Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FIV34_RS04315 Genome accession   NZ_CP041046
Coordinates   956417..957526 (-) Length   369 a.a.
NCBI ID   WP_139980025.1    Uniprot ID   A0A4Y5Z004
Organism   Luteibacter pinisoli strain MAH-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 951417..962526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV34_RS04290 (FIV34_04290) - 951570..952100 (-) 531 WP_170207513.1 hypothetical protein -
  FIV34_RS04295 (FIV34_04295) - 952526..953731 (-) 1206 WP_139980016.1 glycosyltransferase family 39 protein -
  FIV34_RS04300 (FIV34_04300) - 953794..954426 (-) 633 WP_139980018.1 HAD hydrolase-like protein -
  FIV34_RS04305 (FIV34_04305) - 954426..955607 (-) 1182 WP_139980021.1 phosphoglycerate kinase -
  FIV34_RS04310 (FIV34_04310) maiA 955688..956335 (+) 648 WP_211352709.1 maleylacetoacetate isomerase -
  FIV34_RS04315 (FIV34_04315) pilU 956417..957526 (-) 1110 WP_139980025.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIV34_RS04320 (FIV34_04320) - 957620..958195 (+) 576 WP_139980027.1 DUF4398 domain-containing protein -
  FIV34_RS04325 (FIV34_04325) - 958195..958860 (+) 666 WP_170207514.1 DUF4398 domain-containing protein -
  FIV34_RS04330 (FIV34_04330) - 959125..959355 (+) 231 WP_139980029.1 YdcH family protein -
  FIV34_RS04335 (FIV34_04335) - 959540..960910 (+) 1371 WP_139980032.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41117.35 Da        Isoelectric Point: 6.5082

>NTDB_id=369157 FIV34_RS04315 WP_139980025.1 956417..957526(-) (pilU) [Luteibacter pinisoli strain MAH-14]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEIGMVIRAIKSEIPSLEELRLPSIFRDLIMEPRGLILVVGATGSGKSTTLAAMLDHRNTNSSGHILTIED
PIEYLHRHKRSIVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTETMEAAISFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVLGKEGRRIPATEVLLNTPLIRDMIRRGQIHEVKDAMDRSLQEGMQTFDQSL
YRLYREGQITLDECLNKADSRDGLALKIRLSEGATGTEFASSDPYGIGV

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=369157 FIV34_RS04315 WP_139980025.1 956417..957526(-) (pilU) [Luteibacter pinisoli strain MAH-14]
GTGGATATCGGTTATTTCCTGAAACTGATGGTGGACAAGGGCGCGTCGGACATGTTCCTGACGACCGGTGCCCCGGTGAA
CATCAAGGTGGAAGGCAAGCTTTACCCGCTTGGCAACACGGGCCTGCCCAGCGGCATGGTCAAGAAGATCGCCTATTCCC
TGATGGACGAAGGCCAGGTGCCCCAGTTCGAGCGCGACCTTGAGCTGAACATGGCGCTGGCCGTGAAGGAAGCGGGCCGC
TTCCGTATCAACGTGTTCAAGCAGCGTGGCGAGATCGGCATGGTCATCCGTGCCATCAAGAGCGAGATTCCCTCGCTGGA
AGAGCTGCGCCTGCCGTCGATCTTCCGCGACCTGATCATGGAGCCGCGCGGCCTGATCCTGGTCGTCGGTGCCACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCCATGCTCGACCACCGCAACACCAACAGCTCGGGCCATATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAGCGCTCCATCGTCAACCAGCGTGAGGTGGGCCTTGATACCCACAGCTATCACGA
GGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCGACACCGAGACCATGGAGGCCG
CCATCTCGTTCTCGGAAACCGGCCATCTCTGCCTGGCTACCCTGCACTCCAACAACGCGGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCGGAATCGGCACACAAGAACGTGCTGATGAACCTGGCCCTGAACCTGCGCGCGGTCATCAGCCAGCG
CCTGGTGCTGGGCAAGGAAGGCCGCCGCATCCCGGCGACCGAGGTGCTGCTCAACACCCCGCTGATCCGCGACATGATCC
GCCGCGGCCAGATCCACGAGGTGAAGGACGCCATGGACCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTG
TACCGCCTGTACCGCGAAGGCCAGATCACCCTCGACGAGTGCCTGAACAAGGCGGACTCGCGCGATGGCCTGGCGCTGAA
GATCCGCCTGTCCGAAGGAGCGACGGGGACGGAGTTTGCGTCCAGCGATCCTTATGGGATTGGGGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y5Z004

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.552

98.103

0.496
  pilU Vibrio cholerae strain A1552

49.322

100

0.493
  pilU Acinetobacter baylyi ADP1

49.438

96.477

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.716

99.187

0.434
  pilT Acinetobacter baylyi ADP1

43.202

89.702

0.388
  pilT Pseudomonas stutzeri DSM 10701

41.42

91.599

0.379
  pilT Pseudomonas aeruginosa PAK

41.42

91.599

0.379
  pilT Acinetobacter baumannii strain A118

42.424

89.431

0.379
  pilT Acinetobacter baumannii D1279779

42.424

89.431

0.379
  pilT Acinetobacter nosocomialis M2

42.424

89.431

0.379
  pilT Legionella pneumophila strain Lp02

41.212

89.431

0.369
  pilT Legionella pneumophila strain ERS1305867

41.212

89.431

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.059

91.328

0.366
  pilT Vibrio cholerae strain A1552

40.059

91.328

0.366
  pilT Neisseria gonorrhoeae MS11

38.617

94.038

0.363
  pilT Neisseria meningitidis 8013

38.617

94.038

0.363

Multiple sequence alignment