Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DTOX_RS15780 Genome accession   NC_013216
Coordinates   3453856..3454938 (-) Length   360 a.a.
NCBI ID   WP_015758683.1    Uniprot ID   C8W5J1
Organism   Desulfofarcimen acetoxidans DSM 771     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3448856..3459938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTOX_RS15750 (Dtox_3250) - 3448940..3449530 (-) 591 WP_015758677.1 hypothetical protein -
  DTOX_RS15755 (Dtox_3251) pilO 3449560..3450093 (-) 534 WP_015758678.1 type 4a pilus biogenesis protein PilO -
  DTOX_RS15760 (Dtox_3252) - 3450083..3450805 (-) 723 WP_015758679.1 PilN domain-containing protein -
  DTOX_RS15765 (Dtox_3253) pilM 3450807..3451859 (-) 1053 WP_015758680.1 type IV pilus assembly protein PilM -
  DTOX_RS15770 (Dtox_3254) - 3451879..3452598 (-) 720 WP_015758681.1 sigma-70 family RNA polymerase sigma factor -
  DTOX_RS15775 (Dtox_3255) - 3452604..3453902 (-) 1299 WP_340139997.1 type II secretion system F family protein -
  DTOX_RS15780 (Dtox_3256) pilT 3453856..3454938 (-) 1083 WP_015758683.1 type IV pilus twitching motility protein PilT Machinery gene
  DTOX_RS15785 (Dtox_3257) - 3454938..3456623 (-) 1686 WP_015758684.1 ATPase, T2SS/T4P/T4SS family -
  DTOX_RS15790 (Dtox_3258) - 3456936..3457274 (-) 339 WP_015758685.1 phage holin family protein -
  DTOX_RS15795 (Dtox_3259) rpsT 3457402..3457668 (+) 267 WP_015758686.1 30S ribosomal protein S20 -
  DTOX_RS15800 (Dtox_3261) - 3458315..3459823 (-) 1509 WP_015758687.1 Fic family protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40420.72 Da        Isoelectric Point: 6.8927

>NTDB_id=35287 DTOX_RS15780 WP_015758683.1 3453856..3454938(-) (pilT) [Desulfofarcimen acetoxidans DSM 771]
MDEQKAVDVNEILKKMVQMDGSDLHLTAGMPPVVRVYGELLRMDYPKLMPRDIEDLVYPVLLPGQREQLEKEWELDFSYS
VPGVSRFRGNIMWQRGSLAVNFRAVAINIPDMKDLNLPPAVRGLSQLPRGLVLVTGPTGSGKSTTLASIIKLINEEQSLN
IVTIENPIEFLHSHKKSIIKQREVGTDTFSFASALRHVLRHDPDVILVGEMRDMESISIALTAAETGHLVYSTLHTQTAP
LALHRIIDVFPDSARNQIRQQLADSLQAVLAQQLIHRADGKGRVVAVELLLSTPAVRNLIREGKEHQLYTVMQTSRNTGM
QTMDQALAELFLSGKITRDMALEHCVDRVELERVLRRGQY

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=35287 DTOX_RS15780 WP_015758683.1 3453856..3454938(-) (pilT) [Desulfofarcimen acetoxidans DSM 771]
ATGGATGAGCAAAAAGCGGTTGATGTGAATGAGATATTAAAAAAAATGGTGCAAATGGACGGTTCAGACCTGCACCTGAC
AGCCGGTATGCCTCCGGTGGTGCGGGTCTACGGTGAGCTTTTGCGTATGGATTATCCCAAGCTGATGCCAAGGGATATAG
AGGATCTGGTTTATCCGGTTTTGCTGCCCGGTCAAAGGGAACAGCTGGAAAAAGAATGGGAATTGGATTTCTCTTATTCG
GTGCCGGGTGTCAGCCGTTTTCGGGGAAATATTATGTGGCAGAGAGGTTCCCTGGCGGTTAATTTCCGGGCGGTAGCCAT
AAATATTCCTGATATGAAGGACCTGAACCTGCCTCCGGCAGTCAGGGGTTTATCCCAGCTGCCCAGAGGACTGGTGCTGG
TTACCGGGCCTACGGGAAGCGGCAAGTCAACAACCCTGGCTTCCATTATTAAACTGATTAACGAAGAGCAGTCCTTAAAT
ATTGTTACTATCGAAAATCCCATAGAATTTTTGCACAGCCACAAAAAATCTATTATCAAGCAGAGAGAAGTGGGGACAGA
CACTTTTTCCTTTGCCAGTGCTCTGAGGCATGTACTGCGGCATGATCCGGATGTGATACTGGTGGGAGAGATGAGAGATA
TGGAGAGTATATCCATTGCCCTTACCGCCGCCGAGACAGGGCACCTGGTATATTCCACCCTGCATACGCAGACGGCCCCT
TTGGCCCTGCACCGTATTATTGATGTTTTTCCCGACAGCGCCCGCAATCAGATTCGTCAGCAGCTGGCCGATTCTCTGCA
GGCTGTTTTGGCCCAGCAGTTAATCCACCGGGCCGACGGCAAGGGAAGGGTGGTTGCGGTAGAGTTATTGCTGAGCACGC
CGGCTGTGAGAAACCTGATCAGAGAGGGCAAAGAGCACCAGCTTTATACGGTTATGCAGACTTCACGCAACACAGGCATG
CAGACCATGGATCAGGCTCTGGCTGAACTTTTCTTAAGCGGAAAAATTACGCGGGATATGGCCCTGGAGCACTGTGTTGA
CCGGGTTGAGCTGGAGCGTGTGCTGCGGCGGGGCCAGTATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C8W5J1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

96.944

0.486
  pilT Acinetobacter baumannii strain A118

50.602

92.222

0.467
  pilT Acinetobacter nosocomialis M2

50.602

92.222

0.467
  pilT Acinetobacter baumannii D1279779

50.602

92.222

0.467
  pilT Legionella pneumophila strain Lp02

48.397

95.278

0.461
  pilT Legionella pneumophila strain ERS1305867

48.397

95.278

0.461
  pilT Pseudomonas stutzeri DSM 10701

48.521

93.889

0.456
  pilT Pseudomonas aeruginosa PAK

48.521

93.889

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.547

91.944

0.456
  pilT Vibrio cholerae strain A1552

49.547

91.944

0.456
  pilT Acinetobacter baylyi ADP1

49.547

91.944

0.456
  pilT Neisseria meningitidis 8013

47.917

93.333

0.447
  pilT Neisseria gonorrhoeae MS11

47.904

92.778

0.444
  pilU Pseudomonas stutzeri DSM 10701

42.045

97.778

0.411
  pilU Vibrio cholerae strain A1552

40.469

94.722

0.383
  pilU Acinetobacter baylyi ADP1

40.588

94.444

0.383

Multiple sequence alignment