Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FJQ87_RS15230 Genome accession   NZ_CP041151
Coordinates   3378347..3379384 (-) Length   345 a.a.
NCBI ID   WP_140933337.1    Uniprot ID   -
Organism   Shewanella sp. SNU WT4 strain SNU WT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3373347..3384384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJQ87_RS15200 (FJQ87_15200) yciH 3373984..3374313 (+) 330 WP_140934151.1 stress response translation initiation inhibitor YciH -
  FJQ87_RS15205 (FJQ87_15205) - 3374344..3374907 (+) 564 WP_140933333.1 YqgE/AlgH family protein -
  FJQ87_RS15210 (FJQ87_15210) ruvX 3374964..3375380 (+) 417 WP_140933334.1 Holliday junction resolvase RuvX -
  FJQ87_RS15215 (FJQ87_15215) hemH 3375474..3376487 (-) 1014 WP_168195206.1 ferrochelatase -
  FJQ87_RS15220 (FJQ87_15220) - 3376524..3377075 (-) 552 WP_140933335.1 glutathione peroxidase -
  FJQ87_RS15225 (FJQ87_15225) pilU 3377216..3378334 (-) 1119 WP_140933336.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FJQ87_RS15230 (FJQ87_15230) pilT 3378347..3379384 (-) 1038 WP_140933337.1 type IV pilus twitching motility protein PilT Machinery gene
  FJQ87_RS15235 (FJQ87_15235) - 3379420..3380124 (+) 705 WP_140933338.1 YggS family pyridoxal phosphate-dependent enzyme -
  FJQ87_RS15240 (FJQ87_15240) proC 3380233..3381051 (+) 819 WP_140933339.1 pyrroline-5-carboxylate reductase -
  FJQ87_RS15245 (FJQ87_15245) - 3381098..3381646 (+) 549 WP_140933340.1 YggT family protein -
  FJQ87_RS15250 (FJQ87_15250) yggU 3381651..3381950 (+) 300 WP_140933341.1 DUF167 family protein YggU -
  FJQ87_RS15255 (FJQ87_15255) - 3382048..3382488 (+) 441 WP_140933342.1 DUF4426 domain-containing protein -
  FJQ87_RS15260 (FJQ87_15260) rdgB 3382577..3383176 (+) 600 WP_140933343.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FJQ87_RS15265 (FJQ87_15265) hemW 3383176..3384315 (+) 1140 WP_140933344.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38270.83 Da        Isoelectric Point: 6.9093

>NTDB_id=369933 FJQ87_RS15230 WP_140933337.1 3378347..3379384(-) (pilT) [Shewanella sp. SNU WT4 strain SNU WT1]
MEITELLAFSVKHNASDLHLSSGVPPMIRVDGDVRKLNVPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSTILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHPNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQASF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=369933 FJQ87_RS15230 WP_140933337.1 3378347..3379384(-) (pilT) [Shewanella sp. SNU WT4 strain SNU WT1]
ATGGAAATAACCGAATTATTGGCGTTTAGTGTAAAGCATAACGCCTCAGATTTGCATTTGTCTTCTGGCGTTCCGCCAAT
GATCCGTGTTGATGGCGATGTAAGAAAGCTCAACGTGCCAGCGCTCGATCATCAAGGTGTGCATTCATTGGTTTACGACA
TTATGAATGACAAGCAGCGCAAAGACTATGAAGAGCATTTAGAAATCGACTTTTCATTTGAAGTTCCGAATCTTGCCCGT
TTCAGGGTCAATGCCTTCAATCAATCTCGTGGAGCCGCCGCTGTTTTCCGTACTATTCCGAGCACCATTTTATCGCTCGA
GCAGTTAGGCGCCCCTGAGATTTTTAAAAAGATTTCAAGCTTTCCCCGTGGCTTAGTGTTAGTCACCGGACCTACTGGTT
CAGGTAAGAGTACCACGCTCGCCGCTATGGTCGATTATATTAACGAAAATCGACATGATCATATTTTAACAATTGAAGAC
CCCATTGAATTCGTGCACCCCAACAAGCAATGCTTGATTAACCAGCGCGAAGTGCATCGTCATACTCATAGCTTTAATGC
GGCGCTGCGCTCAGCCCTGCGTGAAGACCCGGATGTTATTTTAGTGGGTGAGATGCGCGATCTTGAAACCATCCGCTTAG
CTATGACGGCCGCAGAAACCGGTCATTTAGTGTTTGGTACCTTGCATACGACCTCAGCCGCTAAAACCATTGACCGTGTG
GTTGACGTGTTTCCTGCCGGCGAGAAAGACATGGTGCGCACTATGTTGTCTGAATCCTTACAAGCCGTTATCTCGCAAAC
CTTGATTAAGAAAGTCGGTGGCGGCCGCGTGGCAGCTCATGAAATCATGATGGGCACTCCTGCCATTCGTAACTTAATCC
GTGAGGATAAAGTGGCGCAAATGTATTCAGCCATTCAAACCGGTATGTCCCATGGCATGCAAACGCTTGAGCAGTGTTTA
CAGAATTTGGTGAATCGCGGCTTAATTACTCGTGAAGATGCTATGAGTAAGAGTTCGAATAAACAGGCGAGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.717

99.42

0.783
  pilT Acinetobacter baumannii D1279779

78.717

99.42

0.783
  pilT Acinetobacter baumannii strain A118

78.717

99.42

0.783
  pilT Pseudomonas aeruginosa PAK

78.994

97.971

0.774
  pilT Pseudomonas stutzeri DSM 10701

78.07

99.13

0.774
  pilT Acinetobacter baylyi ADP1

79.635

95.362

0.759
  pilT Legionella pneumophila strain Lp02

73.9

98.841

0.73
  pilT Legionella pneumophila strain ERS1305867

73.9

98.841

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.591

97.681

0.719
  pilT Vibrio cholerae strain A1552

73.591

97.681

0.719
  pilT Neisseria meningitidis 8013

68.605

99.71

0.684
  pilT Neisseria gonorrhoeae MS11

68.314

99.71

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.399

99.42

0.412
  pilU Vibrio cholerae strain A1552

40.774

97.391

0.397
  pilU Acinetobacter baylyi ADP1

38.872

97.681

0.38

Multiple sequence alignment