Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CB0101_RS08470 Genome accession   NZ_CP039373
Coordinates   1570953..1572035 (+) Length   360 a.a.
NCBI ID   WP_010312632.1    Uniprot ID   A0A4P7ZUN7
Organism   Synechococcus sp. CB0101     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1565953..1577035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CB0101_RS08450 (CB0101_08435) - 1566874..1567110 (-) 237 WP_010312642.1 sulfurtransferase TusA family protein -
  CB0101_RS08455 (CB0101_08440) dnaJ 1567118..1568248 (-) 1131 WP_010312639.1 molecular chaperone DnaJ -
  CB0101_RS08460 (CB0101_08445) grpE 1568252..1568932 (-) 681 WP_010312637.1 nucleotide exchange factor GrpE -
  CB0101_RS08465 (CB0101_08450) - 1569028..1570869 (+) 1842 WP_043718381.1 GspE/PulE family protein -
  CB0101_RS08470 (CB0101_08455) pilT 1570953..1572035 (+) 1083 WP_010312632.1 type IV pilus twitching motility protein PilT Machinery gene
  CB0101_RS08475 (CB0101_08460) - 1572050..1573342 (+) 1293 WP_010312630.1 type II secretion system F family protein -
  CB0101_RS08480 (CB0101_08465) - 1573335..1573742 (-) 408 WP_246833869.1 type II toxin-antitoxin system VapC family toxin -
  CB0101_RS08485 (CB0101_08470) - 1573748..1573990 (-) 243 WP_010312626.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  CB0101_RS08490 (CB0101_08475) - 1574015..1575340 (-) 1326 WP_043718378.1 NAD(P)/FAD-dependent oxidoreductase -
  CB0101_RS08495 (CB0101_08480) dusA 1575366..1576364 (+) 999 WP_029553198.1 tRNA dihydrouridine(20/20a) synthase DusA -
  CB0101_RS08500 (CB0101_08485) - 1576393..1576923 (-) 531 WP_010312618.1 RNA-binding protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39499.96 Da        Isoelectric Point: 6.2563

>NTDB_id=358242 CB0101_RS08470 WP_010312632.1 1570953..1572035(+) (pilT) [Synechococcus sp. CB0101]
MELQIEQLMEELVESGGSDLHLAAGQPPYGRFSGALRPMREEKLSEDQCNRLIFSMINNAQRKQLEQTWELDCAYGLKGV
SRFRVNVYRQRGSYAACLRALGSSIPSLSSLDLPPVVEETSRRPRGLILVTGPTGSGKTTTLAALLDHINRSRAEHILTI
EDPIEFTYRNEQSVIHQRQLGEDTRSFAAALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RMVDVFPAEQQTQIRVQLSGSLVGVFSQTLCKRLNPQPGQLGRVMAQEILINTPAIANLIREGKTAQLYSQIQTGGQQGM
QTLERALANLVVKGAVSRDEAMAKASKPEELGRLLDGQSG

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=358242 CB0101_RS08470 WP_010312632.1 1570953..1572035(+) (pilT) [Synechococcus sp. CB0101]
ATGGAGCTCCAGATCGAGCAGCTGATGGAGGAGCTGGTGGAGAGCGGCGGCAGCGATCTGCACCTGGCAGCGGGCCAGCC
TCCCTACGGCCGCTTCAGCGGCGCGCTGCGGCCGATGCGCGAGGAGAAGCTCAGCGAAGACCAGTGCAACCGGCTGATCT
TCTCGATGATCAACAACGCCCAGCGCAAACAGCTGGAGCAAACCTGGGAGCTCGACTGCGCCTACGGCCTCAAGGGCGTG
TCGCGCTTCCGCGTGAATGTGTACCGGCAGCGGGGCAGCTATGCCGCCTGCCTGCGGGCCCTGGGCAGTTCAATCCCCAG
CCTCTCCAGCCTGGATCTGCCGCCGGTGGTGGAGGAAACCAGCCGCCGGCCGCGGGGGCTGATCCTGGTGACTGGCCCCA
CTGGCTCCGGCAAAACCACCACCCTGGCGGCCCTGCTGGATCACATCAACCGCAGCCGCGCCGAGCACATCCTCACGATC
GAGGACCCGATCGAATTCACTTACCGCAACGAGCAGAGCGTGATCCACCAGCGCCAGCTGGGGGAAGACACCCGCAGCTT
TGCGGCGGCCCTGCGGGCGGCCCTACGCGAAGACCCCGACGTGATCCTGGTGGGGGAGATGCGCGACCTGGAAACGATTC
AGCTGGCGATCACCGCGGCGGAAACCGGTCACCTTGTGTTCGGCACCCTCCACACCAGCTCCGCCGCTCAGACGGTGGAT
CGGATGGTGGATGTGTTCCCCGCCGAGCAGCAGACCCAGATCCGCGTGCAGCTGAGCGGTTCGCTGGTGGGGGTGTTCTC
CCAGACCCTCTGCAAGCGGCTCAACCCGCAGCCTGGGCAGTTAGGGCGGGTGATGGCGCAGGAGATCCTGATCAACACGC
CGGCCATCGCCAACCTGATCCGCGAGGGCAAGACGGCGCAGCTCTATTCCCAGATCCAGACCGGCGGCCAGCAAGGCATG
CAGACCCTGGAGCGGGCCCTGGCCAACCTGGTGGTGAAGGGCGCCGTGAGCCGGGACGAGGCGATGGCGAAAGCCAGCAA
ACCCGAGGAACTGGGCCGCCTGCTCGACGGCCAGAGCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7ZUN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.722

96.944

0.511
  pilT Pseudomonas stutzeri DSM 10701

51.862

96.944

0.503
  pilT Acinetobacter baylyi ADP1

50.716

96.944

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.02

99.167

0.486
  pilT Acinetobacter nosocomialis M2

50.143

96.944

0.486
  pilT Acinetobacter baumannii D1279779

50.143

96.944

0.486
  pilT Acinetobacter baumannii strain A118

50.143

96.944

0.486
  pilT Legionella pneumophila strain Lp02

51.032

94.167

0.481
  pilT Legionella pneumophila strain ERS1305867

51.032

94.167

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.292

95

0.478
  pilT Vibrio cholerae strain A1552

50.292

95

0.478
  pilT Neisseria meningitidis 8013

48.424

96.944

0.469
  pilT Neisseria gonorrhoeae MS11

48.138

96.944

0.467
  pilU Vibrio cholerae strain A1552

37.43

99.444

0.372
  pilU Pseudomonas stutzeri DSM 10701

38.109

96.944

0.369
  pilU Acinetobacter baylyi ADP1

37.216

97.778

0.364

Multiple sequence alignment