Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FBF69_RS06045 Genome accession   NZ_CP039453
Coordinates   1216676..1217656 (+) Length   326 a.a.
NCBI ID   WP_136485043.1    Uniprot ID   -
Organism   Enterobacter bugandensis strain 220     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1211676..1222656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBF69_RS06015 (FAI36_06020) - 1212056..1213198 (-) 1143 WP_420898904.1 IS481 family transposase -
  FBF69_RS06020 (FAI36_06025) hemW 1213344..1214486 (-) 1143 WP_136485039.1 radical SAM family heme chaperone HemW -
  FBF69_RS06025 (FAI36_06030) - 1214479..1215072 (-) 594 WP_136485041.1 XTP/dITP diphosphatase -
  FBF69_RS06030 (FAI36_06035) yggU 1215076..1215372 (-) 297 WP_047364545.1 DUF167 family protein YggU -
  FBF69_RS06035 (FAI36_06040) - 1215369..1215935 (-) 567 WP_045260768.1 YggT family protein -
  FBF69_RS06040 (FAI36_06045) - 1215957..1216658 (-) 702 WP_047367421.1 YggS family pyridoxal phosphate-dependent enzyme -
  FBF69_RS06045 (FAI36_06050) pilT 1216676..1217656 (+) 981 WP_136485043.1 type IV pilus twitching motility protein PilT Machinery gene
  FBF69_RS06050 (FAI36_06055) ruvX 1217666..1218082 (-) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  FBF69_RS06055 (FAI36_06060) - 1218082..1218642 (-) 561 WP_028014368.1 YqgE/AlgH family protein -
  FBF69_RS06060 (FAI36_06065) gshB 1218717..1219664 (-) 948 WP_136485045.1 glutathione synthase -
  FBF69_RS06065 (FAI36_06070) rsmE 1219684..1220415 (-) 732 WP_041908327.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FBF69_RS06070 (FAI36_06075) endA 1220467..1221174 (-) 708 WP_028014365.1 deoxyribonuclease I -
  FBF69_RS06075 (FAI36_06080) - 1221269..1221766 (-) 498 WP_136485047.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35618.66 Da        Isoelectric Point: 6.4128

>NTDB_id=358734 FBF69_RS06045 WP_136485043.1 1216676..1217656(+) (pilT) [Enterobacter bugandensis strain 220]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPHAIPELLSSENGLILVTGATGSGKSTTLAAMVDFLNRHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLHDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=358734 FBF69_RS06045 WP_136485043.1 1216676..1217656(+) (pilT) [Enterobacter bugandensis strain 220]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCCGATGTCGGGGCGTTGCTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCCGTTACCCTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCGCTTGG
GGTGCCGCACGCTATTCCCGAACTGTTGTCCAGCGAAAACGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCAATGGTCGACTTCCTCAACCGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGGGCAGCACAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTGCTTCA
CGATCTTCAGGGCGGGCGCGTCGCGCTGTACGAGCTGCTGGTAAACACCGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
AGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain Lp02

49.689

98.773

0.491
  pilT Legionella pneumophila strain ERS1305867

49.689

98.773

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362

Multiple sequence alignment