Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THAL_RS05295 Genome accession   NC_013894
Coordinates   976901..977974 (+) Length   357 a.a.
NCBI ID   WP_012992077.1    Uniprot ID   D3SLP1
Organism   Thermocrinis albus DSM 14484     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 971901..982974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THAL_RS05280 (Thal_1036) - 972053..973069 (+) 1017 WP_012992074.1 DNA-processing protein DprA -
  THAL_RS05285 (Thal_1037) - 973062..973427 (-) 366 WP_012992075.1 hypothetical protein -
  THAL_RS08530 - 973424..975355 (-) 1932 WP_345775602.1 class I tRNA ligase family protein -
  THAL_RS08535 - 975701..976858 (-) 1158 Protein_1033 class I tRNA ligase family protein -
  THAL_RS05295 (Thal_1039) pilT 976901..977974 (+) 1074 WP_012992077.1 type IV pilus twitching motility protein PilT Machinery gene
  THAL_RS05300 (Thal_1040) - 977974..979197 (+) 1224 WP_012992078.1 type II secretion system F family protein -
  THAL_RS05305 (Thal_1041) hypE 979257..980255 (+) 999 WP_012992079.1 hydrogenase expression/formation protein HypE -
  THAL_RS05310 (Thal_1042) hypA 980257..980601 (+) 345 WP_012992080.1 hydrogenase maturation nickel metallochaperone HypA -
  THAL_RS05315 (Thal_1043) - 980605..980856 (+) 252 WP_012992081.1 HypC/HybG/HupF family hydrogenase formation chaperone -
  THAL_RS05320 (Thal_1044) hypD 980835..981971 (+) 1137 WP_012992082.1 hydrogenase formation protein HypD -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40277.63 Da        Isoelectric Point: 7.5094

>NTDB_id=36525 THAL_RS05295 WP_012992077.1 976901..977974(+) (pilT) [Thermocrinis albus DSM 14484]
MEDLLLTEILYKAVKLNASDVHITAGSRPALRIDGKITPLAEYPVLTPEQTQRMAYSVMSEKHRKTLEEKGQVDFSFGIK
DIGRFRSNVFFQRGSVAAVFRRLPNKIMSVKELGLSEKVLQLCHLSMGLVLVTGPTGSGKTTTLASLINYINENFPYHII
TIEDPIEYVFQHRKSIVNQREIGEDVQDFAQALRAALREDPDVILVGEMRDLETVEIALRAAETGHLVFATLHTNTAIST
ITRIIDIFPPEQQEQIRVQLSFVLQGVISQRLLPKIGGGRVLAYELLIPNVAIRNLIRENKLQQVYSLMQSGQAETGMQT
MNQSLYKLYRSGLITLEDAYRYSPDVKELEMMLKRAS

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=36525 THAL_RS05295 WP_012992077.1 976901..977974(+) (pilT) [Thermocrinis albus DSM 14484]
ATGGAGGATCTTCTGCTTACCGAGATCCTGTACAAGGCGGTAAAACTTAACGCCTCAGACGTGCACATAACGGCGGGCAG
CAGACCGGCACTGCGTATAGATGGTAAGATAACACCTCTGGCAGAGTACCCTGTCCTGACACCAGAGCAGACACAGAGGA
TGGCCTATTCGGTGATGTCGGAAAAGCACAGAAAGACTCTCGAAGAGAAGGGGCAGGTGGACTTCTCCTTCGGTATAAAG
GACATAGGTAGATTTAGATCCAACGTGTTTTTCCAAAGAGGATCAGTGGCGGCTGTTTTCAGAAGACTTCCCAACAAGAT
AATGAGTGTAAAAGAGCTTGGTCTCTCAGAGAAGGTGCTTCAGCTCTGTCACCTCAGTATGGGACTTGTTTTGGTGACAG
GACCAACGGGTAGCGGTAAGACCACCACACTGGCCAGCCTAATAAACTACATAAACGAGAACTTCCCCTATCACATAATC
ACCATAGAGGACCCTATAGAGTACGTTTTCCAGCACAGAAAGAGCATAGTGAATCAAAGAGAGATAGGCGAGGATGTGCA
GGACTTTGCACAGGCACTGAGAGCAGCTCTCCGTGAAGACCCAGACGTTATCTTGGTAGGTGAGATGCGAGACCTTGAGA
CGGTGGAGATAGCCCTTCGTGCTGCCGAAACGGGACACCTGGTCTTTGCCACACTTCACACCAACACAGCCATATCCACC
ATAACACGTATCATCGACATCTTTCCCCCCGAGCAACAGGAACAGATAAGGGTTCAGCTTTCCTTCGTGTTACAGGGTGT
TATATCTCAGAGATTACTTCCTAAAATAGGGGGTGGTAGGGTTCTTGCCTACGAACTACTTATCCCCAACGTCGCCATAC
GGAACCTCATAAGGGAAAACAAACTACAGCAGGTTTACTCTCTTATGCAGAGCGGACAGGCAGAGACAGGTATGCAGACC
ATGAATCAGTCCCTTTACAAACTCTACAGGTCTGGTCTCATAACTCTTGAAGATGCTTACAGGTACTCCCCCGATGTGAA
AGAGTTGGAGATGATGTTGAAGAGGGCCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3SLP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.858

98.88

0.493
  pilT Acinetobacter nosocomialis M2

52.096

93.557

0.487
  pilT Acinetobacter baumannii strain A118

52.096

93.557

0.487
  pilT Acinetobacter baumannii D1279779

52.096

93.557

0.487
  pilT Neisseria meningitidis 8013

50.145

96.639

0.485
  pilT Neisseria gonorrhoeae MS11

49.855

96.639

0.482
  pilT Acinetobacter baylyi ADP1

51.198

93.557

0.479
  pilT Pseudomonas aeruginosa PAK

50.898

93.557

0.476
  pilT Legionella pneumophila strain Lp02

50.746

93.838

0.476
  pilT Legionella pneumophila strain ERS1305867

50.746

93.838

0.476
  pilT Pseudomonas stutzeri DSM 10701

49.701

93.557

0.465
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.249

93.277

0.459
  pilT Vibrio cholerae strain A1552

49.249

93.277

0.459
  pilU Pseudomonas stutzeri DSM 10701

40.741

98.319

0.401
  pilU Vibrio cholerae strain A1552

40.533

94.678

0.384
  pilU Acinetobacter baylyi ADP1

38.746

98.319

0.381

Multiple sequence alignment