Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACI_RS01670 Genome accession   NC_013205
Coordinates   355636..356679 (-) Length   347 a.a.
NCBI ID   WP_012809743.1    Uniprot ID   -
Organism   Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 350636..361679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACI_RS01640 (Aaci_0304) - 350733..351623 (+) 891 WP_280959724.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AACI_RS01645 (Aaci_0305) - 351671..352420 (+) 750 WP_280959718.1 type II secretion system protein J -
  AACI_RS01650 (Aaci_0306) - 352531..353325 (+) 795 WP_245530660.1 hypothetical protein -
  AACI_RS01655 (Aaci_0307) pilM 353377..354345 (+) 969 WP_012809740.1 type IV pilus biogenesis protein PilM -
  AACI_RS01660 (Aaci_0308) - 354349..354954 (+) 606 WP_012809741.1 PilN domain-containing protein -
  AACI_RS01665 (Aaci_0309) gspM 354951..355535 (+) 585 WP_012809742.1 type II secretion system protein GspM -
  AACI_RS01670 (Aaci_0310) pilT 355636..356679 (-) 1044 WP_012809743.1 type IV pilus twitching motility protein PilT Machinery gene
  AACI_RS01675 (Aaci_0311) - 356824..357609 (-) 786 WP_012809744.1 A24 family peptidase -
  AACI_RS01680 (Aaci_0312) - 357788..360433 (+) 2646 WP_148213716.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38373.14 Da        Isoelectric Point: 7.2536

>NTDB_id=35251 AACI_RS01670 WP_012809743.1 355636..356679(-) (pilT) [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
MVQIDEILALAKEKEASDVHLSAGAPVTYRVHGDLEPMGSKLMPVDLEAFARQMLSEERWQRFMEAGEIDFSYSIPGVAR
FRVNAYRQRGSISIAARLIPQRIPTMEELNLPSVFKALMNRPYGLVLVTGPTGSGKSTTLAAVLDAINQTQRRHIITLED
PIEYLHTHKLSIIDQREIGQDTSGFAPALRAALRQDPDILLVGEMRDLETIATAITASETGHLVFATLHTSDAVQTVERI
IDVFPPEQQGQIRIQLAGVLAGVVSQRLHRTRDGRGRVASFEVLVNTPAVANLIRSGKTHQIGAMMQTGRQFGMITMEMS
IRELVQQGKISPAVLRQWQPAQLSQQS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=35251 AACI_RS01670 WP_012809743.1 355636..356679(-) (pilT) [Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446]
ATGGTGCAGATTGATGAAATCTTGGCTTTGGCAAAAGAGAAAGAAGCTTCAGACGTCCATCTCTCAGCCGGGGCGCCTGT
GACCTATCGCGTCCATGGCGATCTCGAACCCATGGGTTCCAAACTCATGCCGGTCGATCTCGAGGCCTTCGCGCGGCAGA
TGCTTTCGGAGGAGCGCTGGCAGCGGTTCATGGAGGCGGGCGAGATCGACTTTTCGTACAGCATTCCAGGCGTGGCTCGC
TTCCGGGTCAATGCGTATCGGCAGCGGGGCTCCATCAGCATCGCGGCGCGCCTCATCCCTCAGCGGATTCCGACGATGGA
GGAACTGAACCTGCCGTCCGTGTTCAAAGCCCTGATGAACCGCCCGTATGGGCTCGTGCTCGTTACCGGGCCGACCGGAA
GCGGCAAGAGCACGACGCTCGCGGCGGTGCTCGATGCCATCAACCAGACGCAGCGCCGGCACATCATTACGTTGGAAGAC
CCGATTGAATACCTGCACACGCACAAGCTGTCGATCATCGACCAGCGCGAGATCGGCCAGGACACCTCGGGCTTCGCGCC
CGCGCTGCGCGCGGCGCTCCGCCAGGATCCAGACATCCTGCTCGTGGGCGAGATGCGCGATCTCGAGACCATCGCGACGG
CCATCACGGCGTCGGAAACCGGTCACCTGGTGTTCGCCACGCTGCACACGTCGGACGCGGTGCAGACGGTGGAGCGCATC
ATCGACGTGTTTCCGCCGGAGCAGCAGGGCCAGATTCGCATCCAGCTCGCAGGCGTGCTCGCGGGCGTCGTCTCCCAGCG
GCTGCACCGCACGCGCGACGGGCGGGGGCGCGTGGCGTCGTTCGAGGTGCTCGTGAACACCCCCGCCGTGGCCAACCTCA
TTCGAAGCGGCAAGACGCACCAGATCGGCGCCATGATGCAGACGGGCCGCCAGTTCGGCATGATCACGATGGAGATGAGC
ATTCGGGAATTGGTGCAGCAGGGGAAGATCTCGCCTGCGGTACTCCGCCAGTGGCAGCCTGCGCAGTTGTCGCAGCAGTC
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

49.704

97.406

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.376

94.236

0.484
  pilT Pseudomonas aeruginosa PAK

49.85

95.965

0.478
  pilT Neisseria gonorrhoeae MS11

48.665

97.118

0.473
  pilT Acinetobacter nosocomialis M2

48.512

96.83

0.47
  pilT Acinetobacter baumannii D1279779

48.512

96.83

0.47
  pilT Acinetobacter baumannii strain A118

48.512

96.83

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.338

95.389

0.461
  pilT Legionella pneumophila strain ERS1305867

47.748

95.965

0.458
  pilT Legionella pneumophila strain Lp02

47.748

95.965

0.458
  pilT Acinetobacter baylyi ADP1

47.734

95.389

0.455
  pilT Vibrio cholerae strain A1552

46.061

95.101

0.438
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.061

95.101

0.438
  pilU Vibrio cholerae strain A1552

44.479

91.354

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.389

0.392
  pilU Acinetobacter baylyi ADP1

38.024

96.254

0.366

Multiple sequence alignment