Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FJR03_RS07265 Genome accession   NZ_CP041165
Coordinates   1421205..1422308 (+) Length   367 a.a.
NCBI ID   WP_193112866.1    Uniprot ID   A0A7M1AVV2
Organism   Sulfurimonas marina strain B2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1416205..1427308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJR03_RS07235 (FJR03_07210) - 1416897..1418027 (-) 1131 WP_193112860.1 MFS transporter -
  FJR03_RS07240 (FJR03_07215) - 1418024..1418851 (-) 828 WP_193112861.1 tyrosine-type recombinase/integrase -
  FJR03_RS07245 (FJR03_07220) - 1418852..1419847 (-) 996 WP_193112862.1 hypothetical protein -
  FJR03_RS07250 (FJR03_07225) - 1419849..1420445 (-) 597 WP_193112863.1 hypothetical protein -
  FJR03_RS07255 (FJR03_07230) gatC 1420574..1420864 (+) 291 WP_193112864.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  FJR03_RS07260 (FJR03_07235) - 1420867..1421208 (+) 342 WP_193112865.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  FJR03_RS07265 (FJR03_07240) pilT 1421205..1422308 (+) 1104 WP_193112866.1 type IV pilus twitching motility protein PilT Machinery gene
  FJR03_RS07270 (FJR03_07245) - 1422413..1423636 (+) 1224 WP_193112867.1 HD domain-containing phosphohydrolase -
  FJR03_RS07275 (FJR03_07250) cysQ 1423650..1424405 (-) 756 WP_193112868.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  FJR03_RS07280 (FJR03_07255) - 1424398..1425390 (-) 993 WP_193112869.1 SAM-dependent methyltransferase -
  FJR03_RS07285 (FJR03_07260) - 1425391..1426620 (-) 1230 WP_193112870.1 TolC family protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40856.83 Da        Isoelectric Point: 5.7166

>NTDB_id=370047 FJR03_RS07265 WP_193112866.1 1421205..1422308(+) (pilT) [Sulfurimonas marina strain B2]
MSEELQSQSAQAGKKLDIKKLLKSVMAFKSSDLHLVVGSEPQIRIDKELRALNLPVLSDTDIEEMAYSLIEDKQKKDFEE
NNELDFSFELKDIGRFRANYYRTIYGIGCAFRMIPIEIPTLEEYGNPPIFKELVKKEKGLILVTGPTGSGKSTTLASMLH
EINLNERRHIITIEDPVEFVHTNIKSLFSQRDVGSCTRSFATALKYALRQDPDVILIGEMRDAETIGAALTAAETGHLVF
GTLHTNSAPGTINRIIDVFDGEEQAQVRAQLASSLVAVISQTLIPRVGGGKVATQEVMISNPAISNLIREDKVHQIYSQM
QLNQGETAMTTQTQQLLELLQKKTISKENALKNSNRPDELMKVIANL

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=370047 FJR03_RS07265 WP_193112866.1 1421205..1422308(+) (pilT) [Sulfurimonas marina strain B2]
ATGAGTGAAGAGTTACAAAGTCAAAGTGCACAAGCAGGTAAAAAGTTAGATATTAAAAAACTTTTAAAAAGTGTTATGGC
GTTTAAGTCATCAGACTTACACCTAGTTGTTGGAAGTGAACCGCAGATTAGGATAGATAAGGAGTTAAGAGCACTTAACC
TTCCTGTTCTAAGTGATACTGACATTGAAGAGATGGCATATTCTCTTATAGAAGACAAACAGAAAAAAGATTTTGAAGAG
AACAACGAACTTGACTTCTCTTTTGAACTTAAAGATATCGGGCGTTTTCGTGCGAACTACTACCGTACTATTTACGGTAT
AGGTTGTGCATTCCGTATGATTCCTATTGAGATTCCGACACTTGAAGAGTATGGAAATCCACCAATTTTCAAAGAACTCG
TTAAAAAAGAGAAAGGGCTTATTCTTGTAACAGGTCCTACTGGTTCAGGTAAATCAACTACACTTGCGTCAATGCTACAT
GAGATTAATCTCAATGAAAGACGTCACATCATTACAATTGAAGACCCGGTTGAGTTTGTTCACACAAACATTAAATCTCT
CTTTTCACAAAGGGATGTTGGTAGCTGTACAAGATCGTTTGCTACAGCACTGAAGTACGCTCTTCGTCAAGACCCGGATG
TTATCCTCATCGGGGAGATGAGGGATGCTGAAACGATCGGTGCAGCTCTAACAGCCGCGGAAACTGGTCACTTGGTTTTT
GGTACACTCCATACAAACTCTGCACCAGGAACAATCAACCGTATCATCGACGTTTTCGATGGTGAGGAACAGGCTCAAGT
ACGTGCGCAACTTGCATCATCACTAGTTGCCGTTATTTCTCAAACACTTATTCCAAGAGTTGGTGGCGGTAAAGTTGCTA
CACAAGAGGTTATGATCTCTAATCCTGCCATTTCTAACCTTATCCGCGAAGATAAAGTACACCAAATCTATTCACAAATG
CAGTTAAACCAAGGGGAAACTGCTATGACAACTCAGACACAACAGCTGCTTGAACTCTTACAGAAAAAAACTATCTCTAA
AGAGAATGCACTGAAAAACTCAAACAGACCTGATGAGTTAATGAAGGTTATTGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(52-285)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7M1AVV2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.464

94.005

0.493
  pilT Acinetobacter baylyi ADP1

52.312

94.278

0.493
  pilT Acinetobacter nosocomialis M2

51.734

94.278

0.488
  pilT Acinetobacter baumannii D1279779

51.734

94.278

0.488
  pilT Acinetobacter baumannii strain A118

51.734

94.278

0.488
  pilT Legionella pneumophila strain Lp02

52.836

91.281

0.482
  pilT Legionella pneumophila strain ERS1305867

52.836

91.281

0.482
  pilT Pseudomonas stutzeri DSM 10701

51.304

94.005

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.952

90.736

0.471
  pilT Vibrio cholerae strain A1552

51.952

90.736

0.471
  pilT Neisseria gonorrhoeae MS11

49.133

94.278

0.463
  pilT Neisseria meningitidis 8013

48.844

94.278

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.564

95.095

0.452
  pilU Acinetobacter baylyi ADP1

40.058

94.55

0.379
  pilU Pseudomonas stutzeri DSM 10701

37.784

95.913

0.362

Multiple sequence alignment