Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FHN83_RS07495 Genome accession   NZ_CP040889
Coordinates   1208524..1209501 (-) Length   325 a.a.
NCBI ID   WP_139563591.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain Z96-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1203524..1214501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FHN83_RS07465 (FHN83_07465) endA 1203991..1204698 (+) 708 WP_039029805.1 deoxyribonuclease I -
  FHN83_RS07470 (FHN83_07470) rsmE 1204814..1205545 (+) 732 WP_138370490.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FHN83_RS07475 (FHN83_07475) gshB 1205565..1206512 (+) 948 WP_039029804.1 glutathione synthase -
  FHN83_RS07480 (FHN83_07480) - 1206724..1207284 (+) 561 WP_039029803.1 YqgE/AlgH family protein -
  FHN83_RS07485 (FHN83_07485) ruvX 1207284..1207700 (+) 417 WP_139563590.1 Holliday junction resolvase RuvX -
  FHN83_RS07490 (FHN83_07490) - 1207741..1208430 (-) 690 WP_039029801.1 hypothetical protein -
  FHN83_RS07495 (FHN83_07495) pilT 1208524..1209501 (-) 978 WP_139563591.1 type IV pilus twitching motility protein PilT Machinery gene
  FHN83_RS07500 (FHN83_07500) - 1209519..1210220 (+) 702 WP_138370486.1 YggS family pyridoxal phosphate-dependent enzyme -
  FHN83_RS07505 (FHN83_07505) - 1210242..1210808 (+) 567 WP_039029798.1 YggT family protein -
  FHN83_RS07510 (FHN83_07510) yggU 1210805..1211101 (+) 297 WP_039029797.1 DUF167 family protein YggU -
  FHN83_RS07515 (FHN83_07515) - 1211105..1211698 (+) 594 WP_139563592.1 XTP/dITP diphosphatase -
  FHN83_RS07520 (FHN83_07520) hemW 1211691..1212833 (+) 1143 WP_139563593.1 radical SAM family heme chaperone HemW -
  FHN83_RS07525 (FHN83_07525) - 1212897..1213235 (+) 339 WP_139563594.1 endonuclease domain-containing protein -
  FHN83_RS07530 (FHN83_07530) - 1213317..1214036 (-) 720 WP_138370482.1 DUF2884 domain-containing protein -
  FHN83_RS07535 (FHN83_07535) - 1214089..1214415 (-) 327 WP_039029792.1 YggL family protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35685.79 Da        Isoelectric Point: 6.9184

>NTDB_id=368059 FHN83_RS07495 WP_139563591.1 1208524..1209501(-) (pilT) [Leclercia adecarboxylata strain Z96-1]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMEFAPFPGPDVAMLLKSWLSDEQQGAWCAQGQVDFAVALEHHRLRAS
AFAHTQGCSLALRLLAEQCPQLRTLGTPRAIPELLTNDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCPSFADGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDAF
PAQEKEPVRKQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGRTWQLPGVIQTGQQAGMQNFDQSLAERR
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=368059 FHN83_RS07495 WP_139563591.1 1208524..1209501(-) (pilT) [Leclercia adecarboxylata strain Z96-1]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCGCCACCACG
CTGGCGGCGGCGCGGGAAAATGGAATTCGCCCCTTTTCCGGGACCGGATGTCGCCATGCTGCTAAAAAGCTGGCTCAGCG
ATGAGCAGCAGGGAGCCTGGTGCGCCCAGGGACAGGTGGATTTTGCCGTCGCGCTGGAGCACCACCGGCTGCGGGCCAGC
GCCTTTGCCCATACGCAAGGCTGTTCGCTGGCGCTGCGGCTGCTTGCAGAGCAGTGTCCACAGCTGCGAACCCTGGGCAC
GCCGCGGGCGATACCCGAGCTGCTGACCAACGATAACGGCCTGATTCTGGTGACCGGGGCCACCGGCAGCGGCAAATCCA
CCACCCTTGCGGCCATGGTCGATTTTCTCAATCACCACAGCGACGGGCATATTCTGACTCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAGCGCTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGTCCGTCGTTTGCCGATGGGTTACGGGC
GGCCCTGCGTGAAGATCCGGATGTGATCCTGCTGGGCGAACTGCGTGACAGCGAGACGATCCGCCTTGCGCTCACCGCCG
CCGAGACGGGGCATCTGGTGCTGGCGACCCTGCACACCCGGGGAGCGGCTCAGGCGGTAGAGAGGCTAGTGGATGCCTTC
CCCGCACAGGAAAAAGAGCCGGTACGTAAACAGCTGGCGGGCAGCCTGCGCGCCGTGCTGGCGCAAAAGCTCGAAAGGGA
TAACCAGCAGGGTCGCGTCGCACTGTTTGAGCTGCTGGTGAATACCCCTGCCGCCGCGAATTTGATCCGCGAAGGTAGAA
CCTGGCAACTGCCCGGCGTGATCCAGACCGGGCAGCAGGCAGGCATGCAGAATTTCGATCAGAGCCTTGCCGAGCGCCGG
GCACAGGGGCGGCTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.012

100

0.483
  pilT Vibrio cholerae strain A1552

48.012

100

0.483
  pilT Neisseria meningitidis 8013

47.256

100

0.477
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.474
  pilT Legionella pneumophila strain ERS1305867

47.205

99.077

0.468
  pilT Legionella pneumophila strain Lp02

47.205

99.077

0.468
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.465
  pilT Acinetobacter baylyi ADP1

45.566

100

0.458
  pilT Acinetobacter baumannii D1279779

45.566

100

0.458
  pilT Acinetobacter baumannii strain A118

45.566

100

0.458
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.455
  pilT Acinetobacter nosocomialis M2

45.938

98.462

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

99.077

0.425
  pilU Vibrio cholerae strain A1552

40.187

98.769

0.397
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.388
  pilU Acinetobacter baylyi ADP1

36.923

100

0.369

Multiple sequence alignment