Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MRUB_RS11175 Genome accession   NC_013946
Coordinates   2268134..2269240 (-) Length   368 a.a.
NCBI ID   WP_013014466.1    Uniprot ID   D3PKU4
Organism   Meiothermus ruber DSM 1279     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2263134..2274240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRUB_RS11155 (Mrub_2211) - 2264004..2266031 (+) 2028 WP_013014462.1 M3 family metallopeptidase -
  MRUB_RS11160 (Mrub_2212) - 2266040..2266315 (-) 276 WP_013014463.1 hypothetical protein -
  MRUB_RS11165 (Mrub_2213) - 2266403..2267533 (-) 1131 WP_013014464.1 RNA-guided endonuclease TnpB family protein -
  MRUB_RS16280 - 2267526..2267981 (-) 456 WP_156113857.1 transposase -
  MRUB_RS11175 (Mrub_2215) pilT 2268134..2269240 (-) 1107 WP_013014466.1 type IV pilus twitching motility protein PilT Machinery gene
  MRUB_RS11180 (Mrub_2216) - 2269353..2269892 (-) 540 WP_013014467.1 hypothetical protein -
  MRUB_RS11185 (Mrub_2217) - 2269952..2270659 (-) 708 WP_015586782.1 nucleotidyltransferase family protein -
  MRUB_RS11190 (Mrub_2218) - 2270723..2270932 (+) 210 WP_013014469.1 DUF3248 domain-containing protein -
  MRUB_RS11195 (Mrub_2219) - 2270933..2271364 (+) 432 WP_013014470.1 DUF3809 family protein -
  MRUB_RS11200 (Mrub_2220) - 2271426..2272103 (+) 678 WP_013014471.1 metal-dependent hydrolase -
  MRUB_RS11205 (Mrub_2221) - 2272136..2273005 (+) 870 WP_036198543.1 serine/threonine-protein kinase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40487.49 Da        Isoelectric Point: 6.9245

>NTDB_id=36686 MRUB_RS11175 WP_013014466.1 2268134..2269240(-) (pilT) [Meiothermus ruber DSM 1279]
MSKAPDIVDLLNLAVDRSASDLVITVGLPPMVKIDGEFHPTEFEPLTPQETRRLTYALMDEKQQRVFEEEKELDFSFSLP
GKGRFRVNIFLQRGSVGGVLRVVPSNVKSFEELGLPKTVADIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKRCHIV
TIEDPIEFFHRHKSSIINQREIGSDTHGFDKALRSVLRQAPDVILVGEMRDYETISAAITAAETGHLVMGTLHTNSAPET
VDRIIDVFPESQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMVATPAVRALIREGKSHQLVSVIQTGGQYGMIT
MDANLADLYKRKLITYEMGQSRAVDPKEFARLANAGGPAPQTAGVRRP

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=36686 MRUB_RS11175 WP_013014466.1 2268134..2269240(-) (pilT) [Meiothermus ruber DSM 1279]
ATGAGCAAAGCACCCGATATTGTAGACCTGCTCAATCTGGCTGTAGATCGCAGCGCCTCGGACCTGGTTATCACGGTGGG
CCTTCCCCCCATGGTAAAAATTGACGGGGAGTTTCATCCCACCGAGTTTGAACCCCTCACCCCTCAGGAAACCCGGCGCC
TGACCTATGCCCTGATGGACGAGAAGCAGCAGCGGGTTTTTGAGGAAGAAAAGGAGCTTGACTTCTCCTTTAGCCTTCCG
GGTAAAGGCCGCTTCCGCGTCAACATCTTTTTGCAGCGCGGTAGTGTGGGTGGGGTGCTGCGGGTGGTGCCCTCCAATGT
CAAGAGCTTTGAGGAGCTGGGGCTACCCAAGACCGTGGCCGATATTGCCATGAGCCCCCGCGGCCTGGTGCTGGTTACTG
GCCCTACCGGCTCGGGCAAGTCCACCACCTTGGCCTCCATGATCGACTACATCAACGAGCGTAAGCGCTGCCACATCGTA
ACCATCGAAGACCCCATCGAGTTCTTCCACCGCCACAAGTCCTCCATCATCAACCAGCGCGAGATTGGCTCCGATACCCA
CGGCTTCGACAAGGCCCTGCGCTCGGTGCTGCGGCAGGCCCCCGATGTGATCCTGGTGGGGGAGATGCGCGACTATGAGA
CCATTTCGGCTGCCATCACCGCCGCCGAGACCGGGCACCTGGTTATGGGCACGCTGCACACCAATAGCGCCCCCGAGACC
GTCGACCGCATTATTGACGTCTTCCCGGAGTCTCAACAGGAGCAGGTACGGGTGCAGCTCTCCAACAACCTGGTTGCAGT
GCTCACCCAGCAGCTTCTGCCCAAAGCCTTTGGCGGTGGACGGGTGCTGGCCTACGAGCTCATGGTGGCCACCCCGGCGG
TGCGGGCGTTGATCCGCGAAGGCAAGAGCCACCAACTGGTCAGCGTGATTCAAACTGGGGGCCAGTACGGCATGATTACC
ATGGATGCCAACCTGGCCGACCTGTACAAGCGCAAGCTGATTACCTACGAGATGGGCCAGTCGCGCGCTGTAGACCCCAA
GGAGTTTGCGCGCCTGGCCAATGCCGGTGGCCCCGCACCCCAGACGGCCGGTGTGCGCCGCCCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3PKU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

68.927

96.196

0.663
  pilT Acinetobacter baylyi ADP1

54.07

93.478

0.505
  pilT Acinetobacter nosocomialis M2

53.779

93.478

0.503
  pilT Acinetobacter baumannii D1279779

53.779

93.478

0.503
  pilT Acinetobacter baumannii strain A118

53.779

93.478

0.503
  pilT Vibrio cholerae strain A1552

52.754

93.75

0.495
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.754

93.75

0.495
  pilT Pseudomonas aeruginosa PAK

52.907

93.478

0.495
  pilT Pseudomonas stutzeri DSM 10701

52.616

93.478

0.492
  pilT Neisseria meningitidis 8013

51.304

93.75

0.481
  pilT Neisseria gonorrhoeae MS11

50.581

93.478

0.473
  pilT Legionella pneumophila strain ERS1305867

50

93.478

0.467
  pilT Legionella pneumophila strain Lp02

50

93.478

0.467
  pilU Pseudomonas stutzeri DSM 10701

46.646

89.13

0.416
  pilU Acinetobacter baylyi ADP1

42.319

93.75

0.397
  pilU Vibrio cholerae strain A1552

44.839

84.239

0.378

Multiple sequence alignment