRVQLANVLQGIISQRLFVRKDVKGRIAATEILVQAPSISNLIRNEKIHQIPSVMQTSRALGMHTL
ESSMQHLIATGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=373276 FOH38_RS16145 WP_143997813.1 3327809..3328855(+) (pilT) [Lysinibacillus fusiformis strain 1226]
ATGACAGCATATTCAATTGTAGAGCTATTAACTCGCTCATTTGAGGAAAAGGCATCAGATTTACATTTAACAAAAGGCAT
CCCCCCTGTTTATCGTATGCATGGCAAGTTAGAGCATTATGGCACTACACCACTTGAAACAGAAGATTTACAGGAAATGG
TGAAGGTTATTTTACCAGCACATAAACAACAGGAATTTGAGGATAAAGGCGAGACGGACTTTAACTTTTCATTGCCTGGA
AAATGCCGGTTCCGTGTCAATGCCTACCATCAACGCAATGAAGTAACGATTGCGGCACGTTTAATTGAAGCAAAAATACC
GTCCATTGAAACACTCAATATGCCGGAAGTACTGTATCGACTCGCAGAAAAACCGCAGGGACTTATTTTAGTGACAGGAC
CAACAGGTTCAGGGAAATCCACGACCTTAGCGGCAATGATTGATTACATAAATGAAACGCGTTCCAAGCATATTATTACG
CTAGAGGATCCGATTGAGTATTTGCATTCGCATAAAAAATCAGTCATCAATCAGCGTGAGGTCGGTGTAGATACGATGAG
CTTTGCCAATGGCTTACGTGCTTCGTTACGTCAGGACCCGGATATTATTTTAGTCGGTGAGATGCGCGACTATGAAACGA
TTTCAACAGCTATTACCGCAGCTGAGACAGGGCACTTGGTAATGGCAACGTTACATACTAGTAGTGCGCCAACAACAATA
GACCGTATTATTGACGTGTTTCCGCCACATCAACAAAGTCAAATCCGTGTGCAACTTGCGAATGTACTGCAAGGGATCAT
TTCACAGCGTCTCTTTGTACGCAAAGATGTAAAGGGGCGTATAGCGGCAACTGAAATTTTAGTACAGGCTCCGTCCATTT
CAAACTTAATCCGCAATGAGAAAATTCACCAAATTCCAAGTGTGATGCAGACAAGTAGGGCACTGGGCATGCACACATTG
GAATCATCCATGCAGCACTTAATCGCTACAGGGAAAATTTCATTAGAAGATGCCAGACCATATATGAATGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.898

95.977

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.376

93.966

0.483
  pilT Acinetobacter baumannii strain A118

50.299

95.977

0.483
  pilT Acinetobacter baumannii D1279779

50.299

95.977

0.483
  pilT Neisseria meningitidis 8013

50

95.977

0.48
  pilT Acinetobacter nosocomialis M2

50

95.977

0.48
  pilT Vibrio cholerae strain A1552

49.701

95.977

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.701

95.977

0.477
  pilT Neisseria gonorrhoeae MS11

49.701

95.977

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.701

95.977

0.477
  pilT Acinetobacter baylyi ADP1

49.102

95.977

0.471
  pilT Legionella pneumophila strain Lp02

49.544

94.54

0.468
  pilT Legionella pneumophila strain ERS1305867

49.544

94.54

0.468
  pilU Pseudomonas stutzeri DSM 10701

43.202

95.115

0.411
  pilU Vibrio cholerae strain A1552

43.478

92.529

0.402
  pilU Acinetobacter baylyi ADP1

39.645

97.126

0.385

Multiple sequence alignment