Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   XBJ1_RS14225 Genome accession   NC_013892
Coordinates   3263506..3264516 (-) Length   336 a.a.
NCBI ID   WP_012989698.1    Uniprot ID   D3V480
Organism   Xenorhabdus bovienii SS-2004     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3258506..3269516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XBJ1_RS14195 (XBJ1_3335) - 3259000..3259815 (-) 816 WP_012989692.1 MBL fold metallo-hydrolase -
  XBJ1_RS14200 (XBJ1_3336) - 3259890..3260357 (-) 468 WP_230578790.1 A24 family peptidase -
  XBJ1_RS14205 (XBJ1_3337) rsmE 3260700..3261431 (+) 732 WP_012989694.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  XBJ1_RS14210 (XBJ1_3338) gshB 3261441..3262400 (+) 960 WP_012989695.1 glutathione synthase -
  XBJ1_RS14215 (XBJ1_3339) - 3262492..3263055 (+) 564 WP_012989696.1 YqgE/AlgH family protein -
  XBJ1_RS14220 (XBJ1_3340) ruvX 3263055..3263480 (+) 426 WP_012989697.1 Holliday junction resolvase RuvX -
  XBJ1_RS14225 (XBJ1_3341) pilT 3263506..3264516 (-) 1011 WP_012989698.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  XBJ1_RS14230 (XBJ1_3342) - 3264538..3265239 (+) 702 WP_012989699.1 YggS family pyridoxal phosphate-dependent enzyme -
  XBJ1_RS14235 (XBJ1_3343) proC 3265283..3266104 (+) 822 WP_012989700.1 pyrroline-5-carboxylate reductase -
  XBJ1_RS14240 (XBJ1_3344) - 3266138..3266701 (+) 564 WP_012989701.1 YggT family protein -
  XBJ1_RS14245 (XBJ1_3345) - 3266732..3267325 (+) 594 WP_012989702.1 XTP/dITP diphosphatase -
  XBJ1_RS14250 (XBJ1_3346) hemW 3267318..3268448 (+) 1131 WP_012989703.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37451.19 Da        Isoelectric Point: 7.0912

>NTDB_id=36478 XBJ1_RS14225 WP_012989698.1 3263506..3264516(-) (pilT) [Xenorhabdus bovienii SS-2004]
MNMEKLVALSVKHNASDLHLCVGKVPVLRINGVLYPQMQLAEMQGDTLIAWSRKILTEEQQQQLQEYGHVDFVVEMPDKQ
RFRGNLFYQQCHVSLVLRLIADKCPTLEQLYAPDIIQRLILQEQSGLVLVTGATGSGKSTTLSAIINAFNNHGNKHIITL
EDPIEFVHQSRNCLIQQRQISRDVPDYQTALKGALRQDPDVILLGELRDKETIRLALTAAETGHLVLSTLHTRGAIQALD
RLVDVFSAEEKGWICSQLAGSLKAVISQQLLPAREGGRVAIYEVLVVNQAVSHLIREGKNHQIATLMQTGAAYGMQTHEQ
SRQQRELLGLLAVVPQ

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=36478 XBJ1_RS14225 WP_012989698.1 3263506..3264516(-) (pilT) [Xenorhabdus bovienii SS-2004]
ATGAATATGGAAAAATTGGTGGCTCTTAGTGTAAAGCATAATGCTTCTGATCTGCATCTTTGTGTCGGGAAAGTTCCGGT
TTTGCGCATCAATGGTGTGCTGTACCCACAGATGCAACTGGCAGAAATGCAGGGTGATACCCTCATTGCATGGAGTAGGA
AAATATTGACCGAAGAACAACAGCAGCAGTTACAGGAATACGGTCATGTTGATTTTGTGGTGGAAATGCCAGATAAGCAG
CGGTTCAGGGGGAATCTGTTTTACCAACAGTGTCATGTTTCATTGGTATTGAGGTTAATCGCGGATAAATGTCCTACTCT
GGAACAATTATATGCACCGGACATTATCCAACGTCTGATTTTGCAGGAGCAAAGCGGGTTGGTATTAGTAACAGGCGCAA
CGGGAAGCGGTAAATCTACTACTTTGTCGGCGATAATCAATGCGTTCAATAATCATGGCAACAAACATATCATTACGTTG
GAAGACCCAATAGAATTCGTTCATCAAAGCCGAAATTGCCTGATACAGCAAAGACAAATCAGCAGGGATGTGCCGGATTA
TCAAACGGCTTTGAAAGGTGCATTACGGCAAGATCCTGATGTGATTTTGCTGGGAGAATTGCGTGACAAAGAGACGATCC
GCTTGGCATTGACAGCGGCTGAAACAGGACATCTGGTTCTGTCAACATTACATACCCGTGGGGCAATTCAAGCACTAGAT
CGTTTGGTTGATGTTTTCAGTGCAGAAGAAAAGGGCTGGATATGCAGCCAACTGGCAGGAAGTTTAAAAGCAGTTATTTC
TCAACAACTGCTTCCTGCCAGAGAAGGGGGAAGAGTAGCCATTTATGAAGTTCTGGTCGTCAACCAAGCGGTCAGCCATT
TGATCAGGGAAGGAAAAAATCATCAAATCGCGACATTAATGCAGACAGGAGCTGCCTACGGTATGCAAACTCATGAGCAA
AGCCGACAACAACGTGAGTTGTTGGGATTGCTGGCTGTTGTTCCACAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3V480

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.309

96.429

0.485
  pilT Vibrio cholerae strain A1552

50.309

96.429

0.485
  pilT Neisseria gonorrhoeae MS11

48.949

99.107

0.485
  pilT Neisseria meningitidis 8013

48.949

99.107

0.485
  pilT Acinetobacter baumannii D1279779

46.608

100

0.47
  pilT Acinetobacter baumannii strain A118

46.608

100

0.47
  pilT Pseudomonas stutzeri DSM 10701

47.289

98.81

0.467
  pilT Acinetobacter baylyi ADP1

48.148

96.429

0.464
  pilT Acinetobacter nosocomialis M2

47.352

95.536

0.452
  pilT Pseudomonas aeruginosa PAK

47.003

94.345

0.443
  pilT Legionella pneumophila strain ERS1305867

45.938

95.238

0.438
  pilT Legionella pneumophila strain Lp02

45.938

95.238

0.438
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.062

95.238

0.42
  pilU Pseudomonas stutzeri DSM 10701

40.683

95.833

0.39
  pilU Acinetobacter baylyi ADP1

40.557

96.131

0.39
  pilU Vibrio cholerae strain A1552

40

92.262

0.369

Multiple sequence alignment