Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FNL97_RS04425 Genome accession   NZ_CP041632
Coordinates   875736..876794 (+) Length   352 a.a.
NCBI ID   WP_143415116.1    Uniprot ID   -
Organism   Geobacillus sp. E263     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 870736..881794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNL97_RS04410 - 871888..872670 (+) 783 WP_143415113.1 PRC-barrel domain-containing protein -
  FNL97_RS04415 - 872692..874050 (+) 1359 WP_143415114.1 VanW family protein -
  FNL97_RS04420 - 874054..875724 (+) 1671 WP_143415115.1 ATPase, T2SS/T4P/T4SS family -
  FNL97_RS04425 pilT 875736..876794 (+) 1059 WP_143415116.1 type IV pilus twitching motility protein PilT Machinery gene
  FNL97_RS04430 - 876781..877992 (+) 1212 WP_143415117.1 type II secretion system F family protein -
  FNL97_RS04435 - 878325..879998 (-) 1674 WP_015864666.1 IS200/IS605 family accessory protein TnpB-related protein -
  FNL97_RS04440 - 880262..880729 (+) 468 WP_143415118.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FNL97_RS04445 - 880740..881486 (+) 747 WP_143416289.1 A24 family peptidase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39051.36 Da        Isoelectric Point: 8.9770

>NTDB_id=372615 FNL97_RS04425 WP_143415116.1 875736..876794(+) (pilT) [Geobacillus sp. E263]
MKQRLDALLCAAFEVKASDVHLTVGVPPIFRINGELKKYGQDVLRPEDTEGMAKAVIPYHLWLRFQEKGELDFSYGIPGV
SRFRINTYKQRSCVSLAIRIIPTKIPTIDELRLPEVLKQIAQKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMRKHIITL
EDPIEYLHKHGSCIIDQREVGFDTNNFANGLRAALRQDPDVILVGEMRDLETIHTAITAAETGHLVLGTLHTSSAPATID
RIIDVFPPAQQSQIRIQLASVLVAIISQRLFPTPQKTRRVAATEVLINNAAVANLIRNEKVHQIINVMQTSRSLGMHTLA
MSIKELMQAGLILRDAAEPYLAQERGDSYASV

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=372615 FNL97_RS04425 WP_143415116.1 875736..876794(+) (pilT) [Geobacillus sp. E263]
ATGAAGCAAAGATTGGATGCTCTTTTATGTGCTGCGTTTGAAGTTAAAGCTTCCGACGTTCATTTGACGGTCGGCGTGCC
GCCGATTTTTCGCATCAATGGCGAATTAAAAAAATACGGCCAAGACGTTTTGCGGCCGGAAGATACGGAAGGAATGGCAA
AAGCGGTCATTCCGTATCATTTATGGCTGCGATTTCAGGAAAAAGGAGAATTAGATTTTTCCTACGGGATTCCCGGCGTT
TCCCGCTTCCGCATCAATACGTATAAACAGCGGTCATGTGTGTCGCTGGCGATCCGCATCATTCCGACAAAAATTCCAAC
GATTGATGAACTGCGCCTTCCTGAAGTATTAAAACAAATTGCGCAAAAACCGCACGGGCTTGTGCTAGTGACAGGCCCGA
CGGGAAGCGGGAAGTCGACGACGCTTGCCGCGATGATTGACTATATGAACAAAACGATGCGGAAACATATTATTACGCTG
GAGGATCCTATCGAATATTTGCACAAGCACGGCAGCTGCATTATTGACCAGCGCGAAGTCGGCTTTGATACGAACAATTT
TGCCAACGGTTTGCGCGCGGCGCTTCGTCAAGATCCCGATGTTATTTTAGTCGGGGAAATGCGCGATTTGGAAACGATTC
ATACCGCCATTACGGCGGCGGAAACGGGGCATCTTGTTCTTGGCACATTACATACATCGAGCGCGCCGGCGACGATCGAT
CGGATTATTGACGTGTTTCCGCCTGCCCAACAATCGCAAATTCGCATTCAGCTCGCGTCCGTTCTTGTCGCGATTATTTC
TCAGCGTCTTTTTCCAACACCGCAAAAAACAAGACGGGTCGCTGCTACGGAAGTTTTAATTAATAATGCGGCAGTCGCCA
ATTTAATTCGCAACGAAAAAGTCCATCAAATCATTAACGTTATGCAAACAAGCCGCTCCCTTGGCATGCATACGCTGGCG
ATGAGTATTAAAGAGTTAATGCAAGCCGGGCTGATCCTTCGCGATGCGGCTGAACCTTATCTTGCACAGGAGCGTGGCGA
TTCGTATGCCTCAGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.829

93.182

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

93.182

0.466
  pilT Vibrio cholerae strain A1552

50

93.182

0.466
  pilT Pseudomonas stutzeri DSM 10701

48.802

94.886

0.463
  pilT Pseudomonas aeruginosa PAK

49.39

93.182

0.46
  pilT Neisseria meningitidis 8013

46.377

98.011

0.455
  pilT Acinetobacter nosocomialis M2

48.485

93.75

0.455
  pilT Acinetobacter baumannii D1279779

49.226

91.761

0.452
  pilT Acinetobacter baumannii strain A118

49.226

91.761

0.452
  pilT Neisseria gonorrhoeae MS11

47.576

93.75

0.446
  pilT Acinetobacter baylyi ADP1

47.692

92.33

0.44
  pilT Legionella pneumophila strain ERS1305867

45.455

93.75

0.426
  pilT Legionella pneumophila strain Lp02

45.455

93.75

0.426
  pilU Pseudomonas stutzeri DSM 10701

43.544

94.602

0.412
  pilU Vibrio cholerae strain A1552

39.335

100

0.403
  pilU Acinetobacter baylyi ADP1

40.867

91.761

0.375

Multiple sequence alignment