Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G5V60_RS04450 Genome accession   NZ_CP049101
Coordinates   916730..917710 (+) Length   326 a.a.
NCBI ID   WP_001527643.1    Uniprot ID   -
Organism   Escherichia coli strain EC28     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 911730..922710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5V60_RS04420 (G5V60_04415) yggM 912331..913338 (+) 1008 WP_001527648.1 DUF1202 family protein -
  G5V60_RS04425 (G5V60_04420) hemW 913407..914543 (-) 1137 WP_001527646.1 radical SAM family heme chaperone HemW -
  G5V60_RS04430 (G5V60_04425) rdgB 914536..915129 (-) 594 WP_164699333.1 XTP/dITP diphosphatase -
  G5V60_RS04435 (G5V60_04430) yggU 915137..915427 (-) 291 WP_001277214.1 DUF167 family protein YggU -
  G5V60_RS04440 (G5V60_04435) yggT 915424..915990 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  G5V60_RS04445 (G5V60_04440) yggS 916008..916712 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  G5V60_RS04450 (G5V60_04445) pilT 916730..917710 (+) 981 WP_001527643.1 type IV pilus twitching motility protein PilT Machinery gene
  G5V60_RS04455 (G5V60_04450) ruvX 917884..918300 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  G5V60_RS04460 (G5V60_04455) yqgE 918300..918863 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  G5V60_RS04465 (G5V60_04460) gshB 918972..919922 (-) 951 WP_000593260.1 glutathione synthase -
  G5V60_RS04470 (G5V60_04465) rsmE 919935..920666 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G5V60_RS04475 (G5V60_04470) endA 920746..921453 (-) 708 WP_001305312.1 deoxyribonuclease I -
  G5V60_RS04480 (G5V60_04475) yggI 921548..922045 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36002.17 Da        Isoelectric Point: 5.7995

>NTDB_id=372390 G5V60_RS04450 WP_001527643.1 916730..917710(+) (pilT) [Escherichia coli strain EC28]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFEAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQSVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=372390 G5V60_RS04450 WP_001527643.1 916730..917710(+) (pilT) [Escherichia coli strain EC28]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGAGGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGTCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGAA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.767

97.546

0.417
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment