Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THERJR_RS08825 Genome accession   NC_014152
Coordinates   1804591..1805679 (-) Length   362 a.a.
NCBI ID   WP_282432156.1    Uniprot ID   -
Organism   Thermincola potens JR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1799591..1810679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THERJR_RS08790 (TherJR_1746) - 1800217..1800675 (-) 459 WP_041587758.1 hypothetical protein -
  THERJR_RS08795 (TherJR_1747) - 1800692..1801180 (-) 489 WP_013120609.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THERJR_RS08800 (TherJR_1748) - 1801173..1801628 (-) 456 WP_013120610.1 type II secretion system protein -
  THERJR_RS15485 (TherJR_1749) - 1801651..1802091 (-) 441 WP_013120611.1 GspH/FimT family pseudopilin -
  THERJR_RS08810 (TherJR_1750) - 1802110..1802862 (-) 753 WP_013120612.1 A24 family peptidase -
  THERJR_RS17160 (TherJR_1751) - 1802928..1803281 (-) 354 WP_013120613.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THERJR_RS08820 (TherJR_1752) - 1803315..1804529 (-) 1215 WP_013120614.1 type II secretion system F family protein -
  THERJR_RS08825 (TherJR_1753) pilT 1804591..1805679 (-) 1089 WP_282432156.1 type IV pilus twitching motility protein PilT Machinery gene
  THERJR_RS08830 (TherJR_1754) gspE 1805704..1807407 (-) 1704 WP_013120616.1 type II secretion system ATPase GspE -
  THERJR_RS15490 (TherJR_1755) - 1807576..1808052 (-) 477 WP_013120617.1 MerR family transcriptional regulator -
  THERJR_RS08840 (TherJR_1756) aroE 1808167..1809048 (-) 882 WP_013120618.1 shikimate dehydrogenase -
  THERJR_RS08845 (TherJR_1757) - 1809162..1809362 (-) 201 WP_013120619.1 hypothetical protein -
  THERJR_RS08850 (TherJR_1758) - 1809425..1809943 (-) 519 WP_013120620.1 YqeG family HAD IIIA-type phosphatase -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 39912.17 Da        Isoelectric Point: 6.5674

>NTDB_id=37233 THERJR_RS08825 WP_282432156.1 1804591..1805679(-) (pilT) [Thermincola potens JR]
MPKFSDEVLALHIEELLKMAVERGASDLHLTVNAAPVVRVHGELRHLPLPVLTYQDLKRLAAEILNEEQKVQFEEKGELD
FSYSIYGYARFRVNVFKQRGTPAIAARIIPPVILNTDELGLPEVVKTLVRKPNGLILVTGPTGSGKSTTLAAMIDQLNKE
TTGHIITLEDPIEFLHTHQNCIINQREIGLDSKTFAAGLRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHT
AGAVQTIDRIIDVFPPHQQEQIRVQLALILQGVISQTLIPRADHAGRVPAVEVLVATPAVRNMIREGKTHQLMTAMQAGA
KYGMQTMDQSLKSLYKKGLITYEEAHSRALDQDSFLTFINAV

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=37233 THERJR_RS08825 WP_282432156.1 1804591..1805679(-) (pilT) [Thermincola potens JR]
ATGCCAAAATTTTCAGACGAGGTGTTAGCGTTGCACATTGAAGAATTGTTAAAAATGGCTGTGGAACGGGGGGCTTCGGA
TCTGCACCTGACGGTGAATGCGGCGCCGGTTGTTCGGGTGCACGGTGAACTGCGCCACCTGCCGTTACCGGTTTTGACAT
ATCAGGACCTGAAACGATTGGCCGCAGAGATTTTAAACGAAGAACAAAAGGTACAGTTTGAGGAAAAAGGTGAACTGGAT
TTTTCATACAGCATTTACGGTTATGCCCGCTTCAGGGTGAATGTGTTCAAACAGCGGGGGACGCCGGCCATTGCGGCCCG
GATAATCCCGCCGGTCATTCTGAATACCGACGAGCTGGGCCTGCCCGAAGTGGTCAAAACCCTTGTCCGCAAGCCCAATG
GCCTGATTCTTGTGACAGGGCCCACCGGAAGCGGTAAATCCACCACTCTGGCGGCCATGATCGACCAGTTAAACAAAGAG
ACAACGGGGCATATCATTACCCTGGAAGACCCTATCGAATTCCTGCATACGCACCAGAACTGCATCATCAACCAGCGTGA
AATAGGTTTGGATTCCAAGACCTTTGCCGCCGGCTTGAGGGCGGCCCTCCGGCAGGACCCTGACGTGATCCTGGTCGGTG
AGATGCGCGACCTGGAGACCATTGCCACGGCCATAACGGCGGCGGAAACGGGGCACCTGGTGCTGGCCACCCTGCATACG
GCCGGCGCTGTACAGACCATTGACCGGATCATTGACGTTTTTCCGCCGCACCAGCAGGAGCAGATCAGGGTGCAGTTGGC
GCTAATCCTGCAGGGAGTGATTTCCCAGACCCTGATTCCGCGGGCTGACCATGCGGGGCGGGTGCCTGCCGTGGAAGTCC
TGGTGGCCACGCCGGCAGTGCGCAACATGATCCGGGAAGGCAAGACCCACCAGTTGATGACGGCTATGCAGGCCGGGGCC
AAGTACGGTATGCAGACTATGGACCAGTCCCTGAAGTCTCTCTATAAAAAAGGCTTGATTACTTACGAAGAAGCCCATTC
CAGGGCCTTAGACCAGGACAGTTTCCTGACTTTCATTAATGCTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.422

96.961

0.508
  pilT Acinetobacter nosocomialis M2

51.156

95.58

0.489
  pilT Acinetobacter baumannii D1279779

51.156

95.58

0.489
  pilT Acinetobacter baumannii strain A118

51.156

95.58

0.489
  pilT Pseudomonas aeruginosa PAK

51.304

95.304

0.489
  pilT Acinetobacter baylyi ADP1

51.014

95.304

0.486
  pilT Vibrio cholerae strain A1552

50.144

95.856

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.144

95.856

0.481
  pilT Pseudomonas stutzeri DSM 10701

49.565

95.304

0.472
  pilT Neisseria meningitidis 8013

48.696

95.304

0.464
  pilT Legionella pneumophila strain Lp02

48.696

95.304

0.464
  pilT Legionella pneumophila strain ERS1305867

48.696

95.304

0.464
  pilT Neisseria gonorrhoeae MS11

48.406

95.304

0.461
  pilU Pseudomonas stutzeri DSM 10701

44.179

92.541

0.409
  pilU Acinetobacter baylyi ADP1

41.667

92.818

0.387
  pilU Vibrio cholerae strain A1552

43.21

89.503

0.387

Multiple sequence alignment