Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E2566_RS17195 Genome accession   NZ_CP038498
Coordinates   3791058..3792074 (-) Length   338 a.a.
NCBI ID   WP_107169994.1    Uniprot ID   -
Organism   Pectobacterium punjabense strain SS95     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3786058..3797074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2566_RS17180 (E2566_17330) nsrR 3786460..3786885 (-) 426 WP_103160677.1 nitric oxide-sensing transcriptional repressor NsrR -
  E2566_RS17185 (E2566_17335) - 3787174..3788745 (+) 1572 WP_107169996.1 methyl-accepting chemotaxis protein -
  E2566_RS17190 (E2566_17340) - 3789257..3791089 (+) 1833 WP_107169995.1 sensor domain-containing diguanylate cyclase -
  E2566_RS17195 (E2566_17345) pilT 3791058..3792074 (-) 1017 WP_107169994.1 type IV pilus twitching motility protein PilT Machinery gene
  E2566_RS17200 (E2566_17350) - 3792096..3792809 (+) 714 WP_107169993.1 YggS family pyridoxal phosphate-dependent enzyme -
  E2566_RS17205 (E2566_17355) proC 3792896..3793717 (+) 822 WP_107170052.1 pyrroline-5-carboxylate reductase -
  E2566_RS17210 (E2566_17360) - 3793767..3794321 (+) 555 WP_107169992.1 YggT family protein -
  E2566_RS17215 (E2566_17365) yggU 3794318..3794608 (+) 291 WP_052902894.1 DUF167 family protein YggU -
  E2566_RS17220 (E2566_17370) - 3794626..3795219 (+) 594 WP_107169991.1 XTP/dITP diphosphatase -
  E2566_RS17225 (E2566_17375) hemW 3795212..3796351 (+) 1140 WP_107169990.1 radical SAM family heme chaperone HemW -
  E2566_RS17230 (E2566_17380) - 3796583..3796732 (+) 150 Protein_3366 ribbon-helix-helix domain-containing protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36824.20 Da        Isoelectric Point: 6.4225

>NTDB_id=354816 E2566_RS17195 WP_107169994.1 3791058..3792074(-) (pilT) [Pectobacterium punjabense strain SS95]
MELDEWMARSVKHNASDLHLCSGHPPVLRMDGRLQPENTLPCLAPEQVAQWCDAWLEPTQKEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSVALRIIPFVQPSLDELHAPPVLATLLGKPNGLILITGATGSGKSTTLAAMVGSLNDSSDRHVITLE
DPIEFIHTSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVAQKLLPAVQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGTAVGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=354816 E2566_RS17195 WP_107169994.1 3791058..3792074(-) (pilT) [Pectobacterium punjabense strain SS95]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGGCATCCTCCGGT
GTTACGTATGGATGGGCGGCTACAGCCTGAAAATACCTTACCATGTTTAGCGCCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGACTCAGAAAGAGCAATTGCGCCAGGCAGGGCAGGTGGATGGGGCGTTAATGTTGCCTGATGGGCAG
CGCCTGCGGGTCAATGTGTTTCGCCAGCGGGAAGGGTTGTCTGTCGCGCTGCGTATTATCCCGTTTGTTCAACCTTCGTT
GGATGAATTACATGCGCCACCCGTCCTCGCGACACTGCTGGGGAAGCCAAACGGGCTGATTCTGATCACTGGTGCGACGG
GCAGCGGTAAATCCACGACGCTGGCGGCAATGGTTGGGAGCTTAAACGACAGCAGCGATCGCCATGTGATCACGCTGGAA
GATCCGATCGAGTTTATCCATACCAGCCGACGATGCCTGATCCAACAGCGAGAGATCGGTGCACACAGCGCATCTTTTGC
GCAGGCGCTACGAGCGGCATTGCGGGAAGATCCCGATGTTATTCTGCTAGGGGAACTGCGCGATACGGAGACGATTCGTC
TGGCGTTGACGGCGGCGGAAACCGGCCATTTGGTGCTATCAACGCTGCATACACGCAGTGCATCGCAGGCCGTTGATCGG
CTAATTGATGTCTTCCCCGGTGAAGAGAAAGCGTATGTTCGCAGCCAGTTAGCAACCTGTTTGCAAGCCGTAGTGGCGCA
AAAGTTGTTGCCCGCAGTGCAAGGTGGCCGAATCGCCCTTTATGAAGTGCTGACGGCGACAGCGGCAGTCAGCAACTTGA
TTCGTGAGGGGAAAACGCACCAGCTTCCCGGCTTGATCCAGACGGGAACGGCAGTGGGAATGCAAACGTTCGAACAAAGC
TATCAGCAGCGCTGTCGGGACGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.909

97.633

0.497
  pilT Vibrio cholerae strain A1552

50.909

97.633

0.497
  pilT Neisseria meningitidis 8013

49.398

98.225

0.485
  pilT Neisseria gonorrhoeae MS11

49.096

98.225

0.482
  pilT Legionella pneumophila strain ERS1305867

48.036

97.929

0.47
  pilT Legionella pneumophila strain Lp02

48.036

97.929

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.036

97.929

0.47
  pilT Pseudomonas aeruginosa PAK

47.734

97.929

0.467
  pilT Acinetobacter baylyi ADP1

47.432

97.929

0.464
  pilT Acinetobacter nosocomialis M2

46.828

97.929

0.459
  pilT Acinetobacter baumannii D1279779

46.828

97.929

0.459
  pilT Acinetobacter baumannii strain A118

46.828

97.929

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.543

99.704

0.414
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

40.24

98.521

0.396
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367

Multiple sequence alignment