Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FG174_RS21060 Genome accession   NZ_CP041132
Coordinates   4403275..4404297 (-) Length   340 a.a.
NCBI ID   WP_089197423.1    Uniprot ID   -
Organism   Serratia marcescens strain WVU-009     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4398275..4409297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FG174_RS21030 (FG174_21030) - 4399074..4399586 (+) 513 WP_033651326.1 SprT family zinc-dependent metalloprotease -
  FG174_RS21035 (FG174_21035) endA 4399688..4400383 (+) 696 WP_033644681.1 deoxyribonuclease I -
  FG174_RS21040 (FG174_21040) rsmE 4400453..4401184 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FG174_RS21045 (FG174_21045) gshB 4401195..4402145 (+) 951 WP_033651325.1 glutathione synthase -
  FG174_RS21050 (FG174_21050) - 4402293..4402856 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  FG174_RS21055 (FG174_21055) ruvX 4402856..4403278 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  FG174_RS21060 (FG174_21060) pilT 4403275..4404297 (-) 1023 WP_089197423.1 type IV pilus twitching motility protein PilT Machinery gene
  FG174_RS21065 (FG174_21065) - 4404318..4405025 (+) 708 WP_142076047.1 YggS family pyridoxal phosphate-dependent enzyme -
  FG174_RS21070 (FG174_21070) proC 4405045..4405866 (+) 822 WP_122004408.1 pyrroline-5-carboxylate reductase -
  FG174_RS21075 (FG174_21075) - 4405898..4406452 (+) 555 WP_033644671.1 YggT family protein -
  FG174_RS21080 (FG174_21080) yggU 4406449..4406739 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  FG174_RS21085 (FG174_21085) - 4406757..4407350 (+) 594 WP_142076049.1 XTP/dITP diphosphatase -
  FG174_RS21090 (FG174_21090) hemW 4407343..4408485 (+) 1143 WP_033644669.1 radical SAM family heme chaperone HemW -
  FG174_RS21095 (FG174_21095) - 4408523..4408957 (-) 435 WP_016930094.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36424.64 Da        Isoelectric Point: 7.2888

>NTDB_id=369604 FG174_RS21060 WP_089197423.1 4403275..4404297(-) (pilT) [Serratia marcescens strain WVU-009]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAQTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTATETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=369604 FG174_RS21060 WP_089197423.1 4403275..4404297(-) (pilT) [Serratia marcescens strain WVU-009]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCACAAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGACGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCAAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

53.939

97.059

0.524
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.939

97.059

0.524
  pilT Acinetobacter baylyi ADP1

50.453

97.353

0.491
  pilT Acinetobacter baumannii D1279779

50

97.059

0.485
  pilT Acinetobacter baumannii strain A118

50

97.059

0.485
  pilT Acinetobacter nosocomialis M2

50

97.059

0.485
  pilT Neisseria meningitidis 8013

49.547

97.353

0.482
  pilT Neisseria gonorrhoeae MS11

49.245

97.353

0.479
  pilT Legionella pneumophila strain ERS1305867

48.64

97.353

0.474
  pilT Legionella pneumophila strain Lp02

48.64

97.353

0.474
  pilT Pseudomonas stutzeri DSM 10701

47.774

99.118

0.474
  pilT Pseudomonas aeruginosa PAK

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

37.963

95.294

0.362

Multiple sequence alignment