Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E5790_RS07015 Genome accession   NZ_CP039255
Coordinates   1471765..1472892 (+) Length   375 a.a.
NCBI ID   WP_014645840.1    Uniprot ID   A0A397M567
Organism   Stenotrophomonas sp. PAMC25021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1466765..1477892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5790_RS06990 (E5790_06985) - 1466868..1468040 (-) 1173 WP_136191716.1 cystathionine gamma-synthase -
  E5790_RS06995 (E5790_06990) - 1468164..1469534 (-) 1371 WP_014645842.1 pyridoxal-phosphate dependent enzyme -
  E5790_RS07000 (E5790_06995) - 1469836..1470072 (-) 237 WP_005407964.1 YdcH family protein -
  E5790_RS07005 (E5790_07000) - 1470397..1471281 (-) 885 WP_080281838.1 OmpA family protein -
  E5790_RS07010 (E5790_07005) - 1471278..1471643 (-) 366 WP_005415160.1 DUF4398 domain-containing protein -
  E5790_RS07015 (E5790_07010) pilU 1471765..1472892 (+) 1128 WP_014645840.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5790_RS07020 (E5790_07015) - 1473024..1474307 (+) 1284 WP_111205550.1 putative DNA modification/repair radical SAM protein -
  E5790_RS07025 (E5790_07020) - 1474288..1475748 (+) 1461 WP_111205551.1 UdgX family uracil-DNA binding protein -
  E5790_RS07030 (E5790_07025) maiA 1475793..1476461 (-) 669 WP_032957926.1 maleylacetoacetate isomerase -
  E5790_RS07035 (E5790_07030) - 1476476..1477465 (-) 990 WP_111205552.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41396.73 Da        Isoelectric Point: 6.3741

>NTDB_id=357668 E5790_RS07015 WP_014645840.1 1471765..1472892(+) (pilU) [Stenotrophomonas sp. PAMC25021]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGIIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=357668 E5790_RS07015 WP_014645840.1 1471765..1472892(+) (pilU) [Stenotrophomonas sp. PAMC25021]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCCGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGGATGGTCATCCGCGCCATCCGCAGCCGCATCCCTAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCGGAAACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGGCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAATACGCCGATGATCCGCGACCTGTTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGGCTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCGGGCATCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCCCTGAA
GTTCCGTTTGTCCGAAGGCAGCAGCGGCGAGCACGACCCGTACGCCGATTTCTCGGCCGGCGCACCCAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A397M567

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.765

90.667

0.379
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363

Multiple sequence alignment