Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5284_RS03800 Genome accession   NZ_CP038856
Coordinates   769047..770027 (+) Length   326 a.a.
NCBI ID   WP_135911434.1    Uniprot ID   A0A4U6K691
Organism   Citrobacter freundii strain Upstream_1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 764047..775027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5284_RS03770 (E5284_03785) ansB 764552..765598 (+) 1047 WP_096755739.1 L-asparaginase 2 -
  E5284_RS03775 (E5284_03790) hemW 765723..766859 (-) 1137 WP_135911432.1 radical SAM family heme chaperone HemW -
  E5284_RS03780 (E5284_03795) - 766852..767445 (-) 594 WP_135911433.1 XTP/dITP diphosphatase -
  E5284_RS03785 (E5284_03800) yggU 767453..767743 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  E5284_RS03790 (E5284_03805) - 767740..768306 (-) 567 WP_096755742.1 YggT family protein -
  E5284_RS03795 (E5284_03810) - 768325..769029 (-) 705 WP_096755743.1 YggS family pyridoxal phosphate-dependent enzyme -
  E5284_RS03800 (E5284_03815) pilT 769047..770027 (+) 981 WP_135911434.1 type IV pilus twitching motility protein PilT Machinery gene
  E5284_RS03805 (E5284_03820) ruvX 770037..770453 (-) 417 WP_096755745.1 Holliday junction resolvase RuvX -
  E5284_RS03810 (E5284_03825) - 770453..771016 (-) 564 WP_096755746.1 YqgE/AlgH family protein -
  E5284_RS03815 (E5284_03830) gshB 771192..772139 (-) 948 WP_135911435.1 glutathione synthase -
  E5284_RS03820 (E5284_03835) rsmE 772152..772883 (-) 732 WP_135911436.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E5284_RS03825 (E5284_03840) endA 772958..773665 (-) 708 WP_117342640.1 deoxyribonuclease I -
  E5284_RS03830 (E5284_03845) - 773903..774778 (+) 876 WP_135911437.1 dihydrodipicolinate synthase family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35813.03 Da        Isoelectric Point: 6.4952

>NTDB_id=355905 E5284_RS03800 WP_135911434.1 769047..770027(+) (pilT) [Citrobacter freundii strain Upstream_1]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRVEIAPFTAPDVEKLLMLWLSEQQQVQWREKGQVDFAIALADSRRLRA
SAFTHQQGTSLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVDSLNQHVDGHILTLEDPIE
FRYTSQRCLIQQREIGVHCLSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEADRQGGRVALFELLVNTSAVSNLIREGKTHQLPGVIQTGQQVGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=355905 E5284_RS03800 WP_135911434.1 769047..770027(+) (pilT) [Citrobacter freundii strain Upstream_1]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGGTAGAAATTGCCCCGTTTACCGCGCCTGACGTGGAGAAGCTGCTGATGTTATGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAAGGGTCAGGTTGATTTTGCTATCGCACTGGCGGATTCCCGGCGTTTACGGGCC
AGCGCATTTACGCATCAGCAGGGAACGTCACTGGCGCTGAGACTGCTGCCGCTTGATTGCCCGTGCCTGGATGATCTCCA
GACGCCCGCTGCGTTGCCAGAACTGCTGCACAGCGAAAACGGGTTGATTCTGGTGACCGGGGCGACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTCTCTCAATCAGCACGTTGACGGGCATATTCTGACCCTGGAAGACCCGATTGAA
TTCCGCTATACCAGCCAGCGTTGTCTGATCCAACAGCGTGAGATTGGCGTGCACTGCCTTTCTTTTGCTGCCGGCCTGCG
GGCGGCATTGCGTGAAGATCCTGACGTGATTTTACTGGGGGAACTGCGCGACAGCGAGACGATTCGTCTGGCACTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGTGCGGCGCAGGCGGTGGAGCGGCTGGTGGATACG
TTCCCTGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGTAGTTTGCGGGCGGTGCTCGCACAAAAACTGGAAGC
CGACAGGCAGGGTGGGCGGGTGGCGTTATTTGAACTGCTGGTCAATACGTCGGCTGTGAGTAATCTGATCCGTGAAGGGA
AAACCCACCAGCTACCCGGCGTGATACAAACCGGGCAACAGGTGGGTATGCAAACATTTGCCCAGAGCCTGCAGCAGCGT
CAGGCGCAAGGCAGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U6K691

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Legionella pneumophila strain ERS1305867

46.894

98.773

0.463
  pilT Legionella pneumophila strain Lp02

46.894

98.773

0.463
  pilT Acinetobacter baylyi ADP1

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.711

97.546

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

37.654

99.387

0.374

Multiple sequence alignment