Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FBF90_RS21395 Genome accession   NZ_CP041124
Coordinates   4471943..4472965 (-) Length   340 a.a.
NCBI ID   WP_141998958.1    Uniprot ID   -
Organism   Serratia marcescens strain WVU-003     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4466943..4477965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBF90_RS21365 (FBF90_21365) - 4467742..4468254 (+) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -
  FBF90_RS21370 (FBF90_21370) endA 4468356..4469051 (+) 696 WP_089196263.1 deoxyribonuclease I -
  FBF90_RS21375 (FBF90_21375) rsmE 4469121..4469852 (+) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FBF90_RS21380 (FBF90_21380) gshB 4469863..4470813 (+) 951 WP_043141334.1 glutathione synthase -
  FBF90_RS21385 (FBF90_21385) - 4470961..4471524 (+) 564 WP_141998956.1 YqgE/AlgH family protein -
  FBF90_RS21390 (FBF90_21390) ruvX 4471524..4471946 (+) 423 WP_025304246.1 Holliday junction resolvase RuvX -
  FBF90_RS21395 (FBF90_21395) pilT 4471943..4472965 (-) 1023 WP_141998958.1 type IV pilus twitching motility protein PilT Machinery gene
  FBF90_RS21400 (FBF90_21400) - 4472986..4473693 (+) 708 WP_141998959.1 YggS family pyridoxal phosphate-dependent enzyme -
  FBF90_RS21405 (FBF90_21405) proC 4473713..4474534 (+) 822 WP_025304249.1 pyrroline-5-carboxylate reductase -
  FBF90_RS21410 (FBF90_21410) - 4474566..4475120 (+) 555 WP_025304250.1 YggT family protein -
  FBF90_RS21415 (FBF90_21415) yggU 4475117..4475407 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  FBF90_RS21420 (FBF90_21420) - 4475425..4476018 (+) 594 WP_043141349.1 XTP/dITP diphosphatase -
  FBF90_RS21425 (FBF90_21425) hemW 4476011..4477153 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  FBF90_RS21430 (FBF90_21430) - 4477191..4477439 (-) 249 WP_025304253.1 DUF2442 domain-containing protein -
  FBF90_RS21435 (FBF90_21435) - 4477423..4477665 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36411.60 Da        Isoelectric Point: 7.0670

>NTDB_id=369509 FBF90_RS21395 WP_141998958.1 4471943..4472965(-) (pilT) [Serratia marcescens strain WVU-003]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANIFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSASQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=369509 FBF90_RS21395 WP_141998958.1 4471943..4472965(-) (pilT) [Serratia marcescens strain WVU-003]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCACAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGGGCGGGGAG
CGGTTGCGCGCCAACATTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATTGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCAGCGCCGGCCATCGTGCCGGCGCTGCTGCGGCGCGACGATGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCAGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCACATTTTGACGCTGGAG
GATCCGATTGAATTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTTGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCTGATGTGATCCTGCTGGGCGAGCTGCGCGATACCACCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGTCGCAGGCAGTGGAACGG
TTAGTGGACGTGTTTCCGGCGGAAGAAAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGTCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGACGCGTGGCGATCTTCGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCACAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATTGACGCCGGTGTGTTGGGGGACTGTGCGGGGGAGGGAACGGGTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50

97.647

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.665

99.118

0.482
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.737

100

0.45
  pilU Vibrio cholerae strain A1552

39.571

95.882

0.379
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371

Multiple sequence alignment