Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EYS05_RS14315 Genome accession   NZ_CP040518
Coordinates   3078554..3079627 (+) Length   357 a.a.
NCBI ID   WP_138277411.1    Uniprot ID   -
Organism   Blautia sp. SC05B48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3073554..3084627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYS05_RS14290 (EYS05_14290) - 3073694..3074704 (+) 1011 WP_138277409.1 uroporphyrinogen decarboxylase family protein -
  EYS05_RS14305 (EYS05_14305) ltrA 3075229..3076620 (+) 1392 WP_138277696.1 group II intron reverse transcriptase/maturase -
  EYS05_RS14310 (EYS05_14310) - 3076838..3078529 (+) 1692 WP_138277410.1 ATPase, T2SS/T4P/T4SS family -
  EYS05_RS14315 (EYS05_14315) pilT 3078554..3079627 (+) 1074 WP_138277411.1 type IV pilus twitching motility protein PilT Machinery gene
  EYS05_RS14320 (EYS05_14320) - 3079641..3080855 (+) 1215 WP_138277412.1 type II secretion system F family protein -
  EYS05_RS14325 (EYS05_14325) - 3080987..3081502 (+) 516 WP_243119123.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EYS05_RS14330 (EYS05_14330) - 3081536..3082294 (+) 759 WP_138277414.1 A24 family peptidase -
  EYS05_RS14335 (EYS05_14335) pilM 3082448..3084088 (+) 1641 WP_138277415.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39768.65 Da        Isoelectric Point: 5.4417

>NTDB_id=364928 EYS05_RS14315 WP_138277411.1 3078554..3079627(+) (pilT) [Blautia sp. SC05B48]
MYILDEVLSGSVRVKASDVHFTVGRPPVYRVDGQLDPIEGEALSPEMMEKLLMPLIDVRHRAQLQENGQTDFAYAISGVG
RFRVNVFKQRGTLAAAMRCLPFGIPEPGSLGIPPEVVEMTTRKRGLVLVTGPTGSGKSTTLASLIHEINKKYCYHIITLE
DPIEYLHRHDRSIVNQREIGSDSMDYAQALRAALREDPDVILVGEMRDLETISTAITAAETGHLVFSTLHTTGADQTLDR
IIDVFPPDQQQQIRIQLAAVLECIVSQQLLKRADGQGRVAALEVLFANNAIRNLIREGKTYQIPSVMQTSKRLGMQTMDD
ALYDLYMRKIIDADEAVTYAQDPVSMNKKVTFDLPDF

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=364928 EYS05_RS14315 WP_138277411.1 3078554..3079627(+) (pilT) [Blautia sp. SC05B48]
ATGTACATATTAGATGAAGTATTATCAGGGTCGGTCCGTGTGAAGGCATCGGATGTCCACTTTACTGTGGGCAGGCCGCC
GGTGTATCGTGTGGATGGACAGCTGGATCCCATAGAAGGAGAAGCACTGTCACCTGAAATGATGGAAAAGCTACTCATGC
CTCTGATCGATGTAAGACACAGAGCGCAGCTGCAGGAAAACGGGCAGACTGATTTCGCATACGCCATATCCGGGGTAGGC
CGTTTTCGAGTGAATGTGTTTAAGCAGAGAGGAACGCTGGCAGCAGCCATGCGGTGTCTGCCTTTTGGTATCCCGGAACC
GGGATCACTGGGGATCCCACCGGAAGTGGTAGAAATGACTACAAGGAAAAGAGGCCTTGTACTTGTTACAGGACCCACAG
GAAGCGGTAAATCCACAACGCTGGCATCACTGATCCATGAGATCAACAAAAAGTATTGTTATCATATCATTACGCTGGAG
GATCCCATTGAGTATCTGCACAGGCATGACCGGTCCATTGTAAATCAGAGAGAGATCGGAAGTGATTCCATGGATTACGC
ACAGGCTCTCCGTGCGGCACTACGCGAAGATCCGGACGTGATCCTGGTGGGCGAGATGCGTGATCTGGAAACGATCTCTA
CAGCTATCACTGCGGCGGAGACCGGGCATCTGGTGTTTTCCACGCTGCATACCACAGGAGCAGATCAGACCCTTGACCGG
ATCATCGATGTTTTTCCGCCGGATCAGCAACAGCAGATCCGTATCCAGCTTGCGGCTGTACTGGAATGCATTGTATCCCA
GCAGCTTTTGAAGCGGGCAGACGGACAGGGGCGTGTGGCTGCCCTTGAGGTGCTGTTTGCAAACAATGCCATCCGGAATC
TGATCCGCGAGGGCAAAACATATCAGATCCCATCGGTCATGCAGACCAGCAAAAGACTGGGAATGCAGACCATGGATGAT
GCGTTGTATGATCTCTATATGAGAAAAATCATCGATGCAGATGAAGCCGTTACCTATGCACAGGATCCGGTATCCATGAA
CAAAAAAGTAACCTTTGATCTGCCTGATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.744

91.877

0.485
  pilT Legionella pneumophila strain Lp02

49.849

92.717

0.462
  pilT Legionella pneumophila strain ERS1305867

49.849

92.717

0.462
  pilT Neisseria meningitidis 8013

47.55

97.199

0.462
  pilT Neisseria gonorrhoeae MS11

47.262

97.199

0.459
  pilT Acinetobacter baylyi ADP1

46.647

96.078

0.448
  pilT Acinetobacter nosocomialis M2

46.356

96.078

0.445
  pilT Acinetobacter baumannii D1279779

46.356

96.078

0.445
  pilT Acinetobacter baumannii strain A118

46.356

96.078

0.445
  pilT Pseudomonas stutzeri DSM 10701

46.765

95.238

0.445
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.886

98.599

0.443
  pilT Vibrio cholerae strain A1552

44.886

98.599

0.443
  pilT Pseudomonas aeruginosa PAK

45.882

95.238

0.437
  pilU Vibrio cholerae strain A1552

42.136

94.398

0.398
  pilU Pseudomonas stutzeri DSM 10701

41.543

94.398

0.392
  pilU Acinetobacter baylyi ADP1

39.655

97.479

0.387

Multiple sequence alignment