Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E4174_RS21120 Genome accession   NZ_CP038653
Coordinates   4297569..4298549 (+) Length   326 a.a.
NCBI ID   WP_048225499.1    Uniprot ID   -
Organism   Citrobacter freundii strain 154     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4292569..4303549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4174_RS21080 (E4174_21675) hemW 4292795..4293931 (-) 1137 WP_048225503.1 radical SAM family heme chaperone HemW -
  E4174_RS21085 (E4174_21680) - 4293924..4294517 (-) 594 WP_003825414.1 XTP/dITP diphosphatase -
  E4174_RS21090 (E4174_21685) yggU 4294525..4294815 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  E4174_RS21095 (E4174_21690) - 4294812..4295378 (-) 567 WP_003825417.1 YggT family protein -
  E4174_RS21100 (E4174_21695) - 4295397..4296101 (-) 705 WP_048215572.1 YggS family pyridoxal phosphate-dependent enzyme -
  E4174_RS21105 (E4174_21700) - 4296119..4296244 (+) 126 Protein_4162 type IV pili twitching motility protein PilT -
  E4174_RS21110 (E4174_21710) - 4296956..4297375 (+) 420 WP_048225501.1 DUF3828 domain-containing protein -
  E4174_RS21115 (E4174_21715) - 4297381..4297551 (-) 171 Protein_4164 YggS family pyridoxal phosphate enzyme -
  E4174_RS21120 (E4174_21720) pilT 4297569..4298549 (+) 981 WP_048225499.1 type IV pilus twitching motility protein PilT Machinery gene
  E4174_RS21125 (E4174_21725) ruvX 4298546..4298962 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  E4174_RS21130 (E4174_21730) - 4298962..4299525 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  E4174_RS21135 (E4174_21735) gshB 4299702..4300649 (-) 948 WP_003027083.1 glutathione synthase -
  E4174_RS21140 (E4174_21740) rsmE 4300669..4301400 (-) 732 WP_008785768.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E4174_RS21145 (E4174_21745) endA 4301475..4302182 (-) 708 WP_153983669.1 deoxyribonuclease I -
  E4174_RS21150 (E4174_21750) - 4302277..4302774 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35915.01 Da        Isoelectric Point: 6.0939

>NTDB_id=355347 E4174_RS21120 WP_048225499.1 4297569..4298549(+) (pilT) [Citrobacter freundii strain 154]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLSEQQQVQWQEQGQIDFAIALAGSQRLRA
SAFVHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQQVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FSATEKEPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=355347 E4174_RS21120 WP_048225499.1 4297569..4298549(+) (pilT) [Citrobacter freundii strain 154]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCAGGAACAGGGGCAGATTGATTTTGCTATTGCGCTGGCAGGTTCCCAGCGGCTGCGCGCC
AGTGCTTTTGTTCATCAACAGGGAACCTCACTGGCGCTAAGATTGCTCCCGCTTGATTGTCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTTGAGTACCTTAATCAGCAAGTTGCTGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTGCACTGCGCTTCTTTTGCCGCTGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAGCTACGCGACGTGGAAACCATTCGTCTGGCGTTAACGG
CAGCGGAAACTGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTTCGGCAACAGAGAAAGAGCCGGTGCGTAACCAACTGGCAGATAGCCTGAGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAACAGGGAGGGCGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGTCAGCAGCAGCGT
CAGGCGCAAGGACGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.667

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter baumannii D1279779

44.985

100

0.454
  pilT Acinetobacter baumannii strain A118

44.985

100

0.454
  pilT Acinetobacter baylyi ADP1

44.985

100

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Acinetobacter nosocomialis M2

44.681

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368

Multiple sequence alignment