Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   E8M03_RS08250 Genome accession   NZ_CP039448
Coordinates   1662309..1662755 (-) Length   148 a.a.
NCBI ID   WP_001796473.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VGC1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1657309..1667755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M03_RS08220 (E8M03_08310) gcvPA 1658235..1659581 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E8M03_RS08225 (E8M03_08315) gcvT 1659601..1660692 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  E8M03_RS08230 (E8M03_08320) - 1660851..1661375 (-) 525 WP_001015116.1 shikimate kinase -
  E8M03_RS08235 (E8M03_08325) - 1661365..1661511 (-) 147 WP_001789879.1 hypothetical protein -
  E8M03_RS08240 (E8M03_08330) comGF 1661608..1662105 (-) 498 WP_001838632.1 competence type IV pilus minor pilin ComGF Machinery gene
  E8M03_RS08245 (E8M03_08335) comGE 1662023..1662322 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E8M03_RS08250 (E8M03_08340) comGD 1662309..1662755 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  E8M03_RS08255 (E8M03_08345) comGC 1662733..1663044 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E8M03_RS08260 (E8M03_08350) comGB 1663058..1664128 (-) 1071 WP_000776407.1 competence type IV pilus assembly protein ComGB Machinery gene
  E8M03_RS08265 (E8M03_08355) comGA 1664100..1665074 (-) 975 WP_000697218.1 competence type IV pilus ATPase ComGA Machinery gene
  E8M03_RS08270 (E8M03_08360) - 1665126..1665749 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E8M03_RS08275 (E8M03_08365) - 1665746..1666075 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E8M03_RS08280 (E8M03_08370) - 1666075..1667061 (-) 987 WP_000161315.1 ROK family glucokinase -
  E8M03_RS08285 (E8M03_08375) - 1667058..1667261 (-) 204 WP_000087561.1 YqgQ family protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17131.31 Da        Isoelectric Point: 10.3312

>NTDB_id=358670 E8M03_RS08250 WP_001796473.1 1662309..1662755(-) (comGD) [Staphylococcus aureus strain VGC1]
MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVIDDEANIISLITELNYIKSQAIANQGYINVRFYENSD
TIKVIENNKIRFLKLKVGKIINVAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL

Nucleotide


Download         Length: 447 bp        

>NTDB_id=358670 E8M03_RS08250 WP_001796473.1 1662309..1662755(-) (comGD) [Staphylococcus aureus strain VGC1]
ATGGAGAAGCAGTTGCAAATTAGAAAGCAGTCAGCATTTACTATGATTGAGATGCTTGTGGTAATGATGTTAATCAGTAT
ATTTTTACTTTTGACAATGACATCTAAAGGATTAAGCAATCTTAGAGTAATAGATGATGAGGCAAATATCATTTCTCTTA
TTACTGAATTGAATTATATTAAGTCGCAAGCTATAGCAAATCAAGGATATATCAATGTTAGATTTTATGAAAACAGTGAC
ACTATTAAAGTAATAGAGAATAATAAAATACGATTTCTAAAATTAAAAGTAGGCAAAATAATTAATGTTGCAAAAGTTGA
TATTATTGCCTTTGATAAAAAAGGGAATATCAATAAATTTGGTAGCATAACAATTGATAATAACAATTCAATTTATAGAA
TAATATTCCATATTGAAAAAGGAAGAATTCGTTATGAAAAGCTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Staphylococcus aureus MW2

99.324

100

0.993
  comGD Staphylococcus aureus N315

99.324

100

0.993

Multiple sequence alignment