Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FDN13_RS02810 Genome accession   NZ_CP040093
Coordinates   529871..530929 (-) Length   352 a.a.
NCBI ID   WP_138978801.1    Uniprot ID   A0A5B7TGT5
Organism   Caloramator sp. E03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 524871..535929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDN13_RS02770 (FDN13_02770) - 524902..525510 (-) 609 WP_138978794.1 hypothetical protein -
  FDN13_RS02775 (FDN13_02775) - 525522..525929 (-) 408 WP_138978795.1 hypothetical protein -
  FDN13_RS02780 (FDN13_02780) - 525922..526332 (-) 411 WP_138978796.1 hypothetical protein -
  FDN13_RS02785 (FDN13_02785) - 526343..526744 (-) 402 WP_138978797.1 type II secretion system protein -
  FDN13_RS02790 (FDN13_02790) - 526741..527235 (-) 495 WP_168190048.1 GspH/FimT family pseudopilin -
  FDN13_RS02795 (FDN13_02795) - 527465..528211 (-) 747 WP_138981004.1 A24 family peptidase -
  FDN13_RS02800 (FDN13_02800) - 528238..528606 (-) 369 WP_168190049.1 type II secretion system protein -
  FDN13_RS02805 (FDN13_02805) - 528661..529857 (-) 1197 WP_138978800.1 type II secretion system F family protein -
  FDN13_RS02810 (FDN13_02810) pilT 529871..530929 (-) 1059 WP_138978801.1 type IV pilus twitching motility protein PilT Machinery gene
  FDN13_RS02815 (FDN13_02815) - 530892..532121 (-) 1230 WP_138978802.1 GspE/PulE family protein -
  FDN13_RS02820 (FDN13_02820) aroE 532153..532983 (-) 831 WP_138978803.1 shikimate dehydrogenase -
  FDN13_RS02825 (FDN13_02825) - 532970..533491 (-) 522 WP_138978804.1 YqeG family HAD IIIA-type phosphatase -
  FDN13_RS02830 (FDN13_02830) sigK 533640..534314 (-) 675 WP_138981005.1 RNA polymerase sporulation sigma factor SigK -
  FDN13_RS02835 (FDN13_02835) udk 534474..535109 (-) 636 WP_138978805.1 uridine kinase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39294.54 Da        Isoelectric Point: 7.1605

>NTDB_id=362014 FDN13_RS02810 WP_138978801.1 529871..530929(-) (pilT) [Caloramator sp. E03]
MIPFQELLAKTVELNASDLHITVGIPPTMRINGNLMPYGNERLTPKEVEEYTKYVLTEEQYQKYISIGEIDLSYSVQGIG
RFRVNIFRQRGSDAMALRTVALQMPTLEKLNMPPVIKNLCKKTRGLILVTGPTGSGKSTTLAAMINEINNTRSGHIITLE
DPIEYLHKHKKSIVNQREIGYDTKSYSAALRSALREDPDVILVGEMRDLETISIAITAAETGHLVMSTLHTIGASKTIDR
IVDVFPPHQQQQIKVQLSAVIEGIISQQLITRADGEGRVCALEIMVATPAIRNLIREGKTHQIDSAVQTGGKYGMKTMDM
SLVELYKKGIITYDDAMTYCVDKDIMERLINF

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=362014 FDN13_RS02810 WP_138978801.1 529871..530929(-) (pilT) [Caloramator sp. E03]
ATGATACCATTTCAAGAATTATTAGCAAAAACAGTTGAACTTAATGCTTCAGATTTACACATTACTGTTGGTATACCACC
AACTATGAGAATAAATGGAAATCTGATGCCTTATGGAAATGAAAGGTTAACACCAAAGGAAGTTGAAGAATATACAAAAT
ATGTTCTTACTGAGGAGCAGTATCAAAAATATATTTCTATCGGAGAAATAGATCTTTCATATTCTGTTCAGGGCATTGGT
AGGTTTAGGGTAAATATTTTTAGACAGAGAGGTAGTGATGCTATGGCACTTAGAACCGTAGCACTGCAAATGCCTACCTT
AGAAAAACTTAATATGCCCCCTGTTATTAAAAACCTTTGTAAAAAAACAAGAGGTTTAATACTTGTTACTGGTCCTACAG
GAAGTGGAAAATCAACTACACTTGCAGCTATGATAAACGAAATTAACAATACAAGAAGCGGACATATAATTACGCTTGAA
GACCCTATTGAATATCTGCATAAACATAAAAAAAGTATAGTTAATCAAAGAGAAATTGGATATGATACAAAGTCATATTC
TGCAGCTTTAAGATCAGCTTTAAGAGAAGATCCTGATGTTATTCTTGTAGGAGAGATGAGAGATCTTGAAACTATTTCTA
TTGCAATAACTGCAGCTGAAACAGGACATCTTGTAATGTCAACGCTTCATACTATAGGGGCTTCGAAGACTATTGATAGG
ATAGTTGATGTTTTTCCTCCTCATCAGCAGCAACAGATAAAGGTACAGCTTTCAGCAGTTATTGAAGGAATAATATCTCA
ACAACTCATTACAAGAGCAGACGGAGAAGGAAGAGTTTGTGCTCTTGAAATAATGGTTGCAACACCTGCAATTAGAAATC
TTATTAGGGAAGGTAAAACACATCAGATAGATTCAGCAGTTCAAACAGGAGGGAAATATGGAATGAAAACTATGGATATG
TCTCTTGTTGAACTTTATAAAAAAGGTATTATAACTTATGATGATGCAATGACCTATTGTGTTGATAAGGATATAATGGA
GAGGCTTATCAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B7TGT5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.964

94.034

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.286

99.432

0.48
  pilT Acinetobacter baylyi ADP1

51.84

92.614

0.48
  pilT Legionella pneumophila strain Lp02

49.267

96.875

0.477
  pilT Legionella pneumophila strain ERS1305867

49.267

96.875

0.477
  pilT Pseudomonas stutzeri DSM 10701

50.755

94.034

0.477
  pilT Neisseria meningitidis 8013

48.68

96.875

0.472
  pilT Acinetobacter baumannii strain A118

49.55

94.602

0.469
  pilT Acinetobacter nosocomialis M2

49.55

94.602

0.469
  pilT Acinetobacter baumannii D1279779

49.55

94.602

0.469
  pilT Vibrio cholerae strain A1552

50.459

92.898

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

92.898

0.469
  pilT Neisseria gonorrhoeae MS11

48.387

96.875

0.469
  pilU Pseudomonas stutzeri DSM 10701

43.323

95.739

0.415
  pilU Vibrio cholerae strain A1552

41.615

91.477

0.381
  pilU Acinetobacter baylyi ADP1

39.645

96.023

0.381

Multiple sequence alignment