Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E4Z61_RS20820 Genome accession   NZ_CP038469
Coordinates   4205352..4206332 (+) Length   326 a.a.
NCBI ID   WP_135324368.1    Uniprot ID   -
Organism   Citrobacter tructae strain SNU WT2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4200352..4211332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4Z61_RS20790 (E4Z61_20795) - 4201402..4201961 (+) 560 Protein_3911 DUF1202 family protein -
  E4Z61_RS20795 (E4Z61_20800) hemW 4202028..4203164 (-) 1137 WP_135324365.1 radical SAM family heme chaperone HemW -
  E4Z61_RS20800 (E4Z61_20805) - 4203157..4203750 (-) 594 WP_135324366.1 XTP/dITP diphosphatase -
  E4Z61_RS20805 (E4Z61_20810) yggU 4203758..4204048 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  E4Z61_RS20810 (E4Z61_20815) - 4204045..4204611 (-) 567 WP_003825417.1 YggT family protein -
  E4Z61_RS20815 (E4Z61_20820) - 4204630..4205334 (-) 705 WP_135324367.1 YggS family pyridoxal phosphate-dependent enzyme -
  E4Z61_RS20820 (E4Z61_20825) pilT 4205352..4206332 (+) 981 WP_135324368.1 type IV pilus twitching motility protein PilT Machinery gene
  E4Z61_RS20825 (E4Z61_20830) ruvX 4206329..4206745 (-) 417 WP_135324369.1 Holliday junction resolvase RuvX -
  E4Z61_RS20830 (E4Z61_20835) - 4206745..4207308 (-) 564 WP_205746873.1 YqgE/AlgH family protein -
  E4Z61_RS20835 (E4Z61_20840) gshB 4207420..4208367 (-) 948 WP_135324371.1 glutathione synthase -
  E4Z61_RS20840 (E4Z61_20845) rsmE 4208525..4209256 (-) 732 WP_135324372.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E4Z61_RS20845 (E4Z61_20850) endA 4209332..4210039 (-) 708 WP_135324373.1 deoxyribonuclease I -
  E4Z61_RS20850 (E4Z61_20855) - 4210134..4210631 (-) 498 WP_135324374.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35786.90 Da        Isoelectric Point: 6.1714

>NTDB_id=354692 E4Z61_RS20820 WP_135324368.1 4205352..4206332(+) (pilT) [Citrobacter tructae strain SNU WT2]
MNMEEIVALSVKHNVSDLHLCNAWPARWRICGRMETAPFTAPDVDALLLSWLSEQQQVQWRENGQVDFAIALADSRRLRA
SAFAHQQGTSLALRLLPVACPQLDELQTPAVLPELLHSENGLILVTGATGSGKSTTLAAMVDYLNQHVEGHILTLEDPIE
YRYTSQRCLIQQREIGVHCTSFAAGLRAALREDPDVILLGELRDGETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLEEDKQGGRVALFEMLVNIPAVGNLIREGKTHQLPGVIQTGQQVGMQTFAQSQQQR
QALGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=354692 E4Z61_RS20820 WP_135324368.1 4205352..4206332(+) (pilT) [Citrobacter tructae strain SNU WT2]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATATGTGGAAGGATGGAAACCGCACCGTTTACCGCGCCTGACGTGGATGCGCTGCTATTGAGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAAAATGGCCAGGTTGATTTTGCCATCGCGTTGGCAGATTCCCGGCGTTTGCGGGCT
AGCGCATTTGCCCATCAGCAGGGAACTTCGCTGGCGCTGCGACTGTTGCCAGTTGCTTGCCCGCAGTTAGATGAGCTCCA
GACGCCCGCTGTTTTGCCAGAACTGCTGCACAGTGAAAACGGATTGATTCTGGTGACAGGGGCAACGGGCAGCGGTAAGT
CGACGACGCTGGCGGCGATGGTGGACTATCTTAATCAACACGTTGAAGGACATATTCTGACTCTGGAAGACCCGATTGAA
TACCGCTATACCAGTCAGCGTTGTTTGATCCAACAGCGTGAGATTGGTGTGCACTGCACCTCTTTTGCTGCCGGTCTTCG
AGCCGCATTACGTGAAGATCCGGATGTGATTTTGCTGGGGGAGCTGCGCGATGGCGAGACCATTCGTCTGGCGCTCACCG
CAGCGGAAACCGGGCATTTGGTGCTGGCAACGTTGCATACACGCGGTGCGGCGCAGGCGGTGGAGCGGCTAGTGGATACG
TTTCCCGCGCAGGAAAAAGATCCGGTGCGTAATCAACTGGCGGGTAGCTTGCGGGCGGTGCTTGCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGGCGCGTGGCGTTATTCGAAATGCTCGTCAACATACCTGCCGTCGGCAATCTGATCCGTGAAGGGA
AAACTCACCAGTTACCCGGTGTGATACAAACCGGGCAACAGGTGGGCATGCAGACGTTTGCACAGAGCCAACAGCAGCGG
CAGGCGCTGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.72

100

0.482
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.203

96.933

0.448
  pilT Pseudomonas aeruginosa PAK

46.203

96.933

0.448
  pilT Legionella pneumophila strain Lp02

45.912

97.546

0.448
  pilT Legionella pneumophila strain ERS1305867

45.912

97.546

0.448
  pilT Acinetobacter baumannii D1279779

45.768

97.853

0.448
  pilT Acinetobacter nosocomialis M2

45.768

97.853

0.448
  pilT Acinetobacter baumannii strain A118

45.768

97.853

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.902

97.239

0.417
  pilU Vibrio cholerae strain A1552

40.683

98.773

0.402
  pilU Pseudomonas stutzeri DSM 10701

38.75

98.16

0.38
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368

Multiple sequence alignment