Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5677_RS11750 Genome accession   NZ_CP039251
Coordinates   2839033..2840088 (+) Length   351 a.a.
NCBI ID   WP_062843734.1    Uniprot ID   A0A2A2B977
Organism   Psychrobacter sp. PAMC27889     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2834033..2845088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5677_RS11735 (E5677_11735) - 2834668..2836350 (+) 1683 WP_179288328.1 NAD+ synthase -
  E5677_RS11740 (E5677_11740) - 2836590..2837864 (+) 1275 WP_062843736.1 hemolysin family protein -
  E5677_RS11745 (E5677_11745) - 2838035..2838757 (-) 723 WP_062843735.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5677_RS11750 (E5677_11750) pilT 2839033..2840088 (+) 1056 WP_062843734.1 type IV pilus twitching motility protein PilT Machinery gene
  E5677_RS11755 (E5677_11755) pilU 2840236..2841357 (+) 1122 WP_062843733.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5677_RS11760 (E5677_11760) fur 2841495..2841929 (-) 435 WP_062843732.1 ferric iron uptake transcriptional regulator -
  E5677_RS11765 (E5677_11765) - 2842254..2842673 (+) 420 WP_062845808.1 outer membrane protein assembly factor BamE -
  E5677_RS11770 (E5677_11770) - 2842722..2843105 (-) 384 WP_228139887.1 RnfH family protein -
  E5677_RS11775 (E5677_11775) - 2843093..2844214 (-) 1122 WP_062843731.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39046.92 Da        Isoelectric Point: 6.8441

>NTDB_id=357619 E5677_RS11750 WP_062843734.1 2839033..2840088(+) (pilT) [Psychrobacter sp. PAMC27889]
MAEKNPLSIEDLLRFTVKHKASDLHISAGMPAMIRADGEMTRINMPSMTHQVVHQLIYDIMNDKQRADFEEFFETDFSFE
IPNLARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFKRVSDFKRGVVLVTGPTGSGKSTTLAAMIDYINETRKEH
ILTIEDPIEFVHESKKSLINQREVHRDTLGFEPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAA
KTIDRVIDVFPAAEKDMIRAMLSESLQAVISQTLLRRQTGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGDGMI
TLDQTLKNLVGKGTISKDVARPYAKQPEAFL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=357619 E5677_RS11750 WP_062843734.1 2839033..2840088(+) (pilT) [Psychrobacter sp. PAMC27889]
ATGGCTGAAAAAAACCCCTTAAGTATTGAAGATTTGCTTCGCTTCACCGTCAAGCATAAAGCATCAGATTTACATATTTC
AGCTGGTATGCCGGCTATGATTCGCGCCGATGGTGAGATGACTAGAATCAATATGCCGTCAATGACGCATCAAGTGGTGC
ATCAGCTTATTTATGACATCATGAATGATAAGCAGCGTGCCGATTTTGAAGAGTTTTTTGAAACAGATTTTTCTTTTGAG
ATTCCCAATTTGGCCCGTTTTCGGGTAAACGCATTTAATCAAAATCGTGGTGCTGGCGCTGTTTTTCGGACCATTCCCTC
TAAAGTTCTTACCATGGAAGATTTGGGTATGGGCGATGTATTTAAACGTGTCTCAGATTTTAAGCGTGGTGTCGTATTGG
TGACAGGCCCTACAGGTTCAGGCAAATCAACCACATTGGCAGCCATGATTGACTATATCAATGAAACTCGTAAAGAGCAT
ATTTTAACCATTGAAGACCCCATTGAATTTGTCCATGAATCCAAAAAAAGTCTTATCAACCAACGTGAGGTTCACCGCGA
TACATTAGGCTTCGAGCCAGCACTTCGATCTGCCCTACGTGAGGATCCAGATGTTATTTTGGTCGGTGAGCTACGTGACC
TTGAAACCATTCGTTTGGCATTGACGGCCGCAGAAACTGGTCACTTAGTATTCGGCACACTACACACAAGCTCAGCCGCC
AAGACGATTGACCGAGTTATCGATGTATTTCCTGCAGCAGAAAAAGACATGATTCGTGCCATGTTATCTGAATCATTACA
AGCGGTGATTTCACAAACGCTGCTACGTCGTCAAACAGGCGGGCGTGTTGCTGCACACGAAATCATGCTGGGCACACCTG
CTATTCGTAACTTGATACGTGAAAATAAAATCGCTCAGATGTATTCAGCGATTCAGACTGGGGCAGGTGACGGCATGATT
ACCCTTGATCAAACCTTGAAGAACTTAGTAGGAAAAGGCACCATCAGTAAAGATGTGGCTCGCCCTTATGCCAAACAGCC
TGAGGCATTTTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A2B977

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

79.13

98.291

0.778
  pilT Acinetobacter nosocomialis M2

79.13

98.291

0.778
  pilT Acinetobacter baumannii D1279779

79.13

98.291

0.778
  pilT Pseudomonas aeruginosa PAK

77.907

98.006

0.764
  pilT Pseudomonas stutzeri DSM 10701

77.326

98.006

0.758
  pilT Acinetobacter baylyi ADP1

76.163

98.006

0.746
  pilT Legionella pneumophila strain Lp02

71.512

98.006

0.701
  pilT Legionella pneumophila strain ERS1305867

71.512

98.006

0.701
  pilT Neisseria meningitidis 8013

64.348

98.291

0.632
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.477

93.732

0.632
  pilT Vibrio cholerae strain A1552

67.477

93.732

0.632
  pilT Neisseria gonorrhoeae MS11

63.768

98.291

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

98.291

0.501
  pilU Acinetobacter baylyi ADP1

41.691

97.721

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.708

96.581

0.393
  pilU Vibrio cholerae strain A1552

42.415

92.023

0.39

Multiple sequence alignment