Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FDY98_RS02090 Genome accession   NZ_CP040451
Coordinates   441622..442665 (+) Length   347 a.a.
NCBI ID   WP_027958270.1    Uniprot ID   A0A6B8QL65
Organism   Halomonas sp. PA16-9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436622..447665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDY98_RS02060 (FDY98_02070) - 436818..437267 (-) 450 WP_027958265.1 DUF4426 domain-containing protein -
  FDY98_RS02065 (FDY98_02075) metW 437307..437900 (-) 594 WP_027958266.1 methionine biosynthesis protein MetW -
  FDY98_RS02070 (FDY98_02080) - 437900..439126 (-) 1227 WP_009287944.1 homoserine O-acetyltransferase -
  FDY98_RS02075 (FDY98_02085) - 439344..439937 (-) 594 WP_009287945.1 YggT family protein -
  FDY98_RS02080 (FDY98_02090) proC 439993..440817 (-) 825 WP_027958268.1 pyrroline-5-carboxylate reductase -
  FDY98_RS02085 (FDY98_02095) - 440873..441589 (-) 717 WP_027958269.1 YggS family pyridoxal phosphate-dependent enzyme -
  FDY98_RS02090 (FDY98_02100) pilT 441622..442665 (+) 1044 WP_027958270.1 type IV pilus twitching motility protein PilT Machinery gene
  FDY98_RS02095 (FDY98_02105) - 442892..444031 (-) 1140 WP_027958271.1 anhydro-N-acetylmuramic acid kinase -
  FDY98_RS02100 (FDY98_02110) tyrS 444177..445376 (+) 1200 WP_009287950.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38199.71 Da        Isoelectric Point: 6.0072

>NTDB_id=364572 FDY98_RS02090 WP_027958270.1 441622..442665(+) (pilT) [Halomonas sp. PA16-9]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAIDNNDVRRLIYAIMNDRQRRDYEEHLEIDFSFEVLGIAR
FRVNAFTQARGAGAVFRTIPNQVLSIQTLGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYEHILTIED
PVEFVHVSKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAVKTIDRI
IDVFPSEEKAMVRSMLSESLQAVVSQSLLKRQGGGRVAAHEILMATSAVRNLIREDKVAQMYSAIQTGGSLGMQTLDAAL
SRLVNEGVVSIEEAQAQAKGTLAAKDE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=364572 FDY98_RS02090 WP_027958270.1 441622..442665(+) (pilT) [Halomonas sp. PA16-9]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCCTCTGACTTGCACCTTTCGGCCGGTTTACCGCCCAT
GATACGTGTTGATGGCGATATTCGTCGGCTCAATGTGCCCGCTATCGATAATAACGATGTGCGCAGATTGATCTACGCCA
TCATGAATGATCGTCAGCGGCGCGACTACGAAGAGCACCTGGAAATTGATTTTTCCTTTGAAGTGCTGGGGATCGCCCGC
TTTCGTGTTAACGCCTTTACTCAAGCTCGCGGGGCGGGGGCGGTGTTTCGCACCATTCCCAATCAAGTACTTTCCATACA
AACCCTTGGGCTCGGTGAGGTGTTCGAGCGCCTGGCGATGTTACCGCGGGGCCTGGTGCTGGTGACTGGGCCAACGGGGT
CGGGCAAAAGCACCACGCTGGCCGCCATGATCGATTACATTAACGATCATCGTTACGAGCACATTCTCACCATTGAAGAC
CCCGTCGAGTTTGTCCACGTTAGCAAGCGCTGTTTGATTAACCAACGCGAAGTGCATCGCGACACCCATAGCTTTGCCGA
TGCGTTGCGTAGCGCGCTGCGTGAAGACCCGGATGTGATTCTGGTCGGCGAGCTGCGTGATCTGGAGACTATCCGCTTAG
CACTGACCGCTGCTGAAACCGGTCATCTGGTGCTGGGAACGCTGCATACCACCTCTGCCGTCAAGACAATCGACCGGATC
ATCGATGTTTTTCCCAGCGAAGAGAAAGCCATGGTGCGCTCCATGCTTTCGGAATCGCTCCAGGCAGTGGTGTCGCAATC
GCTGCTTAAACGTCAGGGCGGCGGGCGCGTAGCGGCCCACGAAATCCTCATGGCCACTTCTGCAGTGCGCAACTTGATCC
GCGAAGACAAAGTGGCACAGATGTACTCAGCCATTCAAACCGGTGGTAGCCTGGGTATGCAAACGCTGGATGCCGCGCTG
TCTCGGTTAGTCAATGAGGGCGTTGTAAGCATAGAGGAAGCCCAGGCTCAGGCTAAAGGCACTCTCGCCGCAAAAGATGA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B8QL65

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.696

97.695

0.749
  pilT Pseudomonas stutzeri DSM 10701

76.106

97.695

0.744
  pilT Acinetobacter nosocomialis M2

74.926

97.695

0.732
  pilT Acinetobacter baumannii D1279779

74.926

97.695

0.732
  pilT Acinetobacter baumannii strain A118

74.926

97.695

0.732
  pilT Acinetobacter baylyi ADP1

74.041

97.695

0.723
  pilT Legionella pneumophila strain Lp02

71.893

97.406

0.7
  pilT Legionella pneumophila strain ERS1305867

71.893

97.406

0.7
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.605

99.135

0.68
  pilT Vibrio cholerae strain A1552

68.605

99.135

0.68
  pilT Neisseria meningitidis 8013

64.327

98.559

0.634
  pilT Neisseria gonorrhoeae MS11

64.035

98.559

0.631
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.982

97.695

0.527
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.542

0.412
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401
  pilU Acinetobacter baylyi ADP1

40.294

97.983

0.395

Multiple sequence alignment