Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E6A55_RS15825 Genome accession   NZ_CP039287
Coordinates   3357202..3358245 (-) Length   347 a.a.
NCBI ID   WP_010815050.1    Uniprot ID   R7X984
Organism   Cupriavidus necator H16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3352202..3363245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E6A55_RS15795 (E6A55_15785) - 3353194..3353571 (+) 378 WP_011615990.1 CidA/LrgA family protein -
  E6A55_RS15800 (E6A55_15790) - 3353568..3354293 (+) 726 WP_010815045.1 LrgB family protein -
  E6A55_RS15805 (E6A55_15795) - 3354379..3354750 (+) 372 WP_010815046.1 VOC family protein -
  E6A55_RS15810 (E6A55_15800) - 3354773..3355462 (+) 690 WP_010815047.1 helix-turn-helix transcriptional regulator -
  E6A55_RS15815 (E6A55_15805) - 3355469..3355963 (-) 495 WP_010815048.1 glutathione peroxidase -
  E6A55_RS15820 (E6A55_15810) pilU 3356036..3357175 (-) 1140 WP_010815049.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E6A55_RS15825 (E6A55_15815) pilT 3357202..3358245 (-) 1044 WP_010815050.1 type IV pilus twitching motility protein PilT Machinery gene
  E6A55_RS15830 (E6A55_15820) - 3358309..3359004 (+) 696 WP_010815051.1 YggS family pyridoxal phosphate-dependent enzyme -
  E6A55_RS15835 (E6A55_15825) proC 3359051..3359887 (+) 837 WP_011615991.1 pyrroline-5-carboxylate reductase -
  E6A55_RS15840 (E6A55_15830) ubiA 3359987..3360844 (-) 858 WP_010815053.1 4-hydroxybenzoate octaprenyltransferase -
  E6A55_RS15845 (E6A55_15835) - 3360986..3361471 (-) 486 WP_010815054.1 Dps family protein -
  E6A55_RS15850 (E6A55_15840) - 3361565..3361762 (-) 198 WP_041687510.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38381.18 Da        Isoelectric Point: 6.9772

>NTDB_id=357886 E6A55_RS15825 WP_010815050.1 3357202..3358245(-) (pilT) [Cupriavidus necator H16]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINIASMTHKDVHAMVYDIMSDTQRKAYEERLEIDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNESDMGHILTVED
PIEFVHSSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSVISYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=357886 E6A55_RS15825 WP_010815050.1 3357202..3358245(-) (pilT) [Cupriavidus necator H16]
ATGGACATCGCGCAGCTATTGGCTTTCGCTGTCAAGAACAAGGCGTCTGATCTTCATCTCTCCGCGGACATGCCGCCGAT
GGTGCGGATCCACGGCGACATGCGCCGGATCAACATCGCCTCGATGACGCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGGCCTACGAAGAACGCCTCGAAATCGATTTCTCGTTCGAGATCGCCGGCCTGTCCCGC
TTCCGGGTCAATGCCTATAACACCCAGCGCGGCGCCGCCGCGGTGTTCCGTACCATTCCCTCCAAGGTGCTCACGCTCGA
AGAGCTGCGCGCGCCCGCGGTGTTTGCCGACCTGTGCATGAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTCGACCATCGCAACGAAAGCGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACAGCTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGGCCGCACACGCATTCGTTTGCCAA
CGCGTTGCGCTCGGCACTGCGTGAAGACCCGGACGTGATCCTGGTGGGTGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTCTTCGGCACGCTGCACACCAGCTCAGCGGCCAAGACCATCGACCGCGTG
GTCGATGTGTTCCCGCCCGAAGAGAAGGACATGGTGCGCACCATGCTGTCGGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTGAAGACGCGCGACGGCAATGGCCGCACCGCCGCGCACGAGATCATGATCGCCACGCCCGCGATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGTCTGACCTGATCAAGCGCAGCGTCATCAGCTACAACGACGCGCGCGCCATCGCCAAGAACCCCGACGCGTTCATGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R7X984

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

71.676

99.712

0.715
  pilT Acinetobacter baumannii strain A118

71.676

99.712

0.715
  pilT Acinetobacter nosocomialis M2

71.676

99.712

0.715
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.424

0.695
  pilT Pseudomonas aeruginosa PAK

69.855

99.424

0.695
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Legionella pneumophila strain Lp02

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Neisseria meningitidis 8013

67.246

99.424

0.669
  pilT Legionella pneumophila strain ERS1305867

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.118

0.64
  pilT Vibrio cholerae strain A1552

65.875

97.118

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.424

0.493
  pilU Acinetobacter baylyi ADP1

40.116

99.135

0.398
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389

Multiple sequence alignment