Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CFLA_RS09225 Genome accession   NC_014151
Coordinates   2034142..2035278 (+) Length   378 a.a.
NCBI ID   WP_013117054.1    Uniprot ID   D5UER0
Organism   Cellulomonas flavigena DSM 20109     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2029142..2040278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFLA_RS09205 (Cfla_1819) ruvX 2029257..2029823 (+) 567 WP_245530324.1 Holliday junction resolvase RuvX -
  CFLA_RS09210 (Cfla_1820) mltG 2029831..2031036 (+) 1206 WP_013117051.1 endolytic transglycosylase MltG -
  CFLA_RS09215 (Cfla_1821) - 2031036..2031881 (+) 846 WP_013117052.1 shikimate dehydrogenase -
  CFLA_RS09220 (Cfla_1822) - 2031987..2033660 (+) 1674 WP_013117053.1 ATPase, T2SS/T4P/T4SS family -
  CFLA_RS09225 (Cfla_1823) pilT 2034142..2035278 (+) 1137 WP_013117054.1 type IV pilus twitching motility protein PilT Machinery gene
  CFLA_RS09230 (Cfla_1824) - 2035278..2036507 (+) 1230 WP_013117055.1 type II secretion system F family protein -
  CFLA_RS21185 (Cfla_1825) - 2036679..2037134 (+) 456 WP_013117056.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CFLA_RS21095 (Cfla_1826) - 2037231..2039009 (+) 1779 WP_013117057.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CFLA_RS09245 (Cfla_1827) - 2039002..2039664 (+) 663 WP_013117058.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41266.00 Da        Isoelectric Point: 6.8707

>NTDB_id=37216 CFLA_RS09225 WP_013117054.1 2034142..2035278(+) (pilT) [Cellulomonas flavigena DSM 20109]
MPIDEVLREMVRLGASDAHFTTGSPPMVRLSGALQPLEQFGPVMPDGLRRSLYAILTQKQRERYEEELELDVSYAVRGLA
RFRVNVYQQRESIGAAFRVIPYEIKPLEELGVPAVVGTFAGLPRGLVLVTGPTGSGKSTTLASIIDLANRTRQDHIMTVE
DPIEFLHRHKKSLVNQREVGADTHSFANALKHVLRQDPDIILVGEMRDLETISVALTAAETGHLVFATLHTQDAAQTIDR
VIDVFPSHQQAQVRTQLAGAIQAVVCQTLCKRADGPGRAVATEVLVATPAIRNLIREGKTHQIYSSMQAGAKQGMHTMDQ
HLADLVKQGKITYEVGLEKSHHAEDYNRLTGRFSGASQGAAGMGDEVTMGSGSYGQAF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=37216 CFLA_RS09225 WP_013117054.1 2034142..2035278(+) (pilT) [Cellulomonas flavigena DSM 20109]
ATGCCGATCGACGAGGTCCTGCGGGAGATGGTGCGCCTCGGGGCGTCCGACGCGCACTTCACGACGGGCTCGCCGCCGAT
GGTGCGGCTCTCGGGCGCGCTGCAGCCCCTCGAGCAGTTCGGGCCGGTGATGCCCGACGGCCTGCGGCGCTCGCTCTACG
CGATCCTCACGCAGAAGCAGCGGGAGCGGTACGAGGAGGAGCTCGAGCTCGACGTCTCCTACGCGGTGCGGGGTCTGGCC
CGGTTCCGCGTCAACGTCTACCAGCAGCGGGAGTCGATCGGCGCGGCGTTCCGCGTGATCCCGTACGAGATCAAGCCGCT
CGAGGAGCTCGGGGTGCCGGCCGTCGTGGGCACCTTCGCGGGGCTCCCGCGCGGGCTCGTCCTGGTGACGGGTCCGACCG
GGTCGGGCAAGTCGACGACGCTCGCCTCGATCATCGACCTCGCGAACCGCACGCGTCAGGACCACATCATGACGGTGGAG
GACCCGATCGAGTTCCTCCACCGCCACAAGAAGTCGCTGGTCAACCAGCGCGAGGTCGGTGCGGACACCCACTCGTTCGC
GAACGCGCTCAAGCACGTCCTGCGCCAGGACCCCGACATCATCCTGGTCGGGGAGATGCGTGACCTGGAGACCATCAGCG
TCGCGCTCACGGCGGCCGAGACGGGTCACCTGGTCTTCGCCACCCTGCACACGCAGGACGCCGCCCAGACGATCGACCGC
GTCATCGACGTCTTCCCGTCGCACCAGCAGGCCCAGGTCCGTACGCAGCTGGCCGGCGCGATCCAGGCGGTCGTCTGCCA
GACGCTGTGCAAGCGCGCGGACGGGCCGGGACGCGCCGTGGCGACCGAGGTGCTGGTGGCGACCCCCGCCATCCGCAACC
TCATCCGCGAGGGCAAGACGCACCAGATCTACTCCTCCATGCAGGCCGGCGCGAAGCAGGGGATGCACACGATGGACCAG
CACCTGGCGGACCTGGTGAAGCAGGGCAAGATCACCTACGAGGTGGGGCTCGAGAAGTCCCATCACGCCGAGGACTACAA
CCGGTTGACCGGGAGGTTCTCGGGGGCGTCGCAGGGCGCGGCCGGCATGGGTGACGAGGTCACGATGGGCTCCGGCTCGT
ACGGGCAGGCGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D5UER0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.657

95.767

0.495
  pilT Acinetobacter baylyi ADP1

55.856

88.095

0.492
  pilT Pseudomonas stutzeri DSM 10701

52.738

91.799

0.484
  pilT Pseudomonas aeruginosa PAK

52.89

91.534

0.484
  pilT Acinetobacter nosocomialis M2

52.601

91.534

0.481
  pilT Acinetobacter baumannii D1279779

52.601

91.534

0.481
  pilT Acinetobacter baumannii strain A118

52.601

91.534

0.481
  pilT Legionella pneumophila strain ERS1305867

52.616

91.005

0.479
  pilT Legionella pneumophila strain Lp02

52.616

91.005

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.662

87.566

0.452
  pilT Vibrio cholerae strain A1552

51.662

87.566

0.452
  pilT Neisseria meningitidis 8013

48.837

91.005

0.444
  pilT Neisseria gonorrhoeae MS11

48.547

91.005

0.442
  pilU Pseudomonas stutzeri DSM 10701

39.791

100

0.402
  pilU Vibrio cholerae strain A1552

39.118

96.032

0.376
  pilU Acinetobacter baylyi ADP1

39.429

92.593

0.365

Multiple sequence alignment