Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DEFDS_RS05575 Genome accession   NC_013939
Coordinates   1104049..1105137 (+) Length   362 a.a.
NCBI ID   WP_013007826.1    Uniprot ID   D3PDA6
Organism   Deferribacter desulfuricans SSM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1099049..1110137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DEFDS_RS05555 (DEFDS_1106) upp 1100262..1100897 (+) 636 WP_013007822.1 uracil phosphoribosyltransferase -
  DEFDS_RS05560 (DEFDS_1107) - 1100900..1101550 (+) 651 WP_013007823.1 DUF502 domain-containing protein -
  DEFDS_RS05565 (DEFDS_1108) aroE 1101537..1102340 (+) 804 WP_070206103.1 shikimate dehydrogenase -
  DEFDS_RS05570 (DEFDS_1109) pilB 1102341..1104038 (+) 1698 WP_013007825.1 type IV-A pilus assembly ATPase PilB -
  DEFDS_RS05575 (DEFDS_1110) pilT 1104049..1105137 (+) 1089 WP_013007826.1 type IV pilus twitching motility protein PilT Machinery gene
  DEFDS_RS05580 (DEFDS_1111) - 1105155..1106363 (+) 1209 WP_013007827.1 type II secretion system F family protein -
  DEFDS_RS05585 (DEFDS_1112) - 1106360..1107880 (+) 1521 WP_013007828.1 sensor histidine kinase -
  DEFDS_RS05590 (DEFDS_1113) - 1107882..1108286 (-) 405 WP_013007829.1 RrF2 family transcriptional regulator -
  DEFDS_RS05595 (DEFDS_1114) - 1108452..1108850 (+) 399 WP_013007830.1 DUF3568 family protein -
  DEFDS_RS05600 (DEFDS_1115) - 1108843..1109292 (+) 450 WP_013007831.1 N-acetyltransferase -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40959.46 Da        Isoelectric Point: 7.5064

>NTDB_id=36639 DEFDS_RS05575 WP_013007826.1 1104049..1105137(+) (pilT) [Deferribacter desulfuricans SSM1]
MYSLQDLLKKMIEINASDLHITVGTQPVYRVNGELVRIDGEVLTPSVTKQMCYSILTEAQKKRFEEEWELDFSFGIKGVS
RFRANIFMQRGAVAAAFRRIPYKVMGFEELGLPPIIKTLCDKPRGLILVTGPTGSGKSTTLAAMIDKINNEKKVHIITIE
DPIEYLHPHKQAVVNQREVHADTKSFITALKYILRQDPDVVLIGEMRDLETIEAALTVSETGHLTFGTLHTNSAVQTINR
IIDVFPPHQQPQIRAQLSFVLEGVICQQLLQRADGKGRVLACEVLIPNPAIRNLIREDKLHQIYSMMQTGQEEHGMITMS
QSLYELYKKGLITYEDAINRAVYPDEVRNMIDQGGIKNRGRF

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=36639 DEFDS_RS05575 WP_013007826.1 1104049..1105137(+) (pilT) [Deferribacter desulfuricans SSM1]
ATGTATTCATTGCAGGATTTATTGAAAAAGATGATAGAAATTAATGCAAGTGATTTACACATTACAGTTGGTACACAACC
AGTTTATAGAGTAAATGGTGAGCTTGTAAGAATTGATGGGGAAGTTTTAACACCATCCGTTACAAAACAGATGTGTTATT
CTATATTGACAGAAGCTCAAAAGAAAAGATTTGAAGAGGAGTGGGAATTAGACTTCTCTTTTGGAATTAAAGGTGTGAGT
AGGTTCAGGGCAAATATTTTTATGCAAAGAGGTGCAGTAGCTGCAGCTTTTAGAAGAATCCCTTATAAAGTTATGGGTTT
TGAAGAGTTGGGATTGCCACCAATTATTAAAACATTATGTGATAAACCGAGAGGACTCATATTAGTAACTGGGCCTACAG
GGAGCGGGAAGTCTACAACACTTGCTGCTATGATTGATAAAATTAATAATGAGAAAAAAGTGCATATTATTACAATAGAA
GACCCAATAGAATATCTTCATCCGCATAAACAGGCAGTTGTTAACCAAAGAGAAGTTCATGCCGATACTAAGTCTTTTAT
CACAGCATTGAAGTATATTTTAAGGCAAGACCCGGATGTTGTTTTAATAGGTGAGATGAGAGATTTAGAAACAATTGAAG
CAGCTTTGACTGTATCTGAAACTGGTCACCTGACTTTTGGAACATTGCATACAAATAGTGCCGTACAAACTATTAACAGA
ATCATTGATGTTTTTCCTCCTCACCAGCAGCCACAAATTAGGGCACAGCTGTCGTTTGTTTTAGAAGGGGTAATATGTCA
ACAGTTGCTACAGAGAGCAGATGGAAAAGGGAGAGTGCTTGCTTGTGAAGTTTTGATACCAAATCCAGCTATAAGAAATC
TAATTAGAGAAGATAAATTACATCAAATTTACTCAATGATGCAAACTGGTCAGGAAGAGCATGGAATGATTACAATGAGT
CAATCGCTATATGAGCTTTATAAAAAAGGTCTTATAACTTATGAAGATGCAATTAATAGAGCAGTATACCCTGATGAAGT
AAGAAATATGATTGATCAAGGTGGAATTAAAAATAGAGGTAGATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3PDA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.936

94.751

0.511
  pilT Pseudomonas aeruginosa PAK

52.339

94.475

0.494
  pilT Acinetobacter baylyi ADP1

52.047

94.475

0.492
  pilT Acinetobacter nosocomialis M2

51.754

94.475

0.489
  pilT Acinetobacter baumannii D1279779

51.754

94.475

0.489
  pilT Acinetobacter baumannii strain A118

51.754

94.475

0.489
  pilT Legionella pneumophila strain Lp02

51.163

95.028

0.486
  pilT Legionella pneumophila strain ERS1305867

51.163

95.028

0.486
  pilT Pseudomonas stutzeri DSM 10701

51.462

94.475

0.486
  pilT Neisseria meningitidis 8013

50.888

93.37

0.475
  pilT Neisseria gonorrhoeae MS11

50.888

93.37

0.475
  pilT Vibrio cholerae strain A1552

48.649

91.989

0.448
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.649

91.989

0.448
  pilU Vibrio cholerae strain A1552

43.966

96.133

0.423
  pilU Acinetobacter baylyi ADP1

43.516

95.856

0.417
  pilU Pseudomonas stutzeri DSM 10701

43.02

96.961

0.417

Multiple sequence alignment