Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DACET_RS06695 Genome accession   NC_013943
Coordinates   1377939..1379009 (+) Length   356 a.a.
NCBI ID   WP_013010628.1    Uniprot ID   -
Organism   Denitrovibrio acetiphilus DSM 12809     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1372939..1384009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DACET_RS06660 (Dacet_1327) nusB 1373058..1373501 (+) 444 WP_013010621.1 transcription antitermination factor NusB -
  DACET_RS06665 (Dacet_1328) - 1373491..1373910 (+) 420 WP_013010622.1 hypothetical protein -
  DACET_RS06670 (Dacet_1329) - 1373894..1374142 (+) 249 WP_013010623.1 DUF4911 domain-containing protein -
  DACET_RS06675 (Dacet_1330) - 1374132..1375502 (+) 1371 WP_148214157.1 TldD/PmbA family protein -
  DACET_RS06680 (Dacet_1331) - 1375504..1376412 (+) 909 WP_013010625.1 HD domain-containing protein -
  DACET_RS06685 (Dacet_1332) - 1376433..1377104 (+) 672 WP_013010626.1 DUF502 domain-containing protein -
  DACET_RS06690 (Dacet_1333) aroE 1377118..1377927 (+) 810 WP_013010627.1 shikimate dehydrogenase -
  DACET_RS06695 (Dacet_1334) pilT 1377939..1379009 (+) 1071 WP_013010628.1 type IV pilus twitching motility protein PilT Machinery gene
  DACET_RS16440 - 1379053..1379196 (+) 144 WP_211204109.1 hypothetical protein -
  DACET_RS16255 - 1379243..1379404 (-) 162 WP_169304200.1 hypothetical protein -
  DACET_RS06700 (Dacet_1335) - 1379496..1380758 (-) 1263 WP_013010629.1 TolC family protein -
  DACET_RS06705 (Dacet_1336) - 1381030..1381461 (-) 432 WP_013010630.1 MarR family transcriptional regulator -
  DACET_RS06710 (Dacet_1337) - 1381725..1382414 (+) 690 WP_013010631.1 TIGR00730 family Rossman fold protein -
  DACET_RS06715 (Dacet_1338) - 1382411..1383076 (+) 666 WP_013010632.1 histidinol phosphate phosphatase domain-containing protein -
  DACET_RS06720 (Dacet_1339) - 1383077..1383655 (+) 579 WP_013010633.1 hypothetical protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39600.62 Da        Isoelectric Point: 6.4987

>NTDB_id=36667 DACET_RS06695 WP_013010628.1 1377939..1379009(+) (pilT) [Denitrovibrio acetiphilus DSM 12809]
MYNLQELLARMVQEGASDLHITAGTPPVLRINGSLVRMDGAPMTGADTKSLCFSILNDQQKKRFEDDWELDFSFGVENLS
RFRVNVFMQRGAVAAAFRVIPYDIRAFDQLNLPPVVQELCKRPRGLVLVTGPTGSGKSTTLAAMIDKINSEDPVHILTVE
DPIEFIHQHKVATVNQRELNSDTKSFKSALKYLLRQDPDVCLIGEMRDTETIEAAMTIAETGHLCMGTLHTNSAVQTVNR
IIDVFPPYQQSQIRALLSFVLEGVISQQLVQTADGKGRVMMCEVLVPTSAIRNLIREDKLHQVYGAMQTGQGEHGMQTMS
QSLLAAFKNGLITKNEAIARAVYPEEVRQMLKREGL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=36667 DACET_RS06695 WP_013010628.1 1377939..1379009(+) (pilT) [Denitrovibrio acetiphilus DSM 12809]
ATGTATAATCTGCAGGAACTACTGGCTAGAATGGTTCAGGAAGGCGCAAGCGACCTGCATATTACAGCAGGCACACCGCC
TGTGCTGAGGATAAACGGCAGCCTTGTCCGTATGGACGGCGCTCCTATGACCGGAGCCGACACAAAGTCTTTATGTTTCA
GTATTCTGAACGACCAGCAAAAAAAACGCTTTGAAGATGACTGGGAACTTGATTTCTCTTTCGGAGTGGAGAATCTCAGC
AGATTCCGTGTGAATGTCTTTATGCAAAGGGGAGCTGTAGCCGCAGCTTTCAGAGTTATACCCTATGATATCCGTGCTTT
TGACCAGCTTAACCTGCCCCCTGTTGTGCAGGAGCTTTGTAAACGTCCGAGGGGGCTTGTCCTTGTTACCGGTCCAACCG
GAAGCGGTAAGTCAACGACCCTTGCAGCTATGATAGATAAGATTAATTCTGAAGACCCTGTACATATTCTGACTGTGGAA
GATCCTATCGAATTTATCCACCAGCATAAGGTGGCAACTGTAAACCAGCGTGAGCTTAACTCAGATACAAAATCGTTTAA
ATCAGCCCTTAAGTATTTACTGCGTCAGGATCCTGATGTATGCCTTATTGGTGAAATGCGCGACACAGAGACTATAGAGG
CCGCGATGACTATTGCAGAGACAGGGCATCTTTGTATGGGGACACTTCATACTAACAGTGCAGTACAGACTGTTAACCGT
ATAATCGATGTTTTCCCACCCTATCAGCAGTCCCAGATAAGGGCTTTACTGTCCTTTGTTCTCGAAGGTGTAATCTCTCA
ACAACTGGTACAGACAGCCGACGGAAAAGGGCGTGTCATGATGTGTGAGGTGCTTGTTCCTACATCAGCCATCCGCAACC
TAATCAGGGAAGACAAACTTCATCAGGTTTACGGTGCGATGCAGACAGGGCAGGGGGAGCATGGTATGCAGACAATGAGT
CAATCTCTGCTCGCTGCTTTTAAAAATGGTTTGATAACAAAAAATGAGGCGATCGCAAGAGCTGTTTATCCAGAAGAAGT
CAGACAGATGCTTAAACGCGAAGGCTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

53.433

94.101

0.503
  pilT Legionella pneumophila strain ERS1305867

53.433

94.101

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.287

97.753

0.492
  pilT Acinetobacter baylyi ADP1

51.02

96.348

0.492
  pilT Acinetobacter nosocomialis M2

50.437

96.348

0.486
  pilT Acinetobacter baumannii D1279779

50.437

96.348

0.486
  pilT Acinetobacter baumannii strain A118

50.437

96.348

0.486
  pilT Pseudomonas aeruginosa PAK

49.854

96.348

0.48
  pilT Neisseria meningitidis 8013

49.419

96.629

0.478
  pilT Neisseria gonorrhoeae MS11

49.419

96.629

0.478
  pilT Pseudomonas stutzeri DSM 10701

49.563

96.348

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.401

93.82

0.463
  pilT Vibrio cholerae strain A1552

49.401

93.82

0.463
  pilU Vibrio cholerae strain A1552

45.272

98.034

0.444
  pilU Pseudomonas stutzeri DSM 10701

43.553

98.034

0.427
  pilU Acinetobacter baylyi ADP1

41.026

98.596

0.404

Multiple sequence alignment