Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E4T63_RS26435 Genome accession   NZ_CP038438
Coordinates   5708729..5709763 (-) Length   344 a.a.
NCBI ID   WP_003229114.1    Uniprot ID   A0A031GC41
Organism   Pseudomonas fluorescens strain LBUM677     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5703729..5714763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T63_RS26400 (E4T63_26400) ruvX 5703811..5704248 (+) 438 WP_003229101.1 Holliday junction resolvase RuvX -
  E4T63_RS26405 (E4T63_26405) pyrR 5704330..5704836 (+) 507 WP_027610583.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  E4T63_RS26410 (E4T63_26410) - 5704865..5705869 (+) 1005 WP_003229107.1 aspartate carbamoyltransferase catalytic subunit -
  E4T63_RS26415 (E4T63_26415) - 5705866..5707137 (+) 1272 WP_098966064.1 dihydroorotase -
  E4T63_RS26420 (E4T63_26420) - 5707290..5707721 (-) 432 WP_007964113.1 TM2 domain-containing protein -
  E4T63_RS26425 (E4T63_26425) - 5707970..5708596 (+) 627 WP_003229112.1 C40 family peptidase -
  E4T63_RS26435 (E4T63_26435) pilT 5708729..5709763 (-) 1035 WP_003229114.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E4T63_RS26440 (E4T63_26440) - 5709820..5710506 (+) 687 WP_098966066.1 YggS family pyridoxal phosphate-dependent enzyme -
  E4T63_RS26445 (E4T63_26445) proC 5710535..5711353 (+) 819 WP_135296758.1 pyrroline-5-carboxylate reductase -
  E4T63_RS26450 (E4T63_26450) - 5711364..5711954 (+) 591 WP_041063533.1 YggT family protein -
  E4T63_RS26455 (E4T63_26455) - 5711951..5712247 (+) 297 WP_135296759.1 DUF167 domain-containing protein -
  E4T63_RS26460 (E4T63_26460) - 5712476..5713615 (+) 1140 WP_135296760.1 homoserine O-acetyltransferase -
  E4T63_RS26465 (E4T63_26465) metW 5713623..5714243 (+) 621 WP_003229126.1 methionine biosynthesis protein MetW -
  E4T63_RS26470 (E4T63_26470) - 5714270..5714704 (+) 435 WP_135296761.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38071.74 Da        Isoelectric Point: 7.0158

>NTDB_id=354298 E4T63_RS26435 WP_003229114.1 5708729..5709763(-) (pilT) [Pseudomonas fluorescens strain LBUM677]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KELVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=354298 E4T63_RS26435 WP_003229114.1 5708729..5709763(-) (pilT) [Pseudomonas fluorescens strain LBUM677]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTCGATGGCGATGTGCGGCGGATCAATCTGCCGGCGCTGGATCACAAGCAAGTGCACGAATTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAGAAACACCTCGAAACGGATTTCTCGTTCGAAGTCCCCGGCGTGGCGCGC
TTTCGGGTCAACGCGTTCAACCAGAACCGGGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCGAAAGTGCTGAGCATGGA
AGACCTCGGCATGGGCGATGTCTTTCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGGT
CCGGCAAATCCACCACGCTGGCGGCGATGATCGATTACCTCAATACCCATCGCCATCACCATATCCTCACCATCGAAGAC
CCGATCGAATTCGTCCACGAATCGCGCAAGTGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGCAGCTTTGCCAC
GGCGCTGCGCTCGGCACTGCGCGAAGACCCGGACGTGATTCTGGTTGGCGAGATGCGCGACCTGGAAACCATTCGCCTCG
CACTGACTGCCGCTGAAACCGGGCATCTGGTGTTCGGCACGCTGCACACGACGTCGGCCGCCAAGACCATCGACCGTGTT
GTCGATGTGTTCCCGGGCGACGAGAAGTCCATGGTGCGTTCGATGCTGTCCGAGTCATTGCTGGCGGTGGTGTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGGCGGGTGGCAGCCCACGAGATCATGCTGGGAACATCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAAATGTATTCGGCGATTCAGACCGGCGGGTCGTTGGGGATGCAGACACTCGACATGTGCCTG
AAGGAGCTGGTGACCAAGGGCTTGATCAGCCGCGAGCATGCGCGGGAAAAGGCGCGTACGCCGGATAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A031GC41

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

73.077

98.256

0.718
  pilT Legionella pneumophila strain ERS1305867

73.077

98.256

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398

Multiple sequence alignment