Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FG171_RS20785 Genome accession   NZ_CP041129
Coordinates   4363751..4364773 (-) Length   340 a.a.
NCBI ID   WP_142002960.1    Uniprot ID   -
Organism   Serratia marcescens strain WVU-006     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4358751..4369773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FG171_RS20755 (FG171_20755) - 4359552..4360064 (+) 513 WP_033644682.1 SprT family zinc-dependent metalloprotease -
  FG171_RS20760 (FG171_20760) endA 4360166..4360861 (+) 696 WP_033644681.1 deoxyribonuclease I -
  FG171_RS20765 (FG171_20765) rsmE 4360931..4361662 (+) 732 WP_033644679.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FG171_RS20770 (FG171_20770) gshB 4361673..4362623 (+) 951 WP_033644677.1 glutathione synthase -
  FG171_RS20775 (FG171_20775) - 4362769..4363332 (+) 564 WP_004937452.1 YqgE/AlgH family protein -
  FG171_RS20780 (FG171_20780) ruvX 4363332..4363754 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  FG171_RS20785 (FG171_20785) pilT 4363751..4364773 (-) 1023 WP_142002960.1 type IV pilus twitching motility protein PilT Machinery gene
  FG171_RS20790 (FG171_20790) - 4364794..4365501 (+) 708 WP_033644673.1 YggS family pyridoxal phosphate-dependent enzyme -
  FG171_RS20795 (FG171_20795) proC 4365521..4366342 (+) 822 WP_089195158.1 pyrroline-5-carboxylate reductase -
  FG171_RS20800 (FG171_20800) - 4366374..4366928 (+) 555 WP_089195159.1 YggT family protein -
  FG171_RS20805 (FG171_20805) yggU 4366925..4367215 (+) 291 WP_074055088.1 DUF167 family protein YggU -
  FG171_RS20810 (FG171_20810) - 4367233..4367826 (+) 594 WP_042785371.1 XTP/dITP diphosphatase -
  FG171_RS20815 (FG171_20815) hemW 4367819..4368961 (+) 1143 WP_033644669.1 radical SAM family heme chaperone HemW -
  FG171_RS20820 (FG171_20820) - 4369000..4369434 (-) 435 WP_142002962.1 DUF29 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36421.68 Da        Isoelectric Point: 7.2888

>NTDB_id=369556 FG171_RS20785 WP_142002960.1 4363751..4364773(-) (pilT) [Serratia marcescens strain WVU-006]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGTTGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGVQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=369556 FG171_RS20785 WP_142002960.1 4363751..4364773(-) (pilT) [Serratia marcescens strain WVU-006]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCACCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTACGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGTGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365

Multiple sequence alignment