Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DEFDS_RS03120 Genome accession   NC_013939
Coordinates   597461..598519 (-) Length   352 a.a.
NCBI ID   WP_013007354.1    Uniprot ID   -
Organism   Deferribacter desulfuricans SSM1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 592461..603519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DEFDS_RS03100 (DEFDS_0621) - 593776..594153 (-) 378 WP_013007350.1 DUF1992 domain-containing protein -
  DEFDS_RS13205 - 594154..594399 (-) 246 WP_231841030.1 hypothetical protein -
  DEFDS_RS13370 (DEFDS_0622) - 594424..595125 (-) 702 Protein_611 YhjD/YihY/BrkB family envelope integrity protein -
  DEFDS_RS03110 (DEFDS_0623) - 595208..595903 (+) 696 WP_041223586.1 PolC-type DNA polymerase III -
  DEFDS_RS03115 (DEFDS_0624) - 595884..597458 (-) 1575 WP_013007353.1 NFACT RNA binding domain-containing protein -
  DEFDS_RS03120 (DEFDS_0625) pilT 597461..598519 (-) 1059 WP_013007354.1 type IV pilus twitching motility protein PilT Machinery gene
  DEFDS_RS03125 (DEFDS_0626) - 598506..599585 (-) 1080 WP_013007355.1 hypothetical protein -
  DEFDS_RS03130 (DEFDS_0627) - 599578..600585 (-) 1008 WP_013007356.1 tetratricopeptide repeat protein -
  DEFDS_RS03135 (DEFDS_0628) - 601175..601552 (+) 378 WP_013007357.1 phage holin family protein -
  DEFDS_RS03140 (DEFDS_0629) - 601543..602052 (+) 510 WP_013007358.1 hypothetical protein -
  DEFDS_RS03145 (DEFDS_0630) - 602049..602336 (+) 288 WP_013007359.1 DUF1820 family protein -
  DEFDS_RS03150 (DEFDS_0631) - 602333..603367 (+) 1035 WP_013007360.1 LptF/LptG family permease -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39290.67 Da        Isoelectric Point: 7.8711

>NTDB_id=36638 DEFDS_RS03120 WP_013007354.1 597461..598519(-) (pilT) [Deferribacter desulfuricans SSM1]
MAKIDAFFKFLIEQGGSDLHLSAGCKPLMRVHGELVEIKYQTLTDELLRTLLFEIIDEKSKKEFLEKRDLDFAYEIKGLA
RFRANYFFQKRGIGAVFRQIPAKILSVEELGLPPQILKFADLSRGLVLVTGPTGSGKSTTLAAIIDYINRKRHDHILTIE
DPIEFVHVNKGCLVNQREVGNHTASFASALRAALREDPDVILVGEMRDLETIELAITAAETGHLVFGTLHTNSAAKTIDR
IIDAFPAGQQAQIRTMLSESLKGVVAQQLLKRCDKPGRVAALEILFVNSAVANLIREGKTFQIPSVIQTGKNEGMQLMDQ
AIMDLLMQKIVSPEEAYIKANDKKTFERFLNK

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=36638 DEFDS_RS03120 WP_013007354.1 597461..598519(-) (pilT) [Deferribacter desulfuricans SSM1]
ATGGCAAAGATTGATGCTTTTTTCAAATTTTTAATTGAACAAGGTGGAAGCGACCTTCATTTAAGCGCTGGTTGCAAACC
TCTAATGAGAGTTCACGGCGAGCTTGTAGAAATAAAGTATCAAACTTTGACTGATGAGCTTTTACGAACTCTTCTCTTTG
AAATAATAGACGAAAAATCAAAAAAAGAGTTTTTAGAAAAAAGAGATTTAGATTTTGCATATGAGATAAAAGGGTTAGCT
AGATTTAGGGCAAACTACTTTTTTCAAAAGAGAGGGATAGGAGCCGTTTTTAGACAAATACCGGCTAAGATTTTATCTGT
AGAAGAGTTAGGGTTGCCTCCTCAGATTTTGAAATTTGCAGACTTATCAAGGGGTTTAGTACTGGTCACTGGTCCCACAG
GAAGTGGTAAATCAACTACACTAGCTGCAATCATTGATTATATAAACAGAAAAAGACATGACCACATTTTAACAATTGAA
GATCCAATAGAGTTTGTACATGTAAATAAGGGGTGTTTAGTTAATCAAAGAGAAGTTGGAAATCATACTGCATCCTTTGC
CTCAGCCCTAAGGGCTGCGTTAAGAGAAGACCCAGATGTAATATTAGTTGGTGAGATGAGAGATCTTGAAACAATAGAAC
TGGCTATCACAGCAGCGGAAACAGGTCATCTTGTTTTTGGTACGCTTCACACAAACTCTGCAGCAAAAACTATAGATAGG
ATTATAGACGCCTTCCCAGCAGGGCAACAAGCTCAAATTAGAACGATGCTTTCAGAATCTTTAAAGGGTGTCGTTGCACA
ACAGCTTTTAAAAAGATGTGATAAGCCTGGTAGAGTAGCTGCATTAGAAATACTTTTTGTCAATAGTGCTGTAGCTAATT
TAATTAGAGAAGGTAAAACGTTTCAGATCCCTTCTGTAATTCAAACAGGAAAAAATGAAGGGATGCAACTCATGGATCAA
GCTATAATGGATTTACTTATGCAAAAAATTGTTTCACCAGAGGAAGCTTATATAAAAGCAAACGATAAAAAAACTTTTGA
GCGTTTTTTGAATAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(93-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

58.309

97.443

0.568
  pilT Legionella pneumophila strain ERS1305867

58.309

97.443

0.568
  pilT Acinetobacter baylyi ADP1

56.268

97.443

0.548
  pilT Pseudomonas aeruginosa PAK

55.977

97.443

0.545
  pilT Acinetobacter baumannii D1279779

55.685

97.443

0.543
  pilT Acinetobacter baumannii strain A118

55.685

97.443

0.543
  pilT Acinetobacter nosocomialis M2

55.685

97.443

0.543
  pilT Pseudomonas stutzeri DSM 10701

55.394

97.443

0.54
  pilT Neisseria meningitidis 8013

54.651

97.727

0.534
  pilT Neisseria gonorrhoeae MS11

54.36

97.727

0.531
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.07

97.727

0.528
  pilT Vibrio cholerae strain A1552

54.07

97.727

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.724

98.864

0.511
  pilU Pseudomonas stutzeri DSM 10701

41.593

96.307

0.401
  pilU Vibrio cholerae strain A1552

41.593

96.307

0.401
  pilU Acinetobacter baylyi ADP1

39.286

95.455

0.375

Multiple sequence alignment