Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KVAR_RS03480 Genome accession   NC_013850
Coordinates   726523..727503 (+) Length   326 a.a.
NCBI ID   WP_012967228.1    Uniprot ID   -
Organism   Klebsiella variicola At-22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721523..732503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KVAR_RS03445 (Kvar_0690) - 722059..722385 (+) 327 WP_012967224.1 YggL family protein -
  KVAR_RS03450 (Kvar_0691) - 722436..723155 (+) 720 WP_008806471.1 DUF2884 domain-containing protein -
  KVAR_RS03455 (Kvar_0692) hemW 723196..724332 (-) 1137 WP_012967226.1 radical SAM family heme chaperone HemW -
  KVAR_RS03460 (Kvar_0693) - 724325..724918 (-) 594 WP_012967227.1 XTP/dITP diphosphatase -
  KVAR_RS03465 (Kvar_0694) yggU 724931..725221 (-) 291 WP_008806468.1 DUF167 family protein YggU -
  KVAR_RS03470 (Kvar_0695) - 725218..725784 (-) 567 WP_004205182.1 YggT family protein -
  KVAR_RS03475 (Kvar_0696) - 725805..726506 (-) 702 WP_008806467.1 YggS family pyridoxal phosphate-dependent enzyme -
  KVAR_RS03480 (Kvar_0697) pilT 726523..727503 (+) 981 WP_012967228.1 type IV pilus twitching motility protein PilT Machinery gene
  KVAR_RS03485 (Kvar_0698) - 727852..728505 (+) 654 WP_004205179.1 LuxR family transcriptional regulator -
  KVAR_RS03490 (Kvar_0699) ruvX 728558..728974 (-) 417 WP_012540565.1 Holliday junction resolvase RuvX -
  KVAR_RS03495 (Kvar_0700) - 728974..729537 (-) 564 WP_012540566.1 YqgE/AlgH family protein -
  KVAR_RS03500 (Kvar_0701) gshB 729712..730662 (-) 951 WP_012967229.1 glutathione synthase -
  KVAR_RS03505 (Kvar_0702) rsmE 730683..731414 (-) 732 WP_012967230.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KVAR_RS03510 (Kvar_0703) endA 731506..732213 (-) 708 WP_008806460.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35419.97 Da        Isoelectric Point: 6.6816

>NTDB_id=36234 KVAR_RS03480 WP_012967228.1 726523..727503(+) (pilT) [Klebsiella variicola At-22]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLEPAPFPAPDIPDLLNCWLDAAQLQHWQEQGQIDFALTLACGLRLRA
SAFAHTRGISLVLRLLPEQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGIMQTGMQAGMLTFTQSFQQR
VAAGEL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=36234 KVAR_RS03480 WP_012967228.1 726523..727503(+) (pilT) [Klebsiella variicola At-22]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGCCCGCCCCGTTTCCCGCGCCGGATATCCCGGATTTACTCAACTGCTGGCTTGATG
CCGCACAGTTACAGCATTGGCAGGAACAGGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTTTGCGGCTGCGCGCC
AGCGCTTTCGCCCATACGCGTGGAATATCGCTGGTGCTACGCCTTCTGCCTGAACAGTGCCCGCGTCTGGAGACGCTGGG
CGCCCCGCCTGCGCTGAGCGAGCTGCTGGCGGAAGAGAGTGGTTTGCTGCTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTGGGGCATCTCAACCAGCATCTTGATGGCCATATTCTGACCCTGGAGGACCCGGTGGAG
TTTATCCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAGATTGGCCGTCATTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGGGACAGCGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAGACCGGGCATCTGGTGATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAAAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGTAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTGCC
TGCACGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTTTGGTCAATACCCCCGCAGTAGCTAACTTGATCCGTGAAGGAA
AAGTGCATCAGTTGCCCGGCATAATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACGCAGAGTTTTCAGCAACGT
GTCGCCGCAGGCGAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.988

100

0.521
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.988

100

0.521
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Legionella pneumophila strain ERS1305867

47.531

99.387

0.472
  pilT Legionella pneumophila strain Lp02

47.531

99.387

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.849

97.239

0.426
  pilU Vibrio cholerae strain A1552

40.426

100

0.408
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.405
  pilU Acinetobacter baylyi ADP1

39.252

98.466

0.386

Multiple sequence alignment