Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LPA_RS10545 Genome accession   NC_014125
Coordinates   2356687..2357721 (-) Length   344 a.a.
NCBI ID   WP_013101641.1    Uniprot ID   A0A378K9M8
Organism   Legionella pneumophila 2300/99 Alcoy     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2351687..2362721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPA_RS10525 (lpa_02928) rpoZ 2353592..2353795 (-) 204 WP_011214258.1 DNA-directed RNA polymerase subunit omega -
  LPA_RS10530 (lpa_02929) gmk 2353846..2354475 (-) 630 WP_013101639.1 guanylate kinase -
  LPA_RS10535 (lpa_02930) - 2354481..2355347 (-) 867 WP_013101640.1 YicC/YloC family endoribonuclease -
  LPA_RS10540 (lpa_02931) rph 2355648..2356355 (+) 708 WP_011946916.1 ribonuclease PH -
  LPA_RS10545 (lpa_02934) pilT 2356687..2357721 (-) 1035 WP_013101641.1 type IV pilus twitching motility protein PilT Machinery gene
  LPA_RS10550 (lpa_02936) - 2357885..2358580 (+) 696 WP_013101642.1 YggS family pyridoxal phosphate-dependent enzyme -
  LPA_RS10555 (lpa_02939) proC 2358577..2359365 (+) 789 WP_013101643.1 pyrroline-5-carboxylate reductase -
  LPA_RS10560 (lpa_02940) - 2359710..2360285 (+) 576 WP_011214264.1 YggT family protein -
  LPA_RS10565 (lpa_02941) - 2360345..2360947 (+) 603 WP_011946920.1 DUF2845 domain-containing protein -
  LPA_RS10570 (lpa_02943) - 2360961..2361242 (+) 282 WP_010947734.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38384.31 Da        Isoelectric Point: 7.0290

>NTDB_id=37149 LPA_RS10545 WP_013101641.1 2356687..2357721(-) (pilT) [Legionella pneumophila 2300/99 Alcoy]
MDIAELLAFSVKNKSSDLHISAGMPPMIRVDGDLRKINLPPLEHKDVIKIIYDIMNDRQRKEYEEFLETDFSFEIANLAR
FRVNAFNQSRGAAAVFRTIPSEILSMEDLGLPPIFKEMASFSRGLVLVTGPTGSGKSTTLAAVIDYINSTRYEHILTVED
PIEFVHQSKKCMVNQREVHKDTLSFNAALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKSMVRSMLSESLQAVVAQSLLKKNKGGRVAALEIMMCTGAIRNLIREDKIAQMYSSIQTGQAKGMQTLDQHL
TELVNKNIISKHTAHEAALNKSLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=37149 LPA_RS10545 WP_013101641.1 2356687..2357721(-) (pilT) [Legionella pneumophila 2300/99 Alcoy]
ATGGATATCGCGGAATTGTTGGCATTTTCAGTTAAAAACAAGTCATCAGATTTGCATATTTCAGCTGGCATGCCTCCTAT
GATTCGTGTTGATGGTGATTTACGCAAAATTAATTTACCTCCCTTGGAGCATAAAGACGTCATTAAGATTATTTATGACA
TAATGAATGACAGACAAAGAAAAGAATATGAAGAATTTCTTGAAACAGATTTTTCCTTTGAAATAGCCAATCTGGCGCGG
TTCAGGGTTAATGCTTTCAATCAATCACGCGGCGCCGCTGCTGTTTTCAGAACTATTCCTTCTGAAATTCTTAGCATGGA
AGATTTAGGGTTGCCCCCAATTTTTAAAGAAATGGCTAGTTTTTCCAGGGGTCTGGTTCTGGTTACTGGCCCAACTGGAT
CCGGAAAAAGTACCACGCTTGCCGCAGTCATCGATTATATTAACTCAACCCGTTATGAGCATATTTTGACAGTTGAAGAT
CCCATTGAATTTGTTCACCAAAGCAAAAAATGTATGGTCAATCAACGCGAAGTACATAAAGACACGTTGAGCTTTAATGC
TGCTTTGAGATCCGCGTTACGTGAAGATCCGGATATTATATTGGTGGGTGAATTGCGTGATTTGGAAACTATACGCCTGG
CAATGACTGCAGCAGAAACTGGGCATTTGGTTTTTGGAACATTGCATACCAATTCCGCAACAAAAACAATAAACAGGATA
ATTGATGTATTTCCAGCTGAAGAAAAATCGATGGTACGCTCTATGCTTTCTGAGTCATTACAAGCTGTCGTTGCTCAAAG
TTTATTAAAAAAGAATAAAGGTGGTCGGGTTGCGGCGCTTGAAATTATGATGTGTACCGGGGCTATTCGTAATTTAATTC
GTGAGGATAAGATAGCGCAAATGTATTCGTCAATTCAAACTGGGCAAGCCAAAGGAATGCAAACACTGGATCAACATTTG
ACTGAATTGGTTAATAAAAATATTATTTCGAAACACACCGCTCATGAGGCTGCTCTGAACAAGTCATTATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378K9M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

99.128

100

0.991
  pilT Legionella pneumophila strain Lp02

99.128

100

0.991
  pilT Acinetobacter nosocomialis M2

75.581

100

0.756
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Pseudomonas aeruginosa PAK

74.852

98.256

0.735
  pilT Acinetobacter baylyi ADP1

73.256

100

0.733
  pilT Pseudomonas stutzeri DSM 10701

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

65.103

99.128

0.645
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.477

95.64

0.645
  pilT Vibrio cholerae strain A1552

67.477

95.64

0.645
  pilT Neisseria gonorrhoeae MS11

64.809

99.128

0.642
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.497
  pilU Vibrio cholerae strain A1552

42.773

98.547

0.422
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

40.361

96.512

0.39

Multiple sequence alignment